TCP1 theta (CCT8) (NM_001282909) Human Tagged ORF Clone

CAT#: RC238443

CCT8 (myc-DDK-tagged) - Human chaperonin containing TCP1, subunit 8 (theta) (CCT8), transcript variant 4

Product Images

Circular map for RC238443

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TCP1 theta
• Synonyms: C21orf112; Cctq; D21S246; PRED71
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC238443 representing NM_001282909
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACCAAATGGTACAGCATCCTGCTGCAAAAATGATTGTAATGGCTTCTCATATGCAAGAGCAAGAAG
  TTGGAGATGGCACAAACTTTGTTCTGGTATTTGCTGGAGCTCTCCTGGAATTAGCTGAAGAACTTCTGAG
  GATTGGCCTGTCAGTTTCAGAGGTCATAGAAGGTTATGAAATAGCCTGCAGAAAAGCTCATGAGATTCTT
  CCTAATTTGGTATGTTGTTCTGCAAAAAACCTTCGAGATATTGATGAAGTCTCATCTCTACTTCGTACCT
  CCATAATGAGTAAACAATATGGTAATGAAGTATTTCTGGCCAAGCTTATTGCTCAGGCATGCGTATCTAT
  TTTTCCTGATTCCGGCCATTTCAATGTTGATAACATCAGAGTTTGTAAAATTCTGGGCTCTGGTATCAGT
  TCCTCTTCAGTATTGCATGGCATGGTTTTTAAGAAGGAAACCGAAGGTGATGTAACATCTGTCAAAGATG
  CAAAAATAGCAGTGTACTCTTGTCCTTTTGATGGCATGATAACAGAAACTAAGGGAACAGTGTTGATAAA
  GACTGCTGAAGAATTGATGAATTTTAGTAAGGGAGAAGAAAACCTCATGGATGCACAAGTCAAAGCTATT
  GCTGATACTGGTGCAAATGTCGTAGTAACAGGTGGCAAAGTGGCAGACATGGCTCTTCATTATGCAAATA
  AATATAATATCATGTTAGTGAGGCTAAACTCAAAATGGGATCTCCGAAGACTTTGTAAAACTGTTGGTGC
  TACAGCTCTTCCTAGATTGACACCTCCTGTCCTTGAAGAAATGGGACACTGTGACAGTGTTTACCTCTCA
  GAAGTTGGAGATACTCAGGTGGTGGTTTTTAAGCATGAAAAGGAAGATGGCGCCATTTCTACCATAGTAC
  TTCGAGGCTCTACAGACAATCTGATGGATGACATAGAAAGGGCAGTAGACGATGGTGTTAATACTTTCAA
  AGTTCTTACAAGGGATAAACGTCTTGTACCCGGAGGTGGAGCAACAGAAATTGAATTAGCCAAACAGATC
  ACATCATATGGAGAGACATGTCCTGGACTTGAACAGTATGCTATTAAGAAGTTTGCTGAGGCATTTGAAG
  CTATTCCCCGCGCACTGGCAGAAAACTCTGGAGTTAAGGCCAATGAAGTAATCTCTAAACTTTATGCAGT
  ACATCAAGAAGGAAATAAAAACGTTGGATTAGATATTGAGGCTGAAGTCCCTGCTGTAAAGGACATGCTG
  GAAGCTGGTATTCTAGATACTTACCTGGGAAAATATTGGGCTATCAAACTCGCTACTAATGCTGCAGTCA
  CTGTACTTAGAGTGGATCAGATCATCATGGCAAAACCAGCTGGTGGGCCCAAGCCTCCAAGTGGGAAGAA
  AGACTGGGATGATGACCAAAATGAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC238443 representing NM_001282909
  Red=Cloning site Green=Tags(s)
  
  MDQMVQHPAAKMIVMASHMQEQEVGDGTNFVLVFAGALLELAEELLRIGLSVSEVIEGYEIACRKAHEIL
  PNLVCCSAKNLRDIDEVSSLLRTSIMSKQYGNEVFLAKLIAQACVSIFPDSGHFNVDNIRVCKILGSGIS
  SSSVLHGMVFKKETEGDVTSVKDAKIAVYSCPFDGMITETKGTVLIKTAEELMNFSKGEENLMDAQVKAI
  ADTGANVVVTGGKVADMALHYANKYNIMLVRLNSKWDLRRLCKTVGATALPRLTPPVLEEMGHCDSVYLS
  EVGDTQVVVFKHEKEDGAISTIVLRGSTDNLMDDIERAVDDGVNTFKVLTRDKRLVPGGGATEIELAKQI
  TSYGETCPGLEQYAIKKFAEAFEAIPRALAENSGVKANEVISKLYAVHQEGNKNVGLDIEAEVPAVKDML
  EAGILDTYLGKYWAIKLATNAAVTVLRVDQIIMAKPAGGPKPPSGKKDWDDDQND
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001282909
• ORF Size: 1425 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001282909.1, NP_001269838.1
• RefSeq Size: 1927 bp
• RefSeq ORF: 1428 bp
• Locus ID: 10694
• UniProt ID: P50990
• Cytogenetics: 21q21.3
• Protein Families: Druggable Genome
• MW: 52 kDa
• Gene Summary: This gene encodes the theta subunit of the CCT chaperonin, which is abundant in the eukaryotic cytosol and may be involved in the transport and assembly of newly synthesized proteins. Alternative splicing results in multiple transcript variants of this gene. A pseudogene related to this gene is located on chromosome 1. [provided by RefSeq, Sep 2013]