PTPN5 (NM_001278238) Human Tagged ORF Clone

CAT#: RC238809

PTPN5 (myc-DDK-tagged) - Human protein tyrosine phosphatase, non-receptor type 5 (striatum-enriched) (PTPN5), transcript variant 5

Product Images

Circular map for RC238809

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PTPN5
• Synonyms: PTPSTEP; STEP; STEP61
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC238809 representing NM_001278238
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGCTGCAGTGAGAGGCTACCGGGTCTCCCCCAGCCGATAGTGATGGAGGCACTGGACGAGGCTGAAG
  GGCTCCAGGACTCACAGAGAGAGATGCCGCCACCCCCTCCTCCCTCGCCGCCCTCAGATCCAGCTCAGAA
  GCCACCACCTCGAGGCGCTGGGAGCCACTCCCTCACTGTCAGGAGCAGCCTGTGCCTGTTCGCTGCCTCA
  CAGTTCCTGCTTGCCTGTGGGGTGCTCTGGTTCAGCGGTTATGGCCACATCTGGTCACAGAACGCCACAA
  ACCTCGTCTCCTCTTTGCTGACGCTCCTGAAACAGCTGGAACCCACGGCCTGGCTTGACTCTGGGACGTG
  GGGAGTCCCCAGTCTGCTGCTGGTCTTTCTGTCCGTGGGCCTGGTCCTCGTTACCACCCTGGTGTGGCAC
  CTCCTGAGGACACCCCCAGAGCCACCCACCCCACTGCCCCCTGAGGACAGGCGCCAGTCAGTGAGCCGCC
  AGCCCTCCTTCACCTACTCAGAGTGGATGGAGGAGAAGATCGAGGATGACTTCCTGGACCTCGACCCGGT
  GCCCGAGACTCCTGTGTTTGATTGTGTGATGGACATCAAGCCTGAGGCTGACCCCACCTCACTCACCGTC
  AAGTCCATGGGTCTGCAGGAGAGGAGGGGTTCCAATGTCTCCCTGACCCTGGACATGTGCACTCCGGGCT
  GCAACGAGGAGGGCTTTGGCTATCTCATGTCCCCACGTGAGGAGTCCGCCCGCGAGTACCTGCTCAGCGC
  CTCCCGTGTCCTCCAAGCAGAAGAGCTTCATGAAAAGGCCCTGGACCCTTTCCTGCTGCAGGCGGAATTC
  TTTGAAATCCCCATGAACTTTGTGGATCCGAAAGAGTACGACATCCCTGGGCTGGTGCGGAAGAACCGGT
  ACAAAACCATACTTCCCAACCCTCACAGCAGAGTGTGTCTGACCTCACCAGACCCTGACGACCCTCTGAG
  TTCCTACATCAATGCCAACTACATCCGGGGCTATGGTGGGGAGGAGAAGGTGTACATCGCCACTCAGGGA
  CCCATCGTCAGCACGGTCGCCGACTTCTGGCGCATGGTGTGGCAGGAGCACACGCCCATCATTGTCATGA
  TCACCAACATCGAGGAGATGAACGAGAAATGCACCGAGTATTGGCCGGAGGAGCAGGTGGCGTACGACGG
  TGTTGAGATCACTGTGCAGAAAGTCATTCACACGGAGGATTACCGGCTGCGACTCATCTCCCTCAAGAGT
  GGGACTGAGGAGCGAGGCCTGAAGCATTACTGGTTCACATCCTGGCCCGACCAGAAGACCCCAGACCGGG
  CCCCCCCACTCCTGCACCTGGTGCGGGAGGTGGAGGAGGCAGCCCAGCAGGAGGGGCCCCACTGTGCCCC
  CATCATCGTCCACTGCAGTGCAGGGATTGGGAGGACCGGCTGCTTCATTGCCACCAGCATCTGCTGCCAG
  CAGCTGCGGCAGGAGGGTGTGGTGGACATCCTGAAGACCACGTGCCAGCTCCGTCAGGACAGGGGCGGCA
  TGATCCAGACATGCGAGCAGTACCAGTTTGTGCACCACGTCATGAGCCTCTACGAAAAGCAGCTGTCCCA
  CCAGTCCCCAGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC238809 representing NM_001278238
  Red=Cloning site Green=Tags(s)
  
  MCCSERLPGLPQPIVMEALDEAEGLQDSQREMPPPPPPSPPSDPAQKPPPRGAGSHSLTVRSSLCLFAAS
  QFLLACGVLWFSGYGHIWSQNATNLVSSLLTLLKQLEPTAWLDSGTWGVPSLLLVFLSVGLVLVTTLVWH
  LLRTPPEPPTPLPPEDRRQSVSRQPSFTYSEWMEEKIEDDFLDLDPVPETPVFDCVMDIKPEADPTSLTV
  KSMGLQERRGSNVSLTLDMCTPGCNEEGFGYLMSPREESAREYLLSASRVLQAEELHEKALDPFLLQAEF
  FEIPMNFVDPKEYDIPGLVRKNRYKTILPNPHSRVCLTSPDPDDPLSSYINANYIRGYGGEEKVYIATQG
  PIVSTVADFWRMVWQEHTPIIVMITNIEEMNEKCTEYWPEEQVAYDGVEITVQKVIHTEDYRLRLISLKS
  GTEERGLKHYWFTSWPDQKTPDRAPPLLHLVREVEEAAQQEGPHCAPIIVHCSAGIGRTGCFIATSICCQ
  QLRQEGVVDILKTTCQLRQDRGGMIQTCEQYQFVHHVMSLYEKQLSHQSPE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001278238
• ORF Size: 1623 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001278238.2
• RefSeq Size: 3068 bp
• RefSeq ORF: 1626 bp
• Locus ID: 84867
• UniProt ID: P54829
• Cytogenetics: 11p15.1
• Protein Families: Druggable Genome, Phosphatase, Transmembrane
• Protein Pathways: MAPK signaling pathway
• MW: 61.4 kDa
• Gene Summary: May regulate the activity of several effector molecules involved in synaptic plasticity and neuronal cell survival, including MAPKs, Src family kinases and NMDA receptors.[UniProtKB/Swiss-Prot Function]