RPS6KC1 (NM_001287221) Human Tagged ORF Clone

CAT#: RC239734

RPS6KC1 (myc-DDK-tagged) - Human ribosomal protein S6 kinase, 52kDa, polypeptide 1 (RPS6KC1), transcript variant 6

Product Images

Circular map for RC239734

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RPS6KC1
• Synonyms: humS6PKh1; RPK118; RSKL1; S6K-delta-1; S6PKh1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC239734 representing NM_001287221
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTCCAATCAAAATTCTCCCATTAGAACTTTTGGTCTCAATCTTTCTTCGGATTCTTCAGCACTAG
  GGGCTGTTGCTTCTGACAGTGAACAGAGCAAAACAGAAGAAGAACGGGAAAGTCGTAGCCTCTTTCCTGG
  CAGTTTAAAGCCGAAGCTTGGCAAGAGAGATTATTTGGAGAAAGCAGGAGAATTAATAAAGCTGGCTTTA
  AAAAAGGAAGAAGAAGACGACTATGAAGCTGCTTCTGATTTTTATAGGAAGGGAGTTGATTTACTCCTAG
  AAGGTGTTCAAGGAGAGTCAAGCCCTACCCGTCGAGAAGCTGTGAAGAGAAGAACAGCCGAGTACCTCAT
  GCGGGCAGAAAGTATCTCTAGTCTTTATGGGAAACCTCAGCTTGATGATGTATCTCAGCCTCCAGGATCA
  CTAAGTTCAAGGCCCCTTTGGAACCTAAGGAGCCCTGCCGAGGAGCTGAAGGCCTTCAGAGTCCTTGGGG
  TGATTGACAAGGTTTTACTTGTAATGGACACAAGGACAGAACAGACTTTCATTTTAAAAGGTCTAAGGAA
  AAGCAGTGAATACAGCAGGAACAGAAAGACCATCATCCCCCGCTGTGTGCCCAACATGGTGTGTCTGCAT
  AAGTACATCATCTCTGAGGAGTCAGTATTTCTTGTGCTGCAGCATGCGGAAGGTGGCAAACTGTGGTCAT
  ATATCAGTAAATTTCTAAACAGAAGTCCTGAAGAAAGCTTTGACATCAAGGAAGTGAAAAAACCTACACT
  TGCAAAAGTTCACCTGCAGCAGCCAACTTCTAGTCCTCAGGACAGCAGTAGCTTTGAATCCAGAGGAAGT
  GATGGTGGAAGCATGCTTAAAGCTCTGCCTTTGAAGAGTAGTCTTACTCCAAGTTCTCAAGATGACAGCA
  ACCAGGAAGATGATGGCCAAGATAGCTCTCCAAAGTGGCCAGATTCTGGTTCAAGTTCAGAAGAAGAATG
  TACTACTAGTTATTTAACATTATGCAATGAATATGGGCAAGAAAAGATTGAACCAGGGTCTTTGAATGAG
  GAGCCCTTCATGAAGACTGAAGGGAATGGTGTTGATACAAAAGCTATTAAAAGCTTCCCAGCACACCTTG
  CTGCTGACAGTGACAGCCCCAGCACACAGCTGAGAGCTCACGAGCTGAAGTTCTTCCCCAACGATGACCC
  AGAAGCAGTTAGTTCTCCAAGAACATCAGATTCCCTCAGTAGATCAAAAAATAGCCCCATGGAATTCTTT
  AGGATAGACAGTAAGGATAGCGCAAGTGAACTCCTGGGACTTGACTTTGGAGAAAAATTGTATAGTCTAA
  AATCAGAACCTTTGAAACCATTCTTTACTCTTCCAGATGGAGACAGTGCTTCTAGGAGTTTTAATACTAG
  TGAAAGCAAGGTAGAGTTTAAAGCTCAGGACACCATTAGCAGGGGCTCAGATGACTCAGTGCCAGTTATT
  TCATTTAAAGATGCTGCTTTTGATGATGTCAGTGGTACTGATGAAGGAAGACCTGATCTTCTTGTAAATT
  TACCTGGTGAATTGGAGTCAACAAGAGAAGCTGCAGCAATGGGACCTACTAAGTTTACACAAACTAATAT
  AGGGATAATAGAAAATAAACTCTTGGAAGCCCCTGATGTTTTATGCCTCAGGCTTAGTACTGAACAATGC
  CAAGCACATGAGGAGAAAGGCATAGAGGAACTGAGTGATCCCTCTGGGCCCAAATCCTATAGTATAACAG
  AGAAACACTATGCACAGGAGGATCCCAGGATGTTATTTGTAGCAGCTGTTGATCATAGTAGTTCAGGAGA
  TATGTCTTTGTTACCCAGCTCAGATCCTAAGTTTCAAGGACTTGGAGTGGTTGAGTCAGCAGTAACTGCA
  AACAACACAGAAGAAAGCTTATTCCGTATTTGTAGTCCACTCTCAGGTGCTAATGAATATATTGCAAGCA
  CAGACACTTTAAAAACAGAAGAAGTATTGCTGTTTACAGATCAGACTGATGATTTGGCTAAAGAGGAACC
  AACTTCTTTATTCCAGAGAGACTCTGAGACTAAGGGTGAAAGTGGTTTAGTGCTAGAAGGAGACAAGGAA
  ATACATCAGATTTTTGAGGACCTTGATAAAAAATTAGCACTAGCCTCCAGGTTTTACATCCCAGAGGGCT
  GCATTCAAAGATGGGCAGCTGAAATGGTGGTAGCCCTTGATGCTTTACATAGAGAGGGAATTGTGTGCCG
  CGATTTGAACCCAAACAACATCTTATTGAATGATAGAGGACACATTCAGCTAACGTATTTTAGCAGGTGG
  AGTGAGGTTGAAGATTCCTGTGACAGCGATGCCATAGAGAGAATGTACTGTGCCCCAGAGGTTGGAGCAA
  TCACTGAAGAAACTGAAGCCTGTGATTGGTGGAGTTTGGGTGCTGTCCTCTTTGAACTTCTCACTGGCAA
  GACTCTGGTTGAATGCCATCCAGCAGGAATAAATACTCACACTACTTTGAACATGCCAGAATGTGTCTCT
  GAAGAGGCTCGCTCACTCATTCAACAGCTCTTGCAGTTCAATCCTCTGGAACGACTTGGTGCTGGAGTTG
  CTGGTGTTGAAGATATCAAATCTCATCCATTTTTTACCCCTGTGGATTGGGCAGAACTGATGAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC239734 representing NM_001287221
  Red=Cloning site Green=Tags(s)
  
  MASNQNSPIRTFGLNLSSDSSALGAVASDSEQSKTEEERESRSLFPGSLKPKLGKRDYLEKAGELIKLAL
  KKEEEDDYEAASDFYRKGVDLLLEGVQGESSPTRREAVKRRTAEYLMRAESISSLYGKPQLDDVSQPPGS
  LSSRPLWNLRSPAEELKAFRVLGVIDKVLLVMDTRTEQTFILKGLRKSSEYSRNRKTIIPRCVPNMVCLH
  KYIISEESVFLVLQHAEGGKLWSYISKFLNRSPEESFDIKEVKKPTLAKVHLQQPTSSPQDSSSFESRGS
  DGGSMLKALPLKSSLTPSSQDDSNQEDDGQDSSPKWPDSGSSSEEECTTSYLTLCNEYGQEKIEPGSLNE
  EPFMKTEGNGVDTKAIKSFPAHLAADSDSPSTQLRAHELKFFPNDDPEAVSSPRTSDSLSRSKNSPMEFF
  RIDSKDSASELLGLDFGEKLYSLKSEPLKPFFTLPDGDSASRSFNTSESKVEFKAQDTISRGSDDSVPVI
  SFKDAAFDDVSGTDEGRPDLLVNLPGELESTREAAAMGPTKFTQTNIGIIENKLLEAPDVLCLRLSTEQC
  QAHEEKGIEELSDPSGPKSYSITEKHYAQEDPRMLFVAAVDHSSSGDMSLLPSSDPKFQGLGVVESAVTA
  NNTEESLFRICSPLSGANEYIASTDTLKTEEVLLFTDQTDDLAKEEPTSLFQRDSETKGESGLVLEGDKE
  IHQIFEDLDKKLALASRFYIPEGCIQRWAAEMVVALDALHREGIVCRDLNPNNILLNDRGHIQLTYFSRW
  SEVEDSCDSDAIERMYCAPEVGAITEETEACDWWSLGAVLFELLTGKTLVECHPAGINTHTTLNMPECVS
  EEARSLIQQLLQFNPLERLGAGVAGVEDIKSHPFFTPVDWAELMR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001287221
• ORF Size: 2655 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001287221.2
• RefSeq Size: 4098 bp
• RefSeq ORF: 2658 bp
• Locus ID: 26750
• Cytogenetics: 1q32.3
• Protein Families: Druggable Genome, Protein Kinase
• MW: 98.3 kDa
• Gene Summary: Sphingosine kinase catalyzes the formation of sphingosine 1 phosphate, a lipid cellular messenger. The protein encoded by this gene can bind to sphingosine kinase and to phosphatidylinositol 3-phosphate, suggesting a role in sphingosine 1 phophate signaling. The encoded protein can also bind to peroxiredoxin-3 and may help transport it to mitochondria. [provided by RefSeq, Mar 2017]