PSD4 (NM_012455) Human Tagged ORF Clone

CAT#: RG209940

PSD4 (tGFP-tagged) - Human pleckstrin and Sec7 domain containing 4 (PSD4)

Product Images

Circular map for RG209940

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PSD4
• Synonyms: EFA6B; TIC
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG209940 representing NM_012455
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGGGTGACTACAGACTCCCTGACCACCCCCAGCCCATGGAAATTCTCAACCTGTACTTGGGAGACA
  GCCTGGAGCCCCACCCAGGAGAGTGCCCAAGGGAAACGTGCAGCCATGAGGATCCACCGGAGCCTTTCGA
  GGAGCAAACCTGGGCCACTGACCCTCCTGAACCTACCAGACAAAATGTTCCTCCCTGGGGCTCCGGTGTG
  GAGCTCACACACCTGGGGAGCTGGGTCCATCAGGACGGGCTGGAGCCTTGCCAGGAGCAAACCCGGGCCA
  CTGACCCTCCTGAATCTACCAGACAAGATGCTCCTCCCTGGGGCTCCGGTGTGGAGCTCACACACCTGGG
  GAGCCCCTCTGCCCAGAGGGAGCACAGGCAGAACACAGCATCACCAGGGTCACCAGTGAACAGCCATCTA
  CCGGGGAGCCCAAAGCAGAACCGGAGCACGTCCACACAGGTAGTGTTCTGGGCAGGCATCCTGCAGGCCC
  AGATGTGTGTCCTAGACCTGGAGGAGGAGCTGGAGAAGACGGAAGGGCTCAAGGCTGGGCTGAAATGCTG
  TCTCCCCACGCCCCCTGTGGACCTCCCCGGGGACACGGGCCTGCACTCCAGCCCACCTGAGAATGAAGAC
  TCAGGGGAAGACAGCAGCGAGCCTGAGGGAGAGGGCCAGGCATGGCTGAGAGAGGGAACCCCAGACTCTT
  CCCCACAGTGGGGAGCTGAGGAGGAGAGCATGTTCTTCAGCAACCCCCTCTTCCTGGCGAGTCCTTGCTC
  AGAGAACAGTGCTTCTGGAGAGTGCTTTTCCTGGGCGGCTTCAGACTCCCATGCAGGTGTGAGGACTGGA
  CCTGAGAGCCCAGCGACTCTGGAGCCTCCCCTCCCAGAAGACACAGTGCTGTGGGAGCTGGAAAGTGAGC
  CAGATTTGGGGGACGGCGCTGCTATCAGTGGGCATTGTACCCCTCCATTCCCTGTGCCCATCTATAAACC
  ACACTCCATCTGCTGGGCCTCAGTGGCTGCCGCTGAGGGGGCTCCTGCAGCACCTCCTGGTCACGGGGAG
  AGTGAGGGAGATAGGCTTGGTCCTGCTCCATCTGCAGCACCGTGTGTGGACGAAGCATTGACCTGGGAAT
  CAGGATGTGTCGGATCTGATCTTGGCCCTGCTGCACATCCTGTGCAACCTTGGGCCTCTCTCAGCCCTGA
  GGGCTGGCAGAGAGGAGGTCCTTTTTGGCCCCAGGTGACTCTTAACTCCCAGGACAGAGATGAGAGGGAG
  GGTGGGCACCCCCAGGAATCTCTTCCCTGCACCTTGGCCCCCTGCCCCTGGAGGAGCCCAGCTTCTTCTC
  CAGAGCCTAGCAGCCCAGAATCTGAGAGCAGAGGCCCTGGTCCCAGGCCCAGCCCTGCATCGTCCCAGGA
  GGGCAGCCCGCAGCTTCAACACCACAGCTCAGGCATTTTGCCCAAGTGGACACTAGATGCTTCACAGTCT
  TCACTCTTGGAGACGGATGGGGAACAGCCAAGTTCCTTGAAGAAAAAGGAGGCAGGGGAGGCCCCAAAAC
  CAGGCGAGGAAGTAAAGAGTGAAGGAACAGCCAGGCCTGCAGAGACTGGAGACGTCCAGCCTGACATTCA
  CCTGACTTCTGCAGAACATGAGAATCTGAGGACACCGATGAACTCTTCTTGGCTTCCTGGGAGCCCTATG
  CCCCAAGCACAGTCCCCAGAGGAAGGCCAGAGACCACCAGCTGGAGACAAGCTAGCTAATGGCGTCAGGA
  ACAACAAGGTAGCCTGGAACTTGGCCTCACGCCTCTATCGCCTGGAGGGCTTCCGGAAGTCTGAAGTGGC
  TGCCTACCTGCAGAAGAACAATGACTTTAGCAGGGCTGTGGCTGAGGAGTACCTGTCCTTCTTCCAGTTT
  GGAGGCCAGAGTCTGGACCGAGCCCTCCGGAGCTTCCTCCAGGCCTTGGTGCTCAGTGGGGAGACTCAGG
  AACGGGAGCGAATCCTCTACCAGTTCTCCAGACGCTTCCACCATTGCAATCCGGGGATCTTCCCCTCAGT
  AGATTCTGTACACACCTTGACATGTGCAATCATGCTGCTTAACACGGACCTGCATGGACAGAACATTGGG
  AAGAGCATGAGCTGCCAGGAATTCATAACCAACCTGAATGGGCTGAGGGATGGCGGGAACTTCCCCAAGG
  AGCTGCTGAAGGCCCTCTACTGGTCTATCCGCAGCGAGAAGCTCGAGTGGGCCGTGGATGAAGAAGACAC
  AGCCAGACCTGAGAAGGCCCAGCCGTCCCTGCCAGCTGGCAAGATGAGCAAGCCCTTCCTTCAGCTGGCT
  CAGGATCCCACAGTGCCCACCTACAAGCAGGGCATCCTGGCTCGGAAAATGCATCAAGATGCAGACGGCA
  AGAAGACGCCATGGGGCAAGCGTGGCTGGAAGATGTTCCACACCTTACTGCGAGGGATGGTTCTCTACTT
  CCTGAAGCAGGGAGAAGACCACTGTCTGGAGGGGGAGAGCTTGGTGGGGCAGATGGTGGATGAGCCCGTG
  GGGGTGCACCACTCGCTGGCCACCCCCGCCACGCATTACACCAAGAAGCCGCACGTCTTCCAGCTGCGCA
  CGGCTGACTGGCGCCTCTACCTCTTCCAGGCACCCACTGCCAAGGAGATGAGCTCCTGGATCGCGCGCAT
  CAACTTGGCTGCGGCCACGCACTCCGCGCCGCCCTTCCCCGCCGCTGTGGGCTCCCAGCGCAGATTCGTG
  CGGCCCATCCTGCCCGTGGGCCCCGCCCAGAGCTCCCTGGAGGAGCAGCATCGATCCCACGAGAACTGCC
  TGGACGCTGCCGCGGACGACCTGCTGGATCTACAGAGGAACCTGCCGGAGCGGCGGGGCCGTGGCCGCGA
  GCTGGAGGAGCACCGCCTGCGGAAGGAGTACCTGGAGTACGAGAAAACCCGCTACGAGACCTACGTGCAG
  CTGCTGGTGGCCCGCCTGCACTGCCCCTCTGATGCTCTGGACCTGTGGGAGGAGCAGCTGGGGAGGGAAG
  CTGGAGGCACTCGGGAGCCCAAGCTCAGCCTGAAGAAGTCCCACTCGAGCCCGTCCCTGCACCAGGATGA
  GGCTCCCACCACGGCCAAGGTGAAGCGCAACATCTCAGAGCGCAGAACCTACCGGAAGATCATCCCTAAG
  CGGAACCGCAATCAGCTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG209940 representing NM_012455
  Red=Cloning site Green=Tags(s)
  
  MMGDYRLPDHPQPMEILNLYLGDSLEPHPGECPRETCSHEDPPEPFEEQTWATDPPEPTRQNVPPWGSGV
  ELTHLGSWVHQDGLEPCQEQTRATDPPESTRQDAPPWGSGVELTHLGSPSAQREHRQNTASPGSPVNSHL
  PGSPKQNRSTSTQVVFWAGILQAQMCVLDLEEELEKTEGLKAGLKCCLPTPPVDLPGDTGLHSSPPENED
  SGEDSSEPEGEGQAWLREGTPDSSPQWGAEEESMFFSNPLFLASPCSENSASGECFSWAASDSHAGVRTG
  PESPATLEPPLPEDTVLWELESEPDLGDGAAISGHCTPPFPVPIYKPHSICWASVAAAEGAPAAPPGHGE
  SEGDRLGPAPSAAPCVDEALTWESGCVGSDLGPAAHPVQPWASLSPEGWQRGGPFWPQVTLNSQDRDERE
  GGHPQESLPCTLAPCPWRSPASSPEPSSPESESRGPGPRPSPASSQEGSPQLQHHSSGILPKWTLDASQS
  SLLETDGEQPSSLKKKEAGEAPKPGEEVKSEGTARPAETGDVQPDIHLTSAEHENLRTPMNSSWLPGSPM
  PQAQSPEEGQRPPAGDKLANGVRNNKVAWNLASRLYRLEGFRKSEVAAYLQKNNDFSRAVAEEYLSFFQF
  GGQSLDRALRSFLQALVLSGETQERERILYQFSRRFHHCNPGIFPSVDSVHTLTCAIMLLNTDLHGQNIG
  KSMSCQEFITNLNGLRDGGNFPKELLKALYWSIRSEKLEWAVDEEDTARPEKAQPSLPAGKMSKPFLQLA
  QDPTVPTYKQGILARKMHQDADGKKTPWGKRGWKMFHTLLRGMVLYFLKQGEDHCLEGESLVGQMVDEPV
  GVHHSLATPATHYTKKPHVFQLRTADWRLYLFQAPTAKEMSSWIARINLAAATHSAPPFPAAVGSQRRFV
  RPILPVGPAQSSLEEQHRSHENCLDAAADDLLDLQRNLPERRGRGRELEEHRLRKEYLEYEKTRYETYVQ
  LLVARLHCPSDALDLWEEQLGREAGGTREPKLSLKKSHSSPSLHQDEAPTTAKVKRNISERRTYRKIIPK
  RNRNQL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012455
• ORF Size: 3168 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012455.2, NP_036587.2
• RefSeq Size: 5039 bp
• RefSeq ORF: 3171 bp
• Locus ID: 23550
• UniProt ID: Q8NDX1
• Cytogenetics: 2q14.1
• Protein Pathways: Endocytosis
• Gene Summary: Guanine nucleotide exchange factor for ARF6 and ARL14/ARF7. Through ARL14 activation, controls the movement of MHC class II-containing vesicles along the actin cytoskeleton in dendritic cells. Involved in membrane recycling. Interacts with several phosphatidylinositol phosphate species, including phosphatidylinositol 3,4-bisphosphate, phosphatidylinositol 3,5-bisphosphate and phosphatidylinositol 4,5-bisphosphate.[UniProtKB/Swiss-Prot Function]