CKAP4 (NM_006825) Human Tagged ORF Clone

CAT#: RG210270

CKAP4 (tGFP-tagged) - Human cytoskeleton-associated protein 4 (CKAP4)

Product Images

Circular map for RG210270

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CKAP4
• Synonyms: CLIMP-63; CLIMP63; ERGIC-63; p63
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG210270 representing NM_006825
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCTCGGCCAAACAAAGGGGCTCCAAGGGCGGCCACGGCGCCGCGAGCCCCTCGGAGAAGGGTGCCC
  ACCCGTCGGGCGGCGCGGATGACGTGGCGAAGAAGCCGCCGCCGGCGCCGCAGCAGCCGCCGCCGCCGCC
  CGCGCCGCACCCGCAGCAGCACCCGCAGCAGCACCCGCAGAACCAGGCGCACGGCAAGGGCGGCCACCGC
  GGCGGCGGCGGCGGCGGCGGCAAGTCCTCCTCCTCCTCCTCCGCCTCCGCCGCCGCTGCCGCCGCCGCCG
  CCTCGTCCTCGGCGTCCTGCTCGCGCAGGCTCGGCAGGGCGCTCAACTTTCTCTTCTACCTCGCCCTGGT
  GGCGGCGGCCGCTTTCTCGGGCTGGTGCGTCCACCACGTCCTGGAGGAGGTCCAGCAGGTCCGGCGCAGC
  CACCAGGACTTCTCCCGGCAGAGGGAGGAGCTGGGCCAGGGCTTGCAGGGCGTCGAGCAGAAGGTGCAGT
  CTTTGCAAGCCACATTTGGAACTTTTGAGTCCATCTTGAGAAGCTCCCAACATAAACAAGACCTCACAGA
  GAAAGCTGTGAAGCAAGGGGAGAGTGAGGTCAGCCGGATCAGCGAAGTGCTGCAGAAACTCCAGAATGAG
  ATTCTCAAAGACCTCTCGGATGGGATCCATGTGGTGAAGGACGCCCGGGAGCGGGACTTCACGTCCCTGG
  AGAACACGGTGGAGGAGCGGCTGACGGAGCTCACCAAATCCATCAACGACAACATCGCCATCTTCACAGA
  AGTCCAGAAGAGGAGCCAGAAGGAGATCAATGACATGAAGGCAAAGGTTGCCTCCCTGGAAGAATCTGAG
  GGGAACAAGCAGGATTTGAAAGCCTTAAAGGAAGCTGTGAAGGAGATACAGACCTCAGCCAAGTCCAGAG
  AGTGGGACATGGAGGCCCTGAGAAGTACCCTTCAGACTATGGAGTCTGACATCTACACCGAGGTCCGCGA
  GCTGGTGAGCCTCAAGCAGGAGCAGCAGGCTTTCAAGGAGGCGGCCGACACGGAGCGGCTCGCCCTGCAG
  GCCCTCACGGAGAAGCTTCTCAGGTCTGAGGAGTCCGTCTCCCGCCTCCCGGAGGAGATCCGGAGACTGG
  AGGAAGAGCTCCGCCAGCTGAAGTCCGATTCCCACGGGCCGAAGGAGGACGGAGGCTTCAGACACTCGGA
  AGCCTTTGAGGCACTCCAGCAAAAGAGTCAGGGACTGGACTCCAGGCTCCAGCACGTGGAGGATGGGGTG
  CTCTCCATGCAGGTGGCTTCTGCGCGCCAGACCGAGAGCCTGGAGTCCCTCCTGTCCAAGAGCCAGGAGC
  ACGAGCAGCGCCTGGCCGCCCTGCAGGGGCGCCTGGAAGGCCTCGGGTCCTCAGAGGCAGACCAGGATGG
  CCTGGCCAGCACGGTGAGGAGCCTGGGCGAGACCCAGCTGGTGCTCTACGGTGACGTGGAGGAGCTGAAG
  AGGAGTGTGGGCGAGCTCCCCAGCACCGTGGAATCACTCCAGAAGGTGCAGGAGCAGGTGCACACGCTGC
  TCAGTCAGGACCAAGCCCAGGCCGCCCGTCTGCCTCCTCAGGACTTCCTGGACAGACTTTCTTCTCTAGA
  CAACCTGAAAGCCTCAGTCAGCCAAGTGGAGGCGGACTTGAAAATGCTCAGGACTGCTGTGGACAGTTTG
  GTTGCATACTCGGTCAAAATAGAAACCAACGAGAACAATCTGGAATCAGCCAAGGGTTTACTAGATGACC
  TGAGGAATGATCTGGATAGGTTGTTTGTGAAAGTGGAGAAGATTCACGAAAAGGTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG210270 representing NM_006825
  Red=Cloning site Green=Tags(s)
  
  MPSAKQRGSKGGHGAASPSEKGAHPSGGADDVAKKPPPAPQQPPPPPAPHPQQHPQQHPQNQAHGKGGHR
  GGGGGGGKSSSSSSASAAAAAAAASSSASCSRRLGRALNFLFYLALVAAAAFSGWCVHHVLEEVQQVRRS
  HQDFSRQREELGQGLQGVEQKVQSLQATFGTFESILRSSQHKQDLTEKAVKQGESEVSRISEVLQKLQNE
  ILKDLSDGIHVVKDARERDFTSLENTVEERLTELTKSINDNIAIFTEVQKRSQKEINDMKAKVASLEESE
  GNKQDLKALKEAVKEIQTSAKSREWDMEALRSTLQTMESDIYTEVRELVSLKQEQQAFKEAADTERLALQ
  ALTEKLLRSEESVSRLPEEIRRLEEELRQLKSDSHGPKEDGGFRHSEAFEALQQKSQGLDSRLQHVEDGV
  LSMQVASARQTESLESLLSKSQEHEQRLAALQGRLEGLGSSEADQDGLASTVRSLGETQLVLYGDVEELK
  RSVGELPSTVESLQKVQEQVHTLLSQDQAQAARLPPQDFLDRLSSLDNLKASVSQVEADLKMLRTAVDSL
  VAYSVKIETNENNLESAKGLLDDLRNDLDRLFVKVEKIHEKV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006825
• ORF Size: 1806 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006825.4
• RefSeq Size: 2913 bp
• RefSeq ORF: 1809 bp
• Locus ID: 10970
• UniProt ID: Q07065
• Cytogenetics: 12q23.3
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: High-affinity epithelial cell surface receptor for APF.[UniProtKB/Swiss-Prot Function]