GMEB1 (NM_024482) Human Tagged ORF Clone
CAT#: RG200152
- TrueORF®
GMEB1 (tGFP-tagged) - Human glucocorticoid modulatory element binding protein 1 (GMEB1), transcript variant 2
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_024482" in other vectors (4)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | GMEB1 |
Synonyms | P96PIF; PIF96 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG200152 representing NM_024482
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTAATGCAGAAGTGAGTGTCCCAGTGGGGGATGTGGTTGTGGTACCTACTGAAGGAAATGAAGGGG AGAATCCTGAAGACACTAAAACCCAAGTGATTTTGCAGTTACAGCCTGTGCAACAAGGGATTTATGAAGC TGGGTCGGAGAACAACACGGCAGTTGTAGCAGTAGAAACTCACACGATACACAAAATTGAAGAAGGGATT GATACAGGCACTATAGAAGCAAATGAGGATATGGAAATTGCTTACCCCATAACTTGTGGGGAGAGCAAAG CCATCCTCCTCTGGAAGAAGTTTGTATGTCCAGGAATAAACGTGAAGTGTGTCAAGTTCAATGATCAGTT GATCAGCCCCAAGCACTTTGTTCATCTGGCTGGCAAGTCCACTCTGAAGGACTGGAAGAGAGCTATTCGT CTGGGTGGGATCATGCTCAGGAAAATGATGGACTCCGGACAGATTGATTTTTACCAACATGACAAAGTTT GCTCCAATACCTGCAGAAGCACCAAATTTGATCTTCTGATCAGCAGTGCAAGAGCTCCAGTGCCAGGACA GCAGACAAGTGTGGTGCAGACACCCACTTCGGCTGATGGTAGCATCACGCAGATTGCCATCTCAGAAGAG AGCATGGAAGAGGCAGGGCTGGAATGGAACTCAGCTCTCACCGCTGCTGTCACCATGGCCACGGAGGAGG GTGTAAAGAAAGACTCAGAGGAAATTTCAGAGGACACTTTGATGTTCTGGAAAGGAATAGCTGATGTAGG GCTGATGGAAGAGGTTGTCTGCAATATACAGAAGGAAATAGAGGAGCTACTCAGGGGAGTTCAGCAGCGG CTCATCCAGGCTCCCTTCCAAGTCACAGATGCTGCTGTTCTCAACAATGTAGCACACACATTTGGCCTAA TGGACACAGTCAAGAAGGTTTTAGACAACAGAAGGAACCAAGTAGAGCAGGGAGAAGAACAGTTTCTCTA TACTCTGACAGACTTGGAACGCCAGTTGGAGGAGCAGAAGAAGCAAGGCCAGGATCACAGGCTGAAATCT CAGACAGTTCAAAATGTGGTACTGATGCCTGTGAGCACTCCTAAGCCTCCAAAAAGGCCCCGGCTCCAGC GGCCAGCCTCCACCACTGTCTTGAGCCCTTCTCCTCCTGTCCAGCAGCCTCAGTTCACAGTCATCTCACC CATCACCATCACCCCAGTGGGTCAGTCATTTTCCATGGGCAATATTCCAGTGGCCACCCTCAGCCAGGGC TCCAGTCCTGTGACTGTCCACACACTGCCTTCTGGCCCTCAGCTCTTCCGCTATGCCACAGTGGTCTCCT CTGCCAAGAGCAGCTCACCAGACACAGTGACCATCCACCCTTCATCTAGCTTGGCGCTGCTGAGCTCTAC TGCCATGCAGGATGGGAGTACACTGGGCAACATGACCACCATGGTTAGCCCTGTGGAATTGGTGGCCATG GAGTCCGGCCTAACCTCGGCAATTCAGGCTGTTGAAAGCACCTCAGAGGATGGGCAGACCATCATTGAGA TTGATCCAGCCCCGGACCCAGAAGCTGAAGATACTGAGGGCAAAGCAGTCATCTTGGAGACAGAGCTGAG GACTGAGGAGAAAGTTGTGGCTGAGATGGAAGAACACCAGCATCAAGTTCACAATGTGGAGATTGTGGTC TTAGAGGAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG200152 representing NM_024482
Red=Cloning site Green=Tags(s) MANAEVSVPVGDVVVVPTEGNEGENPEDTKTQVILQLQPVQQGIYEAGSENNTAVVAVETHTIHKIEEGI DTGTIEANEDMEIAYPITCGESKAILLWKKFVCPGINVKCVKFNDQLISPKHFVHLAGKSTLKDWKRAIR LGGIMLRKMMDSGQIDFYQHDKVCSNTCRSTKFDLLISSARAPVPGQQTSVVQTPTSADGSITQIAISEE SMEEAGLEWNSALTAAVTMATEEGVKKDSEEISEDTLMFWKGIADVGLMEEVVCNIQKEIEELLRGVQQR LIQAPFQVTDAAVLNNVAHTFGLMDTVKKVLDNRRNQVEQGEEQFLYTLTDLERQLEEQKKQGQDHRLKS QTVQNVVLMPVSTPKPPKRPRLQRPASTTVLSPSPPVQQPQFTVISPITITPVGQSFSMGNIPVATLSQG SSPVTVHTLPSGPQLFRYATVVSSAKSSSPDTVTIHPSSSLALLSSTAMQDGSTLGNMTTMVSPVELVAM ESGLTSAIQAVESTSEDGQTIIEIDPAPDPEAEDTEGKAVILETELRTEEKVVAEMEEHQHQVHNVEIVV LED TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_024482 |
ORF Size | 1689 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_024482.3 |
RefSeq Size | 1944 bp |
RefSeq ORF | 1692 bp |
Locus ID | 10691 |
UniProt ID | Q9Y692 |
Cytogenetics | 1p35.3 |
Domains | SAND |
Protein Families | Druggable Genome, Transcription Factors |
Gene Summary | This gene encodes a member of KDWK gene family which associates with GMEB2 protein. The GMEB1-GMEB2 complex is essential for parvovirus DNA replication. Studies in rat for a similar gene suggest that this gene's role is to modulate the transactivation of the glucocorticoid receptor when it is bound to glucocorticoid response elements. Three alternative spliced transcript variants encoding different isoforms exist. [provided by RefSeq, Feb 2016] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC200152 | GMEB1 (Myc-DDK-tagged)-Human glucocorticoid modulatory element binding protein 1 (GMEB1), transcript variant 2 |
USD 524.00 |
|
RC200152L3 | Lenti ORF clone of Human glucocorticoid modulatory element binding protein 1 (GMEB1), transcript variant 2, Myc-DDK-tagged |
USD 824.00 |
|
RC200152L4 | Lenti ORF clone of Human glucocorticoid modulatory element binding protein 1 (GMEB1), transcript variant 2, mGFP tagged |
USD 824.00 |
|
SC110190 | GMEB1 (untagged)-Human glucocorticoid modulatory element binding protein 1 (GMEB1), transcript variant 2 |
USD 525.00 |
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