ZFYVE27 (NM_144588) Human Tagged ORF Clone
CAT#: RG206193
- TrueORF®
ZFYVE27 (tGFP-tagged) - Human zinc finger, FYVE domain containing 27 (ZFYVE27), transcript variant 2
ORF Plasmid: DDK
Lentiviral Particles: DDK DDK w/ Puro mGFP mGFP w/ Puro
AAV Particle: DDK
"NM_144588" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | ZFYVE27 |
Synonyms | PROTRUDIN; SPG33 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG206193 representing NM_144588
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGACATCAGAACGTGAGGGGAGTGGGCCGGAGCTGAGCCCCAGCGTGATGCCCGAGGCTCCCCTGG AGTCTCCACCTTTTCCTACCAAGTCCCCAGCGTTTGACCTTTTCAACTTGGTTCTCTCCTACAAGAGGCT GGAGATCAACCTGGAACCCTTGAAGGATGCAGGTGATGGTGTTCGATACTTGCTCAGGTGGCAGATGCCT TTGTGTTCCTTGCTGACCTGCCTGGGCCTCAACGTCTTGTTCCTCACTTTGAATGAGGGTGCATGGTACT CAGTAGGTGCCCTGATGATTTCAGTGCCCGCCCTGCTGGGCTACCTTCAGGAGGTTTGCCGGGCACGGCT GCCTGATTCCGAGCTGATGCGGAGGAAGTATCATAGCGTGAGGCAGGAGGACCTGCAGAGAGTTCGCCTG TCTCGTCCCGAGGCCGTGGCTGAGGTGAAGAGCTTCTTGATCCAGCTGGAGGCCTTCCTGAGCCGCCTGT GCTGCACATGTGAAGCCGCCTACCGCGTGCTGCACTGGGAGAACCCCGTCGTGTCCTCACAGTTCTATGG GGCTCTTCTGGGCACAGTCTGCATGCTGTATTTGCTGCCACTCTGCTGGGTTCTCACCCTTTTAAACAGC ACGCTCTTTCTGGGGAATGTGGAGTTCTTCCGAGTTGTGTCTGAGTACAGGGCATCTCTGCAGCAGAGGA TGAACCCAAAGCAGGAAGAGCATGCCTTTGAGAGTCCTCCACCACCAGATGTTGGGGGGAAGGATGGTCT GATGGACAGCACGCCTGCCCTCACACCCACGGAGGACCTCACACCGGGCAGCGTGGAGGAGGCTGAGGAG GCTGAGCCAGATGAAGAGTTTAAAGATGCGATTGAGGAGACCCACTTGGTGGTGCTGGAGGATGATGAGG GCGCCCCGTGCCCAGCAGAGGATGAGCTGGCCCTGCAGGACAACGGGTTCCTGAGCAAGAATGAGGTGCT GCGCAGCAAGGTGTCTCGGCTCACGGAGCGGCTCCGCAAGCGCTACCCCACCAACAACTTCGGGAACTGC ACGGGCTGCTCGGCCACCTTCTCAGTGCTGAAGAAGAGGCGGAGCTGCAGTAATTGTGGAAACAGCTTCT GCTCTCGATGCTGCTCCTTCAAGGTGCCCAAGTCATCCATGGGGGCCACAGCCCCTGAAGCCCAGAGGGA GACTGTGTTTGTGTGTGCCTCGTGTAACCAGACCTTGAGCAAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG206193 representing NM_144588
Red=Cloning site Green=Tags(s) MQTSEREGSGPELSPSVMPEAPLESPPFPTKSPAFDLFNLVLSYKRLEINLEPLKDAGDGVRYLLRWQMP LCSLLTCLGLNVLFLTLNEGAWYSVGALMISVPALLGYLQEVCRARLPDSELMRRKYHSVRQEDLQRVRL SRPEAVAEVKSFLIQLEAFLSRLCCTCEAAYRVLHWENPVVSSQFYGALLGTVCMLYLLPLCWVLTLLNS TLFLGNVEFFRVVSEYRASLQQRMNPKQEEHAFESPPPPDVGGKDGLMDSTPALTPTEDLTPGSVEEAEE AEPDEEFKDAIEETHLVVLEDDEGAPCPAEDELALQDNGFLSKNEVLRSKVSRLTERLRKRYPTNNFGNC TGCSATFSVLKKRRSCSNCGNSFCSRCCSFKVPKSSMGATAPEAQRETVFVCASCNQTLSK TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_144588 |
ORF Size | 1233 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_144588.4, NP_653189.3 |
RefSeq Size | 3030 bp |
RefSeq ORF | 1236 bp |
Locus ID | 118813 |
UniProt ID | Q5T4F4 |
Cytogenetics | 10q24.2 |
Domains | FYVE |
Protein Families | Transmembrane |
Gene Summary | This gene encodes a protein with several transmembrane domains, a Rab11-binding domain and a lipid-binding FYVE finger domain. The encoded protein appears to promote neurite formation. A mutation in this gene has been reported to be associated with hereditary spastic paraplegia, however the pathogenicity of the mutation, which may simply represent a polymorphism, is unclear. [provided by RefSeq, Mar 2010] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC206193 | ZFYVE27 (Myc-DDK-tagged)-Human zinc finger, FYVE domain containing 27 (ZFYVE27), transcript variant 2 |
USD 457.00 |
|
RC206193L1 | Lenti ORF clone of Human zinc finger, FYVE domain containing 27 (ZFYVE27), transcript variant 2, Myc-DDK-tagged |
USD 757.00 |
|
RC206193L2 | Lenti ORF clone of Human zinc finger, FYVE domain containing 27 (ZFYVE27), transcript variant 2, mGFP tagged |
USD 757.00 |
|
RC206193L3 | Lenti ORF clone of Human zinc finger, FYVE domain containing 27 (ZFYVE27), transcript variant 2, Myc-DDK-tagged |
USD 757.00 |
|
RC206193L4 | Lenti ORF clone of Human zinc finger, FYVE domain containing 27 (ZFYVE27), transcript variant 2, mGFP tagged |
USD 757.00 |
|
SC310427 | ZFYVE27 (untagged)-Human zinc finger, FYVE domain containing 27 (ZFYVE27), transcript variant 2 |
USD 503.00 |
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