BHMT2 (NM_017614) Human Tagged ORF Clone
CAT#: RG204661
- TrueORF®
BHMT2 (tGFP-tagged) - Human betaine--homocysteine S-methyltransferase 2 (BHMT2), transcript variant 1
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_017614" in other vectors (5)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | BHMT2 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG204661 representing NM_017614
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCACCTGCTGGACGCCCGGGGGCCAAGAAGGGGATTTTGGAGCGCCTGGAGAGTGGGGAGGTTGTGA TTGGAGATGGCAGCTTTCTCATTACTCTGGAGAAGAGAGGCTATGTGAAGGCTGGGCTCTGGACTCCAGA GGCAGTGATAGAACACCCAGACGCAGTTCGTCAACTTCACATGGAATTCTTGAGAGCAGGATCAAATGTC ATGCAGACTTTTACCTTTTCTGCCAGTGAGGACAATATGGAAAGCAAGTGGGAAGATGTAAATGCTGCTG CCTGTGACCTCGCCAGGGAAGTGGCTGGCAAAGGTGATGCTTTGGTAGCAGGGGGGATCTGCCAGACATC AATATACAAATACCAGAAGGATGAAGCTAGAATTAAAAAACTTTTTCGACAACAGCTAGAAGTTTTTGCC TGGAAAAATGTGGACTTCTTGATTGCAGAGTATTTTGAGCACGTTGAAGAAGCTGTGTGGGCTGTGGAAG TCTTAAAAGAATCAGATAGACCCGTGGCAGTTACCATGTGCATAGGCCCAGAGGGAGACATGCATGATAT AACCCCCGGAGAATGTGCTGTGAGGCTGGTGAAGGCAGGGGCTTCCATCGTTGGCGTGAACTGCCGCTTT GGGCCCGACACCAGCTTGAAGACGATGGAGCTCATGAAGGAGGGTCTTGAGTGGGCAGGGCTGAAAGCGC ACCTCATGGTGCAGCCTCTGGGGTTCCACGCGCCTGACTGTGGCAAAGAGGGGTTTGTGGATCTCCCAGA ATATCCCTTTGGACTGGAGTCCAGAGTTGCCACCAGATGGGATATTCAAAAATACGCCAGAGAGGCCTAC AACCTGGGGGTCAGGTACATTGGCGGGTGCTGTGGATTTGAGCCCTACCACATCAGGGCAATTGCAGAGG AGCTGGCCCCAGAAAGGGGCTTTTTGCCACCAGCTTCAGAAAAACACGGCAGCTGGGGAAGTGGTTTGGA CATGCACACCAAACCCTGGATTAGAGCAAGGGCTCGAAGGGAGTATTGGGAGAATCTGCTGCCAGCTTCA GGCAGACCTTTCTGTCCTTCGCTGTCAAAGCCAGACTTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG204661 representing NM_017614
Red=Cloning site Green=Tags(s) MAPAGRPGAKKGILERLESGEVVIGDGSFLITLEKRGYVKAGLWTPEAVIEHPDAVRQLHMEFLRAGSNV MQTFTFSASEDNMESKWEDVNAAACDLAREVAGKGDALVAGGICQTSIYKYQKDEARIKKLFRQQLEVFA WKNVDFLIAEYFEHVEEAVWAVEVLKESDRPVAVTMCIGPEGDMHDITPGECAVRLVKAGASIVGVNCRF GPDTSLKTMELMKEGLEWAGLKAHLMVQPLGFHAPDCGKEGFVDLPEYPFGLESRVATRWDIQKYAREAY NLGVRYIGGCCGFEPYHIRAIAEELAPERGFLPPASEKHGSWGSGLDMHTKPWIRARARREYWENLLPAS GRPFCPSLSKPDF TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_017614 |
ORF Size | 1089 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_017614.5 |
RefSeq Size | 2007 bp |
RefSeq ORF | 1092 bp |
Locus ID | 23743 |
UniProt ID | Q9H2M3 |
Cytogenetics | 5q14.1 |
Domains | S-methyl_trans |
Gene Summary | Homocysteine is a sulfur-containing amino acid that plays a crucial role in methylation reactions. Transfer of the methyl group from betaine to homocysteine creates methionine, which donates the methyl group to methylate DNA, proteins, lipids, and other intracellular metabolites. The protein encoded by this gene is one of two methyl transferases that can catalyze the transfer of the methyl group from betaine to homocysteine. Anomalies in homocysteine metabolism have been implicated in disorders ranging from vascular disease to neural tube birth defects such as spina bifida. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2010] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC204661 | BHMT2 (Myc-DDK-tagged)-Human betaine--homocysteine S-methyltransferase 2 (BHMT2), transcript variant 1 |
USD 457.00 |
|
RC204661L3 | Lenti ORF clone of Human betaine--homocysteine S-methyltransferase 2 (BHMT2), transcript variant 1, Myc-DDK-tagged |
USD 757.00 |
|
RC204661L4 | Lenti ORF clone of Human betaine--homocysteine S-methyltransferase 2 (BHMT2), transcript variant 1, mGFP tagged |
USD 757.00 |
|
SC124281 | BHMT2 (untagged)-Human betaine--homocysteine S-methyltransferase 2 (BHMT2), transcript variant 1 |
USD 457.00 |
|
SC321102 | BHMT2 (untagged)-Human betaine--homocysteine S-methyltransferase 2 (BHMT2), transcript variant 1 |
USD 457.00 |
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