MOGAT3 (NM_001287147) Human Tagged ORF Clone

CAT#: RC237148

MOGAT3 (myc-DDK-tagged) - Human monoacylglycerol O-acyltransferase 3 (MOGAT3), transcript variant 2

Product Images

Circular map for RC237148

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MOGAT3
• Synonyms: DC7; DGAT2L2; MGAT3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC237148 representing NM_001287147
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAGTTGCCACAACCCTGCAGCCCCCAACCACTTCCAAAACCTTGCAGAAGCAGCATCTAGAAGCAG
  TGGGCGCCTACCAATATGTGCTCACTTTCCTCTTCATGGGCCCTTTCTTCTCCCTTCTTGTCTTTGTCCT
  CCTCTTCACGTCACTCTGGCCCTTCTCTGTTTTTTACTTGGTGTGGCTCTATGTGGACTGGGACACACCC
  AACCAAGGTGGAAGGCGTTCGGAGTGGATAAGGAACCGGGCAATTTGGAGACAACTAAGGGATTATTATC
  CTGTCAAGCTGGTGAAAACAGCAGAGCTGCCCCCGGATCGGAACTACGTGCTGGGCGCCCACCCTCATGG
  GATCATGTGTACAGGCTTCCTCTGTAATTTCTCCACCGAGAGCAATGGCTTCTCCCAGCTCTTCCCGGGG
  CTCCGGCCCTGGTTAGCCGTGCTGGCTGGCCTCTTCTACCTCCCGGTCTATCGCGACTACATCATGTCCT
  TTGGACTCTGTCCGGTGAGCCGCCAGAGCCTGGACTTCATCCTGTCCCAGCCCCAGCTCGGGCAGGCCGT
  GGTCATCATGGTGGGGGGTGCGCACGAGGCCCTGTATTCAGTCCCCGGGGAGCACTGCCTTACGCTCCAG
  AAGCGCAAAGGCTTCGTGCGCCTGGCGCTGAGGCACGGTGGGCCGCCCCATCCCCGTCCCCCAGCGCCTC
  CACCCCACCGAGGAGGAAGTCAATCACTATCACGCCCTCTACATGACGGCCCTGGAGCAGCTCTTCGAGG
  AGCACAAGGAAAGCTGTGGGGTCCCCGCTTCCACCTGCCTCACCTTCATCTAGGCCTGGCCGCGGCCTTT
  CGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC237148 representing NM_001287147
  Red=Cloning site Green=Tags(s)
  
  MGVATTLQPPTTSKTLQKQHLEAVGAYQYVLTFLFMGPFFSLLVFVLLFTSLWPFSVFYLVWLYVDWDTP
  NQGGRRSEWIRNRAIWRQLRDYYPVKLVKTAELPPDRNYVLGAHPHGIMCTGFLCNFSTESNGFSQLFPG
  LRPWLAVLAGLFYLPVYRDYIMSFGLCPVSRQSLDFILSQPQLGQAVVIMVGGAHEALYSVPGEHCLTLQ
  KRKGFVRLALRHGGPPHPRPPAPPPHRGGSQSLSRPLHDGPGAALRGAQGKLWGPRFHLPHLHLGLAAAF
  R
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001287147
• ORF Size: 843 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001287147.2
• RefSeq Size: 1207 bp
• RefSeq ORF: 846 bp
• Locus ID: 346606
• UniProt ID: Q86VF5
• Cytogenetics: 7q22.1
• Protein Families: Transmembrane
• MW: 31.9 kDa
• Gene Summary: Acyl-CoA:monoacylglycerol acyltransferase (MOGAT; EC 2.3.1.22) catalyzes the synthesis of diacylglycerol from 2-monoacylglycerol and fatty acyl-CoA (Cheng et al., 2003 [PubMed 12618427]).[supplied by OMIM, Mar 2008]