CATSPER3 (NM_178019) Human Tagged ORF Clone

CAT#: RG210713

CATSPER3 (tGFP-tagged) - Human cation channel, sperm associated 3 (CATSPER3)

Product Images

Circular map for RG210713

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CATSPER3
• Synonyms: CACRC
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG210713 representing NM_178019
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTCAACACCGTCACCAGCGCCACTCGAGAGTCATTTCTAGTTCACCAGTTGACACTACATCGGTGG
  GATTTTGCCCAACATTCAAGAAATTTAAGAGGAACGATGATGAATGTCGGGCATTTGTGAAGAGAGTCAT
  AATGAGCCGTTTCTTTAAGATAATTATGATTAGCACTGTCACATCGAATGCGTTTTTTATGGCCTTGTGG
  ACCAGTTATGACATAAGGTACCGCTTGTTCAGACTTCTTGAGTTCTCGGAGATCTTCTTTGTGTCCATCT
  GCACATCTGAGTTGTCCATGAAGGTCTATGTGGACCCCATCAACTACTGGAAGAACGGCTACAACCTGCT
  GGATGTGATCATTATCATCGTTATGTTTTTACCCTATGCCCTCCGCCAGCTCATGGGCAAACAGTTCACT
  TACCTGTATATCGCTGATGGCATGCAGTCCCTGCGCATCCTCAAGCTTATCGGCTATAGCCAGGGCATCC
  GGACGCTGATCACCGCCGTGGGGCAGACAGTCTACACCGTGGCCTCTGTGCTCCTCCTGCTCTTCCTCCT
  CATGTACATCTTCGCTATCTTGGGCTTCTGCCTGTTTGGATCTCCAGACAATGGTGACCATGATAACTGG
  GGGAACCTGGCTGCGGCTTTTTTCACCCTCTTCAGCTTGGCCACGGTTGATGGCTGGACAGACCTGCAGA
  AGCAGTTGGACAATCGGGAATTTGCTTTGAGCCGGGCATTCACCATCATCTTCATCTTGCTCGCCTCTTT
  CATCTTCCTCAACATGTTCGTGGGTGTGATGATCATGCACACAGAGGACTCCATCAGAAAGTTTGAGCGA
  GAGCTGATGTTGGAGCAGCAGGAGATGCTCATGGGAGAGAAGCAGGTGATTCTGCAGCGGCAGCAGGAGG
  AGATCAGCAGGCTGATGCACATACAGAAAAATGCTGACTGCACAAGTTTCAGTGAGCTGGTGGAGAACTT
  TAAGAAGACCTTGAGCCACACTGACCCAATGGTCTTGGATGATTTTGGCACTAGCTTACCCTTCATCGAT
  ATCTACTTTTCCACTCTGGACTACCAGGACACAACTGTCCACAAGCTTCAAGAGCTGTACTATGAGATCG
  TGCATGTGCTGAGCCTAATGCTGGAAGACTTGCCCCAGGAGAAGCCCCAGTCCTTGGAAAAGGTGGATGA
  GAAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG210713 representing NM_178019
  Red=Cloning site Green=Tags(s)
  
  MSQHRHQRHSRVISSSPVDTTSVGFCPTFKKFKRNDDECRAFVKRVIMSRFFKIIMISTVTSNAFFMALW
  TSYDIRYRLFRLLEFSEIFFVSICTSELSMKVYVDPINYWKNGYNLLDVIIIIVMFLPYALRQLMGKQFT
  YLYIADGMQSLRILKLIGYSQGIRTLITAVGQTVYTVASVLLLLFLLMYIFAILGFCLFGSPDNGDHDNW
  GNLAAAFFTLFSLATVDGWTDLQKQLDNREFALSRAFTIIFILLASFIFLNMFVGVMIMHTEDSIRKFER
  ELMLEQQEMLMGEKQVILQRQQEEISRLMHIQKNADCTSFSELVENFKKTLSHTDPMVLDDFGTSLPFID
  IYFSTLDYQDTTVHKLQELYYEIVHVLSLMLEDLPQEKPQSLEKVDEK
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_178019
• ORF Size: 1194 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_178019.1, NP_821138.1
• RefSeq Size: 1338 bp
• RefSeq ORF: 1197 bp
• Locus ID: 347732
• UniProt ID: Q86XQ3
• Cytogenetics: 5q31.1
• Protein Families: Druggable Genome, Ion Channels: Other, Transmembrane
• Gene Summary: Voltage-gated calcium channel that plays a central role in calcium-dependent physiological responses essential for successful fertilization, such as sperm hyperactivation, acrosome reaction and chemotaxis towards the oocyte.[UniProtKB/Swiss-Prot Function]