COBLL1 (NM_001278460) Human Tagged ORF Clone

CAT#: RC240024

COBLL1 (myc-DDK-tagged) - Human cordon-bleu WH2 repeat protein-like 1 (COBLL1), transcript variant 3

Product Images

Circular map for RC240024

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: COBLL1
• Synonyms: COBLR1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC240024 representing NM_001278460
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGCCGAGCTGGTCCCCACTTATGTGCGGCCGTGCTGCAGAGGCGGCGGCGAGCTCCCGGACTCCGG
  GCAGGGAAATGGGGCAGGCAGTGACGCGCCGCCTGGGAGCTGGAGCCCGCGCAGCGCCCCGCAGGGCGAT
  GGACGGCCGAACCCCGCGCCCGCAGGACGCCCCAGCCAGGAGAAAACCAAAAGCCAAGGCACCACTTCCT
  CCAGCTGAGACCAAATATACTGATGTCTCTTCAGCTGCTGATTCTGTAGAATCCACTGCTTTCATCATGG
  AACAGAAAGAAAACATGATAGATAAAGACGTTGAACTCTCAGTGGTCCTACCTGGGGATATTATCAAATC
  TACTACTGTTCATGGCAGTAAACCTATGATGGACTTGTTGATATTCCTTTGTGCACAGTATCACTTAAAT
  CCATCAAGTTACACAATCGATCTGTTGTCAGCTGAACAGAACCACATTAAATTTAAGCCAAACACACCAA
  TAGGAATGTTGGAGGTAGAGAAGGTAATTTTAAAGCCAAAAATGTTGGATAAGAAAAAACCTACACCTAT
  AATACCAGAGAAAACTGTGAGAGTAGTGATTAATTTTAAGAAAACACAGAAGACCATAGTGAGAGTGAGT
  CCACATGCATCGCTTCAAGAGCTTGCCCCTATTATATGTAGCAAATGTGAGTTTGATCCGTTGCATACAC
  TATTGTTGAAAGATTATCAATCGCAGGAGCCTCTTGACTTGACAAAATCTCTTAATGACCTGGGACTAAG
  AGAATTATATGCGATGGATGTCAACAGAGAGTCCTGCCAAATATCACAAAACCTAGATATTATGAAGGAG
  AAAGAAAATAAAGGGTTTTTCAGTTTTTTTCAACGCAGTAAGAAAAAGCGAGACCAAACTGCAAGTGCCC
  CTGCAACCCCTCTAGTAAATAAGCACCGCCCAACTTTTACAAGGTCCAATACCATTTCCAAACCATATAT
  TTCCAACACCCTGCCGTCGGATGCACCCAAGAAGAGGCGGGCTCCACTGCCCCCGATGCCAGCATCTCAG
  AGTGTCCCCCAAGACCTTGCACACATCCAGGAGAGGCCTGCTTCTTGTATAGTGAAATCCATGAGCGTGG
  ATGAGACAGATAAGAGTCCCTGTGAAGCAGGAAGAGTGAGGGCAGGTTCACTGCAGCTCAGCAGCATGTC
  TGCAGGGAATTCATCTTTGAGAAGGACAAAGCGAAAAGCACCTTCCCCACCCTCCAAAATACCCCCGCAT
  CAAAGTGATGAAAATAGTCGTGTGACTGCCTTACAGCCAGTAGATGGAGTTCCTCCAGACAGTGCTTCAG
  AAGCAAACTCTCCTGAGGAGCTATCCAGCCCAGCTGGAATAAGCTCTGATTATAGCCTTGAAGAGATAGA
  TGAAAAGGAAGAACTGAGTGAAGTGCCTAAAGTTGAAGCTGAAAATATTTCTCCGAAGTCACAAGATATT
  CCTTTTGTATCTACTGATATAATAAATACACTGAAAAATGATCCTGACTCAGCCCTTGGCAATGGTAGTG
  GAGAGTTCTCACAAAACTCCATGGAAGAAAAACAAGAAACTAAAAGCACAGATGGACAAGAACCACACAG
  TGTAGTATATGATACAAGCAATGGAAAGAAGGTAGTTGACAGTATAAGAAACTTGAAGTCGTTGGGCCCA
  AACCAAGAGAATGTAGTTCAAAATGAAATAATTGTCTATCCAGAGAACACAGAAGACAATATGAAAAATG
  GAGTGAAGAAAACAGAAATCAATGTAGAAGGTGTTGCCAAAAATAACAACATTGATATGGAAGTTGAGAG
  ACCATCAAACTCTGAGGCACATGAAACTGATACTGCTATAAGTTACAAGGAAAACCATCTAGCAGCTTCA
  TCAGTACCAGATCAAAAACTGAATCAACCCAGTGCAGAAAAGACAAAAGATGCAGCAATTCAGACAACCC
  CTTCTTGTAACAGTTTTGATGGGAAACACCAAGATCATAATTTATCTGACTCCAAAGTTGAAGAATGTGT
  GCAAACTTCAAATAACAACATATCAACTCAACACTCATGCTTAAGTTCACAAGATTCTGTAAATACCTCA
  AGGGAATTCAGGAGTCAAGGCACCCTAATTATACATTCAGAAGATCCGCTTACCGTAAAAGATCCAATTT
  GTGCACATGGTAATGATGATCTTTTGCCTCCTGTAGATAGGATTGACAAAAATTCCACTGCTTCTTACCT
  AAAGAATTACCCACTTTATAGACAGGACTACAATCCCAAGCCAAAACCTTCAAATGAAATTACACGAGAG
  TATATACCCAAAATTGGCATGACTACTTATAAAATAGTGCCTCCCAAATCCTTGGAAATATCGAAAGACT
  GGCAATCAGAAACCATAGAGTATAAAGATGATCAGGACATGCATGCTTTAGGGAAAAAGCACACTCATGA
  GAATGTGAAAGAAACTGCCATCCAAACAGAAGATTCTGCTATTTCTGAAAGCCCAGAAGAGCCACTGCCA
  AACCTTAAACCGAAGCCTAACCTGAGAACAGAGCATCAAGTGCCCAGTTCTGTGAGCTCACCTGATGATG
  CCATGGTTAGTCCTCTGAAACCTGCTCCCAAAATGACAAGAGACACTGGCACAGCTCCTTTTGCACCAAA
  TTTGGAAGAAATAAACAATATTTTGGAATCAAAATTTAAATCTCGGGCTTCAAATGCCCAGGCCAAACCC
  AGCTCTTTTTTTTTGCAGATGCAGAAGAGAGTATCGGGTCACTATGTGACATCTGCAGCTGCCAAGAGTG
  TCCATGCTGCCCCTAATCCTGCTCCAAAAGAACTGACAAATAAAGAGGCAGAAAGGGATATGCTGCCTTC
  TCCGGAGCAGACTCTTTCTCCCTTAAGTAAAATGCCTCACTCTGTTCCACAACCCCTTGTTGAAAAAACT
  GATGATGATGTCATCGGTCAGGCTCCTGCTGAAGCCTCCCCTCCTCCCATAGCTCCAAAACCTGTGACAA
  TTCCTGCTAGTCAGGTATCCACACAAAATCTGAAGACTTTGAAAACTTTTGGTGCCCCACGACCATACTC
  AAGTTCTGGTCCTTCACCGTTTGCTCTTGCTGTAGTGAAAAGGTCACAGTCTTTCAGTAAAGAGCGCACC
  GAGTCACCTAGTGCCAGTGCATTGGTCCAACCTCCAGCCAACACAGAGGAAGGGAAGACTCATTCTGTAA
  ATAAATTTGTGGACATCCCACAGCTTGGTGTGTCTGATAAGGAAAATAACTCTGCACATAATGAACAGAA
  TTCCCAAATACCAACTCCAACTGATGGCCCATCATTCACTGTTATGAGACAAAGTTCTTTAACATTCCAA
  AGCTCTGACCCAGAACAGATGCGACAGAGTTTGCTGACTGCAATCCGTTCGGGAGAGGCTGCTGCCAAAT
  TGAAAAGGGTTACCATTCCATCAAATACAATATCTGTGAATGGAAGGTCAAGACTCAGCCATTCCATGTC
  CCCTGATGCCCAGGACGGCCAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC240024 representing NM_001278460
  Red=Cloning site Green=Tags(s)
  
  MPPSWSPLMCGRAAEAAASSRTPGREMGQAVTRRLGAGARAAPRRAMDGRTPRPQDAPARRKPKAKAPLP
  PAETKYTDVSSAADSVESTAFIMEQKENMIDKDVELSVVLPGDIIKSTTVHGSKPMMDLLIFLCAQYHLN
  PSSYTIDLLSAEQNHIKFKPNTPIGMLEVEKVILKPKMLDKKKPTPIIPEKTVRVVINFKKTQKTIVRVS
  PHASLQELAPIICSKCEFDPLHTLLLKDYQSQEPLDLTKSLNDLGLRELYAMDVNRESCQISQNLDIMKE
  KENKGFFSFFQRSKKKRDQTASAPATPLVNKHRPTFTRSNTISKPYISNTLPSDAPKKRRAPLPPMPASQ
  SVPQDLAHIQERPASCIVKSMSVDETDKSPCEAGRVRAGSLQLSSMSAGNSSLRRTKRKAPSPPSKIPPH
  QSDENSRVTALQPVDGVPPDSASEANSPEELSSPAGISSDYSLEEIDEKEELSEVPKVEAENISPKSQDI
  PFVSTDIINTLKNDPDSALGNGSGEFSQNSMEEKQETKSTDGQEPHSVVYDTSNGKKVVDSIRNLKSLGP
  NQENVVQNEIIVYPENTEDNMKNGVKKTEINVEGVAKNNNIDMEVERPSNSEAHETDTAISYKENHLAAS
  SVPDQKLNQPSAEKTKDAAIQTTPSCNSFDGKHQDHNLSDSKVEECVQTSNNNISTQHSCLSSQDSVNTS
  REFRSQGTLIIHSEDPLTVKDPICAHGNDDLLPPVDRIDKNSTASYLKNYPLYRQDYNPKPKPSNEITRE
  YIPKIGMTTYKIVPPKSLEISKDWQSETIEYKDDQDMHALGKKHTHENVKETAIQTEDSAISESPEEPLP
  NLKPKPNLRTEHQVPSSVSSPDDAMVSPLKPAPKMTRDTGTAPFAPNLEEINNILESKFKSRASNAQAKP
  SSFFLQMQKRVSGHYVTSAAAKSVHAAPNPAPKELTNKEAERDMLPSPEQTLSPLSKMPHSVPQPLVEKT
  DDDVIGQAPAEASPPPIAPKPVTIPASQVSTQNLKTLKTFGAPRPYSSSGPSPFALAVVKRSQSFSKERT
  ESPSASALVQPPANTEEGKTHSVNKFVDIPQLGVSDKENNSAHNEQNSQIPTPTDGPSFTVMRQSSLTFQ
  SSDPEQMRQSLLTAIRSGEAAAKLKRVTIPSNTISVNGRSRLSHSMSPDAQDGH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001278460
• ORF Size: 3522 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001278460.2
• RefSeq Size: 9414 bp
• RefSeq ORF: 3525 bp
• Locus ID: 22837
• UniProt ID: Q53SF7
• Cytogenetics: 2q24.3
• MW: 129.1 kDa