LARGE2 (NM_152312) Human Tagged ORF Clone

CAT#: RG206132

GYLTL1B (tGFP-tagged) - Human glycosyltransferase-like 1B (GYLTL1B)

Product Images

Circular map for RG206132

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: LARGE2
• Synonyms: GYLTL1B; PP5656
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG206132 representing NM_152312
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCCCCGAGGGCGCCCCCGGGCGCTGGGGGCCGCCGCGCTGTTGCTGCTGCTGCTGCTGCTCGGAT
  TCCTCCTGTTCGGTGGGGACCTGGGGTGTGAGCGCCGCAAGCCTGGCGGGCGAGCGGGGGCCCCGGGATG
  CTTCCCCGGCCCGCTCATGCCACGTGTCCCCCCAGACGGGAGGCTGCGGAGAGCCGCCGCCCTCGACGGA
  GACCCGGGGGCCGGCCCCGGGGACCACAACCGCTCCGACTGCGGCCCGCAGCCGCCGCCGCCGCCCAAGT
  GCGAGCTCTTGCATGTGGCCATCGTGTGTGCGGGGCATAACTCCAGCCGAGACGTCATCACCCTGGTGAA
  GTCCATGCTCTTCTACAGGAAAAATCCACTGCACCTCCACTTGGTGACTGACGCCGTGGCCAGAAACATC
  CTGGAGACGCTCTTCCACACATGGATGGTGCCTGCTGTCCGTGTCAGCTTTTATCATGCCGACCAGCTCA
  AGCCCCAGGTCTCCTGGATCCCCAACAAGCACTACTCCGGCCTCTATGGGCTAATGAAGCTGGTGCTGCC
  CAGTGCCTTGCCTGCTGAGCTGGCCCGCGTCATTGTCCTGGACACGGATGTCACCTTCGCCTCTGACATC
  TCGGAGCTCTGGGCCCTCTTTGCTCACTTTTCTGACACGCAGGCGATCGGTCTTGTGGAGAACCAGAGTG
  ACTGGTACCTGGGCAACCTCTGGAAGAACCACAGGCCCTGGCCTGCCTTGGGCCGGGGATTTAACACAGG
  TGTGATCCTGCTGCGGCTGGACCGGCTCCGGCAGGCTGGCTGGGAGCAGATGTGGAGGCTGACAGCCAGG
  CGGGAGCTCCTTAGCCTGCCTGCCACCTCACTGGCTGACCAGGACATCTTCAACGCTGTGATCAAGGAGC
  ACCCGGGGCTAGTGCAGCGTCTGCCTTGTGTCTGGAATGTGCAGCTGTCAGATCACACACTGGCCGAGCG
  CTGCTACTCTGAGGCGTCTGACCTCAAGGTGATCCACTGGAACTCACCAAAGAAGCTTCGGGTGAAGAAC
  AAGCATGTGGAATTCTTCCGCAATTTCTACCTGACCTTCCTGGAGTACGATGGGAACCTGCTGCGGAGAG
  AGCTCTTTGTGTGCCCCAGCCAGCCCCCACCTGGTGCTGAGCAGTTGCAGCAGGCCCTGGCACAACTGGA
  CGAGGAAGACCCCTGCTTTGAGTTCCGGCAGCAGCAGCTCACTGTGCACCGTGTGCATGTCACTTTCCTG
  CCCCATGAACCGCCACCCCCCCGGCCTCACGATGTCACCCTTGTGGCCCAGCTGTCCATGGACCGGCTGC
  AGATGTTGGAAGCCCTGTGCAGGCACTGGCCTGGCCCCATGAGCCTGGCCTTGTACCTGACAGACGCAGA
  AGCTCAGCAGTTCCTGCATTTCGTCGAGGCCTCACCAGTGCTTGCTGCCCGGCAGGACGTGGCCTACCAT
  GTGGTGTACCGTGAGGGGCCCCTATACCCCGTCAACCAGCTTCGCAACGTGGCCTTGGCCCAGGCCCTCA
  CGCCTTACGTCTTCCTCAGTGACATTGACTTCCTGCCTGCCTATTCTCTCTACGACTACCTCAGGGCCTC
  CATTGAGCAGCTGGGGCTGGGCAGCCGGCGCAAGGCAGCACTGGTGGTGCCGGCATTTGAGACCCTGCGC
  TACCGCTTCAGCTTCCCCCATTCCAAGGTGGAGCTGTTGGCCTTGCTGGATGCGGGCACTCTCTACACCT
  TCAGGTACCACGAGTGGCCCCGAGGCCACGCACCCACAGACTATGCCCGCTGGCGGGAGGCTCAGGCCCC
  GTACCGTGTGCAATGGGCGGCCAACTATGAACCCTACGTGGTGGTGCCACGAGACTGTCCCCGCTATGAT
  CCTCGCTTTGTGGGCTTCGGCTGGAACAAAGTGGCCCACATTGTGGAGCTGGATGCCCAGGAATATGAGC
  TCCTGGTGCTGCCCGAGGCCTTCACCATCCATCTGCCCCACGCTCCAAGCCTGGACATCTCCCGCTTCCG
  CTCCAGCCCCACCTATCGTGACTGCCTCCAGGCCCTCAAGGACGAATTCCACCAGGACTTGTCCCGCCAC
  CATGGGGCTGCTGCCCTCAAATACCTCCCAGCCCTGCAGCAGCCCCAGAGCCCTGCCCGAGGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG206132 representing NM_152312
  Red=Cloning site Green=Tags(s)
  
  MLPRGRPRALGAAALLLLLLLLGFLLFGGDLGCERRKPGGRAGAPGCFPGPLMPRVPPDGRLRRAAALDG
  DPGAGPGDHNRSDCGPQPPPPPKCELLHVAIVCAGHNSSRDVITLVKSMLFYRKNPLHLHLVTDAVARNI
  LETLFHTWMVPAVRVSFYHADQLKPQVSWIPNKHYSGLYGLMKLVLPSALPAELARVIVLDTDVTFASDI
  SELWALFAHFSDTQAIGLVENQSDWYLGNLWKNHRPWPALGRGFNTGVILLRLDRLRQAGWEQMWRLTAR
  RELLSLPATSLADQDIFNAVIKEHPGLVQRLPCVWNVQLSDHTLAERCYSEASDLKVIHWNSPKKLRVKN
  KHVEFFRNFYLTFLEYDGNLLRRELFVCPSQPPPGAEQLQQALAQLDEEDPCFEFRQQQLTVHRVHVTFL
  PHEPPPPRPHDVTLVAQLSMDRLQMLEALCRHWPGPMSLALYLTDAEAQQFLHFVEASPVLAARQDVAYH
  VVYREGPLYPVNQLRNVALAQALTPYVFLSDIDFLPAYSLYDYLRASIEQLGLGSRRKAALVVPAFETLR
  YRFSFPHSKVELLALLDAGTLYTFRYHEWPRGHAPTDYARWREAQAPYRVQWAANYEPYVVVPRDCPRYD
  PRFVGFGWNKVAHIVELDAQEYELLVLPEAFTIHLPHAPSLDISRFRSSPTYRDCLQALKDEFHQDLSRH
  HGAAALKYLPALQQPQSPARG
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_152312
• ORF Size: 2163 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_152312.3, NP_689525.3
• RefSeq Size: 2539 bp
• RefSeq ORF: 2166 bp
• Locus ID: 120071
• UniProt ID: Q8N3Y3
• Cytogenetics: 11p11.2
• Protein Families: Transmembrane
• Gene Summary: Bifunctional glycosyltransferase with both xylosyltransferase and beta-1,3-glucuronyltransferase activities involved in the biosynthesis of the phosphorylated O-mannosyl trisaccharide (N-acetylgalactosamine-beta-3-N-acetylglucosamine-beta-4-(phosphate-6-)mannose), a carbohydrate structure present in alpha-dystroglycan (DAG1). Phosphorylated O-mannosyl trisaccharid is required for binding laminin G-like domain-containing extracellular proteins with high affinity. Elongates the glucuronyl-beta-1,4-xylose-beta disaccharide primer structure by adding repeating units [-3-Xylose-alpha-1,3-GlcA-beta-1-] to produce a heteropolysaccharide. Has a higher activity toward alpha-dystroglycan than LARGE.[UniProtKB/Swiss-Prot Function]