Kir7.1 (KCNJ13) (NM_002242) Human Tagged ORF Clone

CAT#: RG206128

KCNJ13 (tGFP-tagged) - Human potassium inwardly-rectifying channel, subfamily J, member 13 (KCNJ13), transcript variant 1

Product Images

Circular map for RG206128

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Kir7.1
• Synonyms: KIR1.4; KIR7.1; LCA16; SVD
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG206128 representing NM_002242
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACAGCAGTAATTGCAAAGTTATTGCTCCTCTCCTAAGTCAAAGATACCGGAGGATGGTCACCAAGG
  ATGGCCACAGCACACTTCAAATGGATGGCGCTCAAAGAGGTCTTGCATATCTTCGAGATGCTTGGGGAAT
  CCTAATGGACATGCGCTGGCGTTGGATGATGTTGGTCTTTTCTGCTTCTTTTGTTGTCCACTGGCTTGTC
  TTTGCAGTGCTCTGGTATGTTCTGGCTGAGATGAATGGTGATCTGGAACTAGATCATGATGCCCCACCTG
  AAAACCACACTATCTGTGTCAAGTATATCACCAGTTTCACAGCTGCATTCTCCTTCTCCCTGGAGACACA
  ACTCACAATTGGTTATGGTACCATGTTCCCCAGTGGTGACTGTCCAAGTGCAATCGCCTTACTTGCCATA
  CAAATGCTCCTAGGCCTCATGCTAGAGGCTTTTATCACAGGTGCTTTTGTGGCGAAGATTGCCCGGCCAA
  AAAATCGAGCTTTTTCAATTCGCTTTACTGACATAGCAGTAGTAGCTCACATGGATGGCAAACCTAATCT
  TATCTTCCAAGTGGCCAACACCCGACCTAGCCCTCTAACCAGTGTCCGGGTCTCAGCTGTACTCTATCAG
  GAAAGAGAAAATGGCAAACTCTACCAGACCAGTGTGGATTTCCACCTTGATGGCATCAGTTCTGACGAAT
  GTCCATTCTTCATCTTTCCACTAACGTACTATCACTCCATTACACCATCAAGTCCTCTGGCTACTCTGCT
  CCAGCATGAAAATCCTTCTCACTTTGAATTAGTTGTATTCCTTTCAGCAATGCAGGAGGGCACTGGAGAA
  ATATGCCAAAGGAGGACATCCTACCTACAGTCTGAAATCATGTTACATCACTGTTTTGCATCTCTGTTGA
  CCCGAGGTTCCAAATGTGAATATCAAATCAAGATGGAGAATTTTGACAAGACTGTCCCTGAATTTCCAAC
  TCCTCTGGTTTCTAAAAGCCCAAACAGGACTGACCTGGATATCCACATCAATGGACAAAGCATTGACAAT
  TTTCAGATCTCTGAAACAGGACTGACAGAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG206128 representing NM_002242
  Red=Cloning site Green=Tags(s)
  
  MDSSNCKVIAPLLSQRYRRMVTKDGHSTLQMDGAQRGLAYLRDAWGILMDMRWRWMMLVFSASFVVHWLV
  FAVLWYVLAEMNGDLELDHDAPPENHTICVKYITSFTAAFSFSLETQLTIGYGTMFPSGDCPSAIALLAI
  QMLLGLMLEAFITGAFVAKIARPKNRAFSIRFTDIAVVAHMDGKPNLIFQVANTRPSPLTSVRVSAVLYQ
  ERENGKLYQTSVDFHLDGISSDECPFFIFPLTYYHSITPSSPLATLLQHENPSHFELVVFLSAMQEGTGE
  ICQRRTSYLQSEIMLHHCFASLLTRGSKCEYQIKMENFDKTVPEFPTPLVSKSPNRTDLDIHINGQSIDN
  FQISETGLTE
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002242
• ORF Size: 1080 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002242.2, NP_002233.1
• RefSeq Size: 2981 bp
• RefSeq ORF: 1083 bp
• Locus ID: 3769
• UniProt ID: O60928
• Cytogenetics: 2q37.1
• Protein Families: Druggable Genome, Ion Channels: Potassium, Transmembrane
• Gene Summary: This gene encodes a member of the inwardly rectifying potassium channel family of proteins. Members of this family form ion channel pores that allow potassium ions to pass into a cell. The encoded protein belongs to a subfamily of low signal channel conductance proteins that have a low dependence on potassium concentration. Mutations in this gene are associated with snowflake vitreoretinal degeneration. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Feb 2010]