MPP2 (NM_001278372) Human Tagged ORF Clone

CAT#: RC238957

MPP2 (myc-DDK-tagged) - Human membrane protein, palmitoylated 2 (MAGUK p55 subfamily member 2) (MPP2), transcript variant 4

Product Images

Circular map for RC238957

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MPP2
• Synonyms: DLG2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC238957 representing NM_001278372
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGGTTGCCGCCACCAACTCTGAAACTGCCATGCAGCAAGTCCTGGACAACTTGGGATCCCTCCCCA
  GTGCCACGGGGGCTGCAGAGCTGGACCTGATCTTCCTTCGAGGCATTATGGAAAGTCCCATAGTAAGATC
  CCTGGCCAAGGTGATAATGGTATTGTGGTTTATGCAGCAGAATGTCTTTGTTCCTATGAAATACATGCTG
  AAATACTTTGGGGCCCATGAGAGGCTGGAGGAGACGAAGCTGGAGGCCGTGAGAGACAACAACCTGGAGC
  TGGTGCAGGAGATCCTGCGGGACCTGGCGCAGCTGGCTGAGCAGAGCAGCACAGCCGCCGAGCTGGCCCA
  CATCCTCCAGGAGCCCCACTTCCAGTCCCTCCTGGAGACGCACGACTCTGTGGCCTCAAAGACCTATGAG
  ACACCACCCCCCAGCCCTGGCCTGGACCCTACATTCAGCAACCAGCCTGTACCTCCCGATGCTGTGCGCA
  TGGTGGGCATCCGCAAGACAGCCGGAGAACATCTGGGTGTAACGTTCCGCGTGGAGGGCGGCGAGCTGGT
  GATCGCGCGCATTCTGCATGGGGGCATGGTGGCTCAACAAGGCCTGCTGCATGTGGGTGACATCATCAAG
  GAGGTGAACGGGCAGCCAGTGGGCAGTGACCCCCGCGCACTGCAGGAGCTCCTGCGCAATGCCAGTGGCA
  GTGTCATCCTCAAGATCCTGCCCAGCTACCAGGAGCCCCATCTGCCCCGCCAGGTATTTGTGAAATGTCA
  CTTTGACTATGACCCGGCCCGAGACAGCCTCATCCCCTGCAAGGAAGCAGGCCTGCGCTTCAACGCCGGG
  GACTTGCTCCAGATCGTAAACCAGGATGATGCCAACTGGTGGCAGGCATGCCATGTCGAAGGGGGCAGTG
  CTGGGCTCATTCCCAGCCAGCTGCTGGAGGAGAAGCGGAAAGCATTTGTCAAGAGGGACCTGGAGCTGAC
  ACCAAACTCAGGGACCCTATGCGGCAGCCTTTCAGGAAAGAAAAAGAAGCGAATGATGTATTTGACCACC
  AAGAATGCAGAGTTTGACCGTCATGAGCTGCTCATTTATGAGGAGGTGGCCCGCATGCCCCCGTTCCGCC
  GGAAAACCCTGGTACTGATTGGGGCTCAGGGCGTGGGACGGCGCAGCCTGAAGAACAAGCTCATCATGTG
  GGATCCAGATCGCTATGGCACCACGGTGCCCTACACCTCCCGGCGGCCGAAAGACTCAGAGCGGGAAGGT
  CAGGGTTACAGCTTTGTGTCCCGTGGGGAGATGGAGGCTGACGTCCGTGCTGGGCGCTACCTGGAGCATG
  GCGAATACGAGGGCAACCTGTATGGCACACGTATTGACTCCATCCGGGGCGTGGTCGCTGCTGGGAAGGT
  GTGCGTGCTGGATGTCAACCCCCAGGCGGTGAAGGTGCTACGAACGGCCGAGTTTGTCCCTTACGTGGTG
  TTCATCGAGGCCCCAGACTTCGAGACCCTGCGGGCCATGAACAGGGCTGCGCTGGAGAGTGGAATATCCA
  CCAAGCAGCTCACGGAGGCGGACCTGAGACGGACAGTGGAGGAGAGCAGCCGCATCCAGCGGGGCTACGG
  GCACTACTTTGACCTCTGCCTGGTCAATAGCAACCTGGAGAGGACCTTCCGCGAGCTCCAGACAGCCATG
  GAGAAGCTACGGACAGAGCCCCAGTGGGTGCCTGTCAGCTGGGTGTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC238957 representing NM_001278372
  Red=Cloning site Green=Tags(s)
  
  MPVAATNSETAMQQVLDNLGSLPSATGAAELDLIFLRGIMESPIVRSLAKVIMVLWFMQQNVFVPMKYML
  KYFGAHERLEETKLEAVRDNNLELVQEILRDLAQLAEQSSTAAELAHILQEPHFQSLLETHDSVASKTYE
  TPPPSPGLDPTFSNQPVPPDAVRMVGIRKTAGEHLGVTFRVEGGELVIARILHGGMVAQQGLLHVGDIIK
  EVNGQPVGSDPRALQELLRNASGSVILKILPSYQEPHLPRQVFVKCHFDYDPARDSLIPCKEAGLRFNAG
  DLLQIVNQDDANWWQACHVEGGSAGLIPSQLLEEKRKAFVKRDLELTPNSGTLCGSLSGKKKKRMMYLTT
  KNAEFDRHELLIYEEVARMPPFRRKTLVLIGAQGVGRRSLKNKLIMWDPDRYGTTVPYTSRRPKDSEREG
  QGYSFVSRGEMEADVRAGRYLEHGEYEGNLYGTRIDSIRGVVAAGKVCVLDVNPQAVKVLRTAEFVPYVV
  FIEAPDFETLRAMNRAALESGISTKQLTEADLRRTVEESSRIQRGYGHYFDLCLVNSNLERTFRELQTAM
  EKLRTEPQWVPVSWVY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001278372
• ORF Size: 1728 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001278372.2
• RefSeq Size: 4717 bp
• RefSeq ORF: 1731 bp
• Locus ID: 4355
• UniProt ID: Q14168
• Cytogenetics: 17q21.31
• Protein Families: Druggable Genome
• MW: 65 kDa
• Gene Summary: Palmitoylated membrane protein 2 is a member of a family of membrane-associated proteins termed MAGUKs (membrane-associated guanylate kinase homologs). MAGUKs interact with the cytoskeleton and regulate cell proliferation, signaling pathways, and intracellular junctions. Palmitoylated membrane protein 2 contains a conserved sequence, called the SH3 (src homology 3) motif, found in several other proteins that associate with the cytoskeleton and are suspected to play important roles in signal transduction. [provided by RefSeq, Jul 2008]