PHF17 (JADE1) (NM_001287439) Human Tagged ORF Clone
CAT#: RC239666
- TrueORF®
JADE1 (myc-DDK-tagged) - Human jade family PHD finger 1 (JADE1), transcript variant 4
ORF Plasmid: tGFP
"NM_001287439" in other vectors (2)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | PHF17 |
Synonyms | PHF17 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC239666 representing NM_001287439
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAACGAGGTCGCCTTCCCAGCAGCAGTGAGGATTCTGACGACAATGGCAGCCTGTCAACTACTTGGT CCCAGAATTCCCGATCCCAGCATAGGAGAAGCTCCTGCTCCAGACATGAAGATCGAAAGCCTTCAGAGGT GTTTAGGACAGACCTGATCACTGCCATGAAGTTGCATGACTCCTACCAGCTGAATCCGGATGAGTACTAT GTGTTGGCAGATCCCTGGAGACAGGAATGGGAGAAAGGGGTCCAGGTGCCTGTGAGCCCGGGGACCATCC CTCAGCCTGTGGCCAGGGTTGTGTCTGAAGAGAAATCCCTCATGTTCATCAGGCCCAAGAAGTACATCGT GTCATCAGGCTCTGAGCCTCCCGAGTTGGGCTATGTGGACATCCGGACGCTGGCTGACAGCGTGTGTCGC TATGACCTCAATGACATGGATGCTGCATGGCTGGAACTGACCAATGAAGAATTTAAGGAGATGGGAATGC CTGAACTAGATGAATACACCATGGAGAGGGTCCTAGAGGAATTTGAGCAGCGATGCTACGACAATATGAA TCATGCCATAGAGACTGAGGAAGGCCTGGGGATCGAATATGATGAAGATGTTGTCTGTGATGTCTGCCAG TCTCCTGATGGTGAGGACGGCAATGAGATGGTGTTCTGTGACAAATGCAACATCTGTGTGCACCAGGCCT GTTATGGAATCCTCAAGGTACCAGAGGGCAGCTGGCTGTGCCGGACATGTGCCCTGGGGGTTCAGCCAAA ATGTCTGCTGTGTCCGAAGAAGGGTGGAGCTATGAAGCCCACCCGTAGCGGAACCAAGTGGGTCCACGTT AGCTGTGCTCTGTGGATCCCTGAGGTGAGCATTGGCAGCCCAGAGAAGATGGAGCCCATCACCAAGGTGT CACACATTCCCAGCAGCCGGTGGGCGCTAGTGTGCAGCCTCTGCAATGAGAAGTTTGGGGCCTCTATACA GTGCTCTGTGAAGAACTGCCGCACAGCCTTCCATGTGACCTGTGCTTTTGACCGGGGCCTGGAGATGAAG ACCATCTTAGCAGAGAATGATGAAGTCAAGTTCAAGTCCTATTGCCCAAAGCACAGCTCACATAGGAAAC CCGAGGAGAGTCTTGGCAAGGGGGCTGCACAGGAGAATGGGGCCCCTGAGTGTTCCCCCCGGAATCCGCT GGAGCCCTTTGCCAGCCTTGAGCAGAACCGGGAGGAGGCCCACCGGGTGAGTGTCCGTAAGCAGAAGCTG CAGCAGTTGGAGGATGAGTTCTACACCTTCGTCAACCTGCTGGATGTTGCCAGGGCTCTGCGGCTGCCTG AGGAAGTAGTGGATTTCCTGTACCAGTACTGGAAGTTGAAGAGGAAGGTCAACTTCAACAAGCCCCTGAT CACCCCAAAGAAAGATGAAGAGGACAATCTAGCCAAGCGGGAGCAGGATGTCTTATTTAGGAGGCTGCAG CTGTTCACGCACCTGCGGCAGGACCTGGAGAGGGTTCGGAACCTCACTTACATGGTGACCCGCAGGGAAA AGATTAAACGGTCTGTGTGCAAAGTCCAGGAACAGATATTCAATCTTTACACTAAGCTTTTGGAGCAAGA AAGAGTTTCAGGTGTGCCTTCTTCCTGCTCCTCCTCCTCACTGGAAAACATGCTTTTGTTTAACAGTCCT TCTGTTGGCCCTGATGCTCCCAAGATAGAGGACTTGAAGTGGCATTCTGCATTCTTCAGAAAACAAATGG GTACTTCCTTGGTTCATTCGCTGAAAAAGCCCCATAAGCGAGATCCTTTGCAGAATAGCCCTGGAAGTGA AGGCAAAACCCTGCTGAAGCAGCCAGACCTGTGTGGTAGAAGGGAGGGGATGGTGGTCCCAGAGAGCTTT TTGGGTTTAGAAAAGACCTTTGCAGAAGCACGTCTCATATCAGCACAACAGAAAAATGGTGTGGTGATGC CAGACCATGGGAAAAGAAGAGACAATCGTTTTCATTGTGATCTCATTAAAGGAGACTTAAAGGACAAATC TTTTAAACAGAGTCACAAGCCTCTCAGGTCCACAGATGTGTCCCAGAGGCATCTGGACAACACAAGAGCT GCCACCTCCCCTGGAGTGGGGCAGTCAGCACCTGGCACAAGGAAGGAGATAGTGCCCAAGTGCAATGGCT CCCTAATCAAAGTAAACTATAATCAGACTGCAGTCAAAGTGCCTACAACACCTGCCAGCCCAGTGAAAAA CTGGGGAGGATTCCGGATTCCAAAGAAGGGGGAACGGCAGCAGCAGGGAGAGGCCCACGATGGGGCCTGC CACCAGCACTCAGACTACCCATATTTGGGCTTAGGCCGAGTTCCAGCCAAGGAAAGGGCAAAAAGCAAAT TAAAATCCGACAATGAGAATGACGGGTATGTCCCCGATGTGGAAATGAGTGACTCAGAGAGTGAGGCATC AGAAAAGAAATGTATACACACCAGCAGCACTATCAGCAGAAGGACAGACATTATCAGGAGAAGCATCTTG GCTTCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC239666 representing NM_001287439
Red=Cloning site Green=Tags(s) MKRGRLPSSSEDSDDNGSLSTTWSQNSRSQHRRSSCSRHEDRKPSEVFRTDLITAMKLHDSYQLNPDEYY VLADPWRQEWEKGVQVPVSPGTIPQPVARVVSEEKSLMFIRPKKYIVSSGSEPPELGYVDIRTLADSVCR YDLNDMDAAWLELTNEEFKEMGMPELDEYTMERVLEEFEQRCYDNMNHAIETEEGLGIEYDEDVVCDVCQ SPDGEDGNEMVFCDKCNICVHQACYGILKVPEGSWLCRTCALGVQPKCLLCPKKGGAMKPTRSGTKWVHV SCALWIPEVSIGSPEKMEPITKVSHIPSSRWALVCSLCNEKFGASIQCSVKNCRTAFHVTCAFDRGLEMK TILAENDEVKFKSYCPKHSSHRKPEESLGKGAAQENGAPECSPRNPLEPFASLEQNREEAHRVSVRKQKL QQLEDEFYTFVNLLDVARALRLPEEVVDFLYQYWKLKRKVNFNKPLITPKKDEEDNLAKREQDVLFRRLQ LFTHLRQDLERVRNLTYMVTRREKIKRSVCKVQEQIFNLYTKLLEQERVSGVPSSCSSSSLENMLLFNSP SVGPDAPKIEDLKWHSAFFRKQMGTSLVHSLKKPHKRDPLQNSPGSEGKTLLKQPDLCGRREGMVVPESF LGLEKTFAEARLISAQQKNGVVMPDHGKRRDNRFHCDLIKGDLKDKSFKQSHKPLRSTDVSQRHLDNTRA ATSPGVGQSAPGTRKEIVPKCNGSLIKVNYNQTAVKVPTTPASPVKNWGGFRIPKKGERQQQGEAHDGAC HQHSDYPYLGLGRVPAKERAKSKLKSDNENDGYVPDVEMSDSESEASEKKCIHTSSTISRRTDIIRRSIL AS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001287439 |
ORF Size | 2526 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001287439.2 |
RefSeq Size | 5890 bp |
RefSeq ORF | 2529 bp |
Locus ID | 79960 |
UniProt ID | Q6IE81 |
Cytogenetics | 4q28.2 |
Protein Families | Druggable Genome, Transcription Factors |
MW | 96 kDa |
Gene Summary | Component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. Transcriptional coactivator, it may also promote acetylation of nucleosomal histone H4 by KAT5. Promotes apoptosis. May act as a renal tumor suppressor. Negatively regulates canonical Wnt signaling; at least in part, cooperates with NPHP4 in this function.[UniProtKB/Swiss-Prot Function] |
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