AKAP14 (NM_178813) Human Tagged ORF Clone

CAT#: RG209895

AKAP14 (tGFP-tagged) - Human A kinase (PRKA) anchor protein 14 (AKAP14), transcript variant 1

Product Images

Circular map for RG209895

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: AKAP14
• Synonyms: AKAP28; PRKA14
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG209895 representing NM_178813
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTGAGACTCAAAATTCAACAAGCCAGAAAGCAATGGATGAGGATAACAAAGCCGCAAGCCAAACAA
  TGCCGAATACACAAGACAAGAACTACGAGGATGAATTGACTCAAGTAGCTCTAGCTCTGGTTGAGGATGT
  CATCAATTATGCTGTTAAGATTGTGGAAGAGGAGCGAAACCCTTTGAAAAACATCAAGTGGATGACTCAC
  GGTGAATTCACTGTGGAAAAGGGTCTTAAACAAATTGACGAATATTTTTCGAAGTGTGTTTCTAAAAAAT
  GCTGGGCACATGGCGTAGAGTTTGTAGAGAGGAAAGACTTAATTCACAGCTTCCTCTACATCTACTATGT
  ACACTGGAGTATCTCAACTGCTGACCTACCCGTAGCACGAATCTCTGCTGGTACCTACTTCACCATGAAG
  GTCTCCAAAACCAAACCACCGGATGCACCCATTGTTGTTTCTTATGTAGGTGACCACCAAGCATTAGTTC
  ACAGACCAGGAATGGTTCGCTTTCGAGAAAACTGGCAGAAGAATCTTACTGATGCCAAATATAGTTTCAT
  GGAGTCATTCCCCTTCTTATTCAATCGTGTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG209895 representing NM_178813
  Red=Cloning site Green=Tags(s)
  
  MSETQNSTSQKAMDEDNKAASQTMPNTQDKNYEDELTQVALALVEDVINYAVKIVEEERNPLKNIKWMTH
  GEFTVEKGLKQIDEYFSKCVSKKCWAHGVEFVERKDLIHSFLYIYYVHWSISTADLPVARISAGTYFTMK
  VSKTKPPDAPIVVSYVGDHQALVHRPGMVRFRENWQKNLTDAKYSFMESFPFLFNRV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_178813
• ORF Size: 591 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_178813.6
• RefSeq Size: 855 bp
• RefSeq ORF: 594 bp
• Locus ID: 158798
• UniProt ID: Q86UN6
• Cytogenetics: Xq24
• Protein Families: Druggable Genome
• Gene Summary: The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The protein anchors PKA in ciliary axonemes and, in this way, may play a role in regulating ciliary beat frequency. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]