PCYOX1L (NM_001301057) Human Tagged ORF Clone

CAT#: RC238112

PCYOX1L (myc-DDK-tagged) - Human prenylcysteine oxidase 1 like (PCYOX1L), transcript variant 3

Product Images

Circular map for RC238112

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PCYOX1L
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC238112 representing NM_001301057
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCCGCCGGGCAAAATCGGTGCGGGAAGGACGCGCGGTGGTTGGGGCTGGGATTGGGGGCTCTGCTG
  TGGCCCATTTTCTCCAGCAGCACTTTGGACCTCGGGTGCAGATCGACGTGTACGAGAAGGGAACCGTGGG
  TGGCCGCTTGGCCACCATCTCAGTCAACAAGCAGCACTATGAGAGCGGGGCTGCCTCCTTCCACTCCCTG
  AGCCTGCACATGCAGGACTTCGTCAAGCTGCTGGGGCTGAGGCACCGGCGCGAGGTGGTGGGCAGGAGCG
  CCATCTTCGGCGGGGAGCACTTCATGCTGGAGGAGACTGACTGGTACCTGCTGAACCTCTTCCGCCTCTG
  GTGGCACTATGGCATCAGCTTCCTGAGGCTGCAGATGTGGGTGGAGGAGGTCATGGAGAAGTTCATGAGC
  TATGGCCAGTCAGCAGCGATGCCCGCCTTTGCAGGAGCCATGTCACTAGCCGGGGCCCAAGGCAGCCTGT
  GGTCTGTGGAAGGAGGCAATAAGCTGGTTTGTTCCGGTTTGCTGAAGCTCACCAAGGCCAATGTGATCCA
  TGCCACAGTGACCTCTGTGACCCTGCACAGCACAGAGGGGAAAGCCCTGTACCAGGTGGCGTATGAGAAT
  GAGGTAGGCAACAGCTCTGACTTCTATGACATCGTGGTCATCGCCACCCCCCTGCACCTGGACAACAGCA
  GCAGCAACTTAACCTTTGCAGGCTTCCACCCGCCCATTGATGACGTGCAGGGCTCTTTCCAGCCCACCGT
  CGTCTCCTTGGTCCACGGCTACCTCAACTCGTCCTACTTCGGTTTCCCAGACCCTAAGCTTTTCCCCTTT
  GCCAACATCCTTACCACAGATTTCCCCAGCTTCTTCTGCACTCTGGACAACATCTGCCCTGTCAACATCT
  CTGCCAGCTTCCGGCGAAAGCAGCCCCAGGAGGCAGCTGTTTGGCGAGTCCAGTCCCCCAAGCCCCTCTT
  TCGGACCCAGCTAAAGACCCTGTTCCGTTCCTATTACTCAGTGCAGACAGCTGAGTGGCAGGCCCATCCC
  CTCTATGGCTCCCGCCCCACGCTCCCGAGGTTTGCACTCCATGACCAGCTCTTCTACCTCAATGCCCTGG
  AGTGGGCGGCCAGCTCCGTGGAGGTGATGGCCGTGGCTGCCAAGAATGTGGCCTTGCTGGCTTACAACCG
  CTGGTACCAGGACCTAGACAAGATTGATCAAAAAGATTTGATGCACAAGGTCAAGACTGAACTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC238112 representing NM_001301057
  Red=Cloning site Green=Tags(s)
  
  MPRRAKSVREGRAVVGAGIGGSAVAHFLQQHFGPRVQIDVYEKGTVGGRLATISVNKQHYESGAASFHSL
  SLHMQDFVKLLGLRHRREVVGRSAIFGGEHFMLEETDWYLLNLFRLWWHYGISFLRLQMWVEEVMEKFMS
  YGQSAAMPAFAGAMSLAGAQGSLWSVEGGNKLVCSGLLKLTKANVIHATVTSVTLHSTEGKALYQVAYEN
  EVGNSSDFYDIVVIATPLHLDNSSSNLTFAGFHPPIDDVQGSFQPTVVSLVHGYLNSSYFGFPDPKLFPF
  ANILTTDFPSFFCTLDNICPVNISASFRRKQPQEAAVWRVQSPKPLFRTQLKTLFRSYYSVQTAEWQAHP
  LYGSRPTLPRFALHDQLFYLNALEWAASSVEVMAVAAKNVALLAYNRWYQDLDKIDQKDLMHKVKTEL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001301057
• ORF Size: 1254 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001301057.2
• RefSeq Size: 2406 bp
• RefSeq ORF: 1257 bp
• Locus ID: 78991
• UniProt ID: Q8NBM8
• Cytogenetics: 5q32
• MW: 47.3 kDa
• Gene Summary: Probable oxidoreductase.[UniProtKB/Swiss-Prot Function]