Cullin 7 (CUL7) (NM_014780) Human Tagged ORF Clone

CAT#: RG207531

CUL7 (tGFP-tagged) - Human cullin 7 (CUL7), transcript variant 2

Product Images

Circular map for RG207531

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Cullin 7
• Synonyms: 3M1; CUL-7; dJ20C7.5; KIAA0076
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG207531 representing NM_014780
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGGGAGAACTCCGCTACAGGGAATTCAGGGTGCCCCTGGGGCCCGGCTTACATGCCTATCCTGATG
  AGCTGATCCGCCAGCGCGTGGGCCATGATGGGCATCCTGAGTACCAGATCCGTTGGCTCATCCTGCGGCG
  TGGCGATGAGGGGGACGGGGGCTCTGGCCAAGTGGACTGCAAGGCTGAGCACATCCTGCTGTGGATGTCC
  AAGGATGAGATCTATGCCAACTGCCACAAGATGCTGGGCGAGGATGGCCAGGTCATCGGGCCCTCCCAGG
  AGTCTGCAGGGGAGGTTGGGGCCCTGGACAAATCTGTGCTGGAGGAGATGGAAACCGACGTGAAGTCCCT
  CATTCAGAGAGCCCTTCGGCAGCTGGAGGAGTGTGTGGGCACTATCCCTCCTGCTCCTCTACTTCACACT
  GTCCACGTGCTCAGCGCCTATGCCAGCATTGAGCCCCTCACTGGAGTATTCAAGGACCCAAGGGTCCTGG
  ACTTGCTCATGCACATGTTGAGTAGTCCTGATTATCAGATTCGCTGGAGTGCAGGCCGGATGATACAAGC
  CCTGTCCTCCCATGACGCTGGGACCCGGACTCAGATCCTTCTGTCACTGAGCCAACAAGAAGCCATTGAG
  AAACACCTGGATTTTGACAGCCGCTGTGCTCTGCTAGCACTGTTTGCACAGGCCACGCTCTCTGAACACC
  CCATGTCTTTCGAGGGCATTCAGCTACCACAGGTCCCAGGAAGGGTGCTCTTCTCCCTGGTGAAGCGGTA
  TTTGCATGTCACCTCGCTCCTGGATCAGCTGAACGACAGTGCTGCGGAGCCAGGAGCCCAGAACACCTCT
  GCTCCTGAGGAGTTGAGTGGGGAGAGGGGTCAACTGGAGCTGGAGTTCAGTATGGCCATGGGCACCCTGA
  TCTCGGAGCTGGTGCAAGCCATGCGCTGGGACCAGGCCTCAGACAGACCAAGGAGCTCAGCACGGTCCCC
  CGGTTCCATCTTCCAGCCTCAGCTGGCAGATGTGAGCCCAGGGCTCCCCGCTGCCCAGGCTCAGCCCTCC
  TTCAGGAGGTCAAGACGTTTTCGCCCTCGTTCTGAGTTCGCAAGTGGCAATACCTATGCTTTGTATGTGC
  GGGACACACTGCAGCCGGGGATGCGAGTGCGGATGCTGGATGATTATGAGGAGATCAGTGCCGGGGATGA
  GGGCGAGTTTCGGCAGAGCAACAACGGTGTGCCTCCTGTGCAGGTATTTTGGGAGTCAACAGGCCGCACC
  TATTGGGTGCACTGGCACATGCTGGAGATCTTGGGCTTTGAGGAAGACATTGAGGACATGGTTGAGGCTG
  ATGAGTACCAAGGGGCAGTGGCCAGTAGAGTCCTGGGTAGAGCCCTGCCTGCCTGGCGCTGGAGGCCCAT
  GACAGAACTCTATGCTGTGCCTTATGTGCTGCCTGAGGATGAGGACACTGAGGAGTGTGAACACCTGACC
  CTGGCTGAGTGGTGGGAACTCCTCTTCTTCATCAAGAAGCTGGATGGACCTGACCATCAGGAGGTTCTCC
  AGATCCTCCAGGAGAACCTAGATGGGGAGATTCTGGATGATGAGATCCTAGCTGAACTGGCCGTGCCCAT
  AGAATTGGCCCAGGACTTGCTGCTGACTCTGCCACAGCGACTCAATGACAGTGCCCTCAGGGACCTGATC
  AACTGCCATGTCTACAAGAAGTATGGGCCTGAAGCCCTAGCAGGGAACCAAGCCTACCCATCCCTTCTAG
  AAGCCCAAGAAGATGTCCTCCTGCTAGACGCGCAGGCCCAGGCTAAGGACTCAGAAGATGCAGCCAAAGT
  GGAAGCAAAAGAACCCCCATCTCAGAGTCCCAACACTCCCCTGCAGCGTCTGGTGGAGGGTTATGGTCCA
  GCTGGGAAAATCCTCCTGGATCTAGAGCAAGCCCTCAGCTCAGAGGGGACCCAGGAGAACAAGGTCAAGC
  CACTCCTGCTGCAGCTGCAGCGGCAGCCGCAGCCCTTCCTGGCACTGATGCAGAGCCTGGACACTCCGGA
  GACTAACAGGACCCTGCACCTGACTGTGCTGAGAATCCTGAAGCAGCTGGTGGACTTCCCCGAGGCACTG
  CTGCTCCCCTGGCACGAGGCCGTGGATGCCTGCATGGCCTGCCTGCGGTCCCCAAACACTGATCGAGAGG
  TGCTCCAGGAACTGATTTTCTTCCTGCACCGCCTGACCTCAGTGAGCAGGGACTATGCCGTGGTGCTGAA
  TCAGCTGGGAGCAAGAGACGCTATCTCCAAGGCCCTGGAAAAGCACCTGGGAAAGCTGGAGCTGGCTCAG
  GAGCTGCGGGACATGGTGTTCAAGTGTGAGAAGCATGCCCACCTCTACCGCAAACTCATCACCAACATCC
  TGGGAGGCTGCATCCAGATGGTGCTGGGCCAGATCGAAGACCACAGACGAACCCACCAACCCATCAACAT
  CCCTTTCTTTGATGTGTTCCTCAGATACCTGTGCCAGGGCTCCAGTGTGGAAGTGAAGGAGGACAAGTGC
  TGGGAGAAGGTGGAGGTGTCCTCCAACCCGCACCGGGCCAGCAAGCTGACGGACCACAACCCCAAGACCT
  ATTGGGAGTCCAACGGCAGCGCCGGCTCCCACTACATCACCCTGCACATGCGCCGGGGCATCCTCATCAG
  GCAACTGACTCTGCTTGTGGCTAGTGAGGACTCGAGTTACATGCCGGCCCGAGTGGTGGTGTGCGGGGGT
  GATAGCACTAGCTCTCTTCACACGGAACTCAACTCGGTGAATGTGATGCCCTCTGCCAGCCGGGTGATCC
  TCCTGGAGAACCTGACCCGCTTCTGGCCCATCATCCAGATCCGCATAAAGCGCTGCCAGCAGGGTGGCAT
  TGATACGCGCATTCGGGGGTTAGAGATCCTAGGCCCCAAGCCCACGTTCTGGCCAGTGTTCCGGGAGCAG
  CTCTGTCGTCACACACGCCTCTTCTACATGGTTCGGGCACAGGCCTGGAGCCAGGACATGGCAGAGGACC
  GCAGGAGCCTCCTGCACCTGAGTTCTAGACTCAACGGTGCTCTGCGCCAGGAGCAGAATTTTGCTGACCG
  CTTCCTCCCTGATGACGAGGCTGCCCAAGCTCTGGGCAAGACCTGCTGGGAGGCCCTGGTCAGCCCCGTG
  GTGCAGAACATCACCTCCCCTGATGAGGATGGCATTAGCCCCCTGGGTTGGCTGCTGGACCAGTACCTGG
  AGTGTCAGGAAGCTGTCTTCAACCCCCAGAGCCGCGGCCCAGCTTTCTTCTCGCGGGTGCGCCGTCTCAC
  TCACCTGCTGGTGCATGTCGAGCCCTGTGAGGCACCCCCTCCTGTGGTGGCCACTCCTCGGCCCAAAGGC
  AGAAACAGAAGCCACGACTGGAGCTCCTTGGCTACCCGGGGCCTTCCAAGCAGCATCATGAGAAACCTGA
  CGCGCTGTTGGCGGGCCGTGGTGGAGAAGCAGGTGAACAATTTTCTGACCTCATCCTGGCGGGATGATGA
  CTTTGTGCCACGCTACTGTGAGCACTTTAATATTCTGCAGAACTCAAGCTCTGAACTGTTTGGGCCTCGG
  GCAGCCTTCTTGCTGGCGCTGCAAAATGGCTGTGCGGGAGCCTTGCTGAAGCTCCCTTTTCTCAAAGCTG
  CCCACGTGAGTGAGCAGTTCGCCCGGCACATTGACCAGCAGATCCAGGGCAGCCGGATCGGTGGAGCCCA
  GGAAATGGAGAGGCTGGCACAGCTGCAGCAATGCCTGCAAGCTGTCCTGATTTTCTCCGGCTTGGAGATA
  GCCACCACTTTTGAGCATTATTACCAGCACTACATGGCGGACCGTCTCCTGGGCGTGGTCTCGAGCTGGC
  TGGAGGGGGCCGTGCTGGAGCAGATCGGTCCCTGCTTCCCCAACCGCCTCCCCCAGCAGATGTTGCAGAG
  CCTGAGCACCTCTAAGGAGCTGCAGCGCCAGTTCCACGTCTACCAGCTCCAGCAGCTGGATCAGGAACTC
  CTGAAGCTGGAGGATACAGAGAAGAAAATACAGGTGGGCCTTGGGGCCAGTGGCAAGGAGCACAAGAGCG
  AGAAGGAAGAGGAAGCTGGGGCAGCAGCAGTGGTGGATGTGGCGGAGGGAGAGGAGGAAGAGGAGGAGAA
  TGAGGACCTCTACTATGAAGGGGCAATGCCAGAAGTGTCTGTGCTTGTCCTGTCCCGACACTCCTGGCCT
  GTTGCCTCAATCTGCCACACACTGAACCCCAGAACCTGCCTGCCCTCCTACCTGAGGGGCACTTTGAACA
  GATACTCCAACTTCTACAACAAGAGTCAGAGCCACCCTGCCCTTGAGCGAGGCTCACAGAGGCGACTGCA
  GTGGACGTGGCTGGGCTGGGCTGAGCTGCAGTTTGGGAACCAGACCCTGCATGTGTCCACCGTGCAGATG
  TGGCTACTGCTGTATCTCAACGACCTGAAGGCGGTCTCTGTGGAGAGTCTGCTGGCGTTCTCAGGGCTCT
  CCGCAGACATGCTCAATCAGGCGATTGGGCCCCTCACCTCTTCAAGAGGCCCCCTGGACCTTCACGAGCA
  AAAGGATATACCAGGAGGGGTCCTCAAGATTCGAGATGGCAGCAAGGAACCCAGGTCGAGATGGGACATT
  GTGCGGCTCATCCCACCTCAGACGTACCTGCAAGCTGAGGGTGAAGACGGCCAGAACTTGGAGAAGAGAC
  GGAATCTTCTGAACTGCCTCATCGTCCGAATCCTCAAGGCCCATGGAGATGAGGGGCTGCACATTGACCA
  GCTTGTCTGTCTGGTGCTGGAGGCTTGGCAGAAGGGCCCGTGTCCTCCCAGGGGTTTGGTCAGCAGCCTT
  GGTAAGGGGTCTGCATGCAGCAGCACTGACGTCCTCTCCTGCATCCTACACCTCCTGGGCAAGGGCACGC
  TGAGACGCCATGACGACCGGCCCCAGGTGCTGTCCTATGCAGTCCCTGTGACTGTCATGGAGCCTCACAC
  TGAGTCCCTGAACCCAGGCTCCTCAGGCCCCAACCCACCCCTCACCTTCCATACCCTACAGATTCGCTCC
  CGGGGTGTGCCCTATGCCTCCTGCACTGCCACCCAGAGCTTCTCTACCTTCCGG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG207531 representing NM_014780
  Red=Cloning site Green=Tags(s)
  
  MVGELRYREFRVPLGPGLHAYPDELIRQRVGHDGHPEYQIRWLILRRGDEGDGGSGQVDCKAEHILLWMS
  KDEIYANCHKMLGEDGQVIGPSQESAGEVGALDKSVLEEMETDVKSLIQRALRQLEECVGTIPPAPLLHT
  VHVLSAYASIEPLTGVFKDPRVLDLLMHMLSSPDYQIRWSAGRMIQALSSHDAGTRTQILLSLSQQEAIE
  KHLDFDSRCALLALFAQATLSEHPMSFEGIQLPQVPGRVLFSLVKRYLHVTSLLDQLNDSAAEPGAQNTS
  APEELSGERGQLELEFSMAMGTLISELVQAMRWDQASDRPRSSARSPGSIFQPQLADVSPGLPAAQAQPS
  FRRSRRFRPRSEFASGNTYALYVRDTLQPGMRVRMLDDYEEISAGDEGEFRQSNNGVPPVQVFWESTGRT
  YWVHWHMLEILGFEEDIEDMVEADEYQGAVASRVLGRALPAWRWRPMTELYAVPYVLPEDEDTEECEHLT
  LAEWWELLFFIKKLDGPDHQEVLQILQENLDGEILDDEILAELAVPIELAQDLLLTLPQRLNDSALRDLI
  NCHVYKKYGPEALAGNQAYPSLLEAQEDVLLLDAQAQAKDSEDAAKVEAKEPPSQSPNTPLQRLVEGYGP
  AGKILLDLEQALSSEGTQENKVKPLLLQLQRQPQPFLALMQSLDTPETNRTLHLTVLRILKQLVDFPEAL
  LLPWHEAVDACMACLRSPNTDREVLQELIFFLHRLTSVSRDYAVVLNQLGARDAISKALEKHLGKLELAQ
  ELRDMVFKCEKHAHLYRKLITNILGGCIQMVLGQIEDHRRTHQPINIPFFDVFLRYLCQGSSVEVKEDKC
  WEKVEVSSNPHRASKLTDHNPKTYWESNGSAGSHYITLHMRRGILIRQLTLLVASEDSSYMPARVVVCGG
  DSTSSLHTELNSVNVMPSASRVILLENLTRFWPIIQIRIKRCQQGGIDTRIRGLEILGPKPTFWPVFREQ
  LCRHTRLFYMVRAQAWSQDMAEDRRSLLHLSSRLNGALRQEQNFADRFLPDDEAAQALGKTCWEALVSPV
  VQNITSPDEDGISPLGWLLDQYLECQEAVFNPQSRGPAFFSRVRRLTHLLVHVEPCEAPPPVVATPRPKG
  RNRSHDWSSLATRGLPSSIMRNLTRCWRAVVEKQVNNFLTSSWRDDDFVPRYCEHFNILQNSSSELFGPR
  AAFLLALQNGCAGALLKLPFLKAAHVSEQFARHIDQQIQGSRIGGAQEMERLAQLQQCLQAVLIFSGLEI
  ATTFEHYYQHYMADRLLGVVSSWLEGAVLEQIGPCFPNRLPQQMLQSLSTSKELQRQFHVYQLQQLDQEL
  LKLEDTEKKIQVGLGASGKEHKSEKEEEAGAAAVVDVAEGEEEEEENEDLYYEGAMPEVSVLVLSRHSWP
  VASICHTLNPRTCLPSYLRGTLNRYSNFYNKSQSHPALERGSQRRLQWTWLGWAELQFGNQTLHVSTVQM
  WLLLYLNDLKAVSVESLLAFSGLSADMLNQAIGPLTSSRGPLDLHEQKDIPGGVLKIRDGSKEPRSRWDI
  VRLIPPQTYLQAEGEDGQNLEKRRNLLNCLIVRILKAHGDEGLHIDQLVCLVLEAWQKGPCPPRGLVSSL
  GKGSACSSTDVLSCILHLLGKGTLRRHDDRPQVLSYAVPVTVMEPHTESLNPGSSGPNPPLTFHTLQIRS
  RGVPYASCTATQSFSTFR
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014780
• ORF Size: 5094 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014780.5
• RefSeq Size: 5520 bp
• RefSeq ORF: 5097 bp
• Locus ID: 9820
• UniProt ID: Q14999
• Cytogenetics: 6p21.1
• Protein Pathways: Ubiquitin mediated proteolysis
• Gene Summary: The protein encoded by this gene is a component of an E3 ubiquitin-protein ligase complex. The encoded protein interacts with TP53, CUL9, and FBXW8 proteins. Defects in this gene are a cause of 3M syndrome type 1 (3M1). Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2009]