BRCAA1 (ARID4B) (NM_001206794) Human Tagged ORF Clone
CAT#: RC235263
- TrueORF®
ARID4B (Myc-DDK tagged) - Homo sapiens AT rich interactive domain 4B (RBP1-like) (ARID4B), transcript variant 3
ORF Plasmid: tGFP
"NM_001206794" in other vectors (2)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | BRCAA1 |
Synonyms | BCAA; BRCAA1; RBBP1L1; RBP1L1; SAP180 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC235263 representing NM_001206794
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGGCCCTTGATGAGCCTCCCTATTTGACAGTGGGCACTGATGTGAGTGCTAAATACAGAGGAGCCT TTTGTGAAGCCAAGATCAAGACAGCAAAAAGACTTGTCAAAGTCAAGGTGACATTTAGACATGATTCTTC AACAGTGGAAGTTCAGGATGACCACATAAAGGGCCCACTAAAGGTAGGAGCTATTGTGGAAGTGAAGAAT CTTGATGGTGCATATCAGGAAGCTGTTATCAATAAACTAACAGATGCGAGTTGGTACACTGTAGTTTTTG ATGACGGAGATGAGAAGACACTGAGACGATCTTCACTGTGCCTGAAAGGAGAGAGGCATTTTGCTGAAAG TGAAACATTAGACCAGCTCCCACTCACCAACCCTGAGCATTTTGGCACTCCAGTCATAGGAAAGAAAACA AATAGAGGAAGAAGATCTAATCATATACCAGAGGAAGAGTCTTCATCATCCTCCAGTGATGAAGATGAGG ATGATAGGAAACAGATTGATGAGCTACTAGGCAAAGTTGTATGTGTAGATTACATTAGTTTGGATAAAAA GAAAGCACTGTGGTTTCCTGCATTGGTGGTTTGTCCTGATTGTAGTGATGAGATTGCTGTAAAAAAGGAC AATATTCTTGTTCGATCTTTCAAAGATGGAAAATTTACTTCAGTTCCAAGAAAAGATGTCCATGAAATTA CTAGTGACACTGCACCAAAGCCTGATGCTGTTTTAAAGCAAGCCTTTGAACAGGCACTTGAATTTCACAA AAGTAGAACTATTCCTGCTAACTGGAAGACTGAATTGAAAGAAGATAGCTCTAGCAGTGAAGCAGAGGAA GAAGAGGAGGAGGAAGATGATGAAAAAGAAAAGGAGGATAATAGCAGTGAAGAAGAAGAAGAAATAGAAC CATTTCCAGAAGAAAGGGAGAACTTTCTTCAGCAATTGTACAAATTTATGGAAGATAGAGGTACACCTAT TAACAAACGACCTGTACTTGGATATCGAAATTTGAATCTCTTTAAGTTATTCAGACTTGTACACAAACTT GGAGGATTTGATAATATTGAAAGTGGAGCTGTTTGGAAACAAGTCTACCAAGATCTTGGAATCCCTGTCT TAAATTCAGCTGCAGGATACAATGTTAAATGTGCTTATAAAAAATACTTATATGGTTTTGAGGAGTACTG TAGATCAGCCAACATTGAATTTCAGATGGCATTGCCAGAGAAAGTTGTTAACAAGCAATGTAAGGAGTGT GAAAATGTAAAAGAAATAAAAGTTAAGGAGGAAAATGAAACAGAGATCAAAGAAATAAAGATGGAGGAGG AGAGGAATATAATACCAAGAGAAGAAAAGCCTATTGAGGATGAAATTGAAAGAAAAGAAAATATTAAGCC CTCTCTGGGAAGTAAAAAGAATTTATTAGAATCTATACCTACACATTCTGATCAGGAAAAAGAAGTTAAC ATTAAAAAACCAGAAGACAATGAAAATCTGGATGACAAAGATGATGACACAACTAGGGTAGATGAATCCC TCAACATAAAGGTAGAAGCTGAGGAAGAAAAAGCAAAATCTGGAGATGAAACGAATAAAGAAGAAGATGA AGATGATGAAGAAGCAGAAGAGGAGGAGGAGGAGGAAGAAGAAGAAGAGGATGAAGATGATGATGACAAC AATGAGGAAGAGGAGTTTGAGTGCTATCCACCAGGCATGAAAGTCCAAGTGCGGTATGGACGAGGGAAAA ATCAAAAAATGTATGAAGCTAGTATTAAAGATTCTGATGTCGAAGGTGGAGAGGTCCTTTACTTGGTGCA TTACTGCGGATGGAATGTGAGATACGATGAATGGATTAAAGCAGATAAAATAGTAAGACCTGCTGATAAA AATGTGCCAAAGATAAAACATCGGAAGAAAATAAAGAATAAATTAGACAAAGAAAAAGACAAAGATGAAA AATACTCTCCAAAAAACTGTAAACTTCGGCGCTTGTCCAAACCACCATTTCAGACAAATCCATCTCCTGA AATGGTATCCAAACTGGATCTCACTGATGCCAAAAACTCTGATACTGCTCATATTAAGTCCATAGAAATT ACTTCGATCCTTAATGGACTTCAAGCTTCTGAAAGTTCTGCTGAAGACAGTGAGCAGGAAGATGAGAGAG GTGCTCAAGACATGGATAATAATGGCAAAGAGGAATCTAAGATTGATCATTTGACCAACAACAGAAATGA TCTTATTTCAAAGGAGGAACAGAACAGTTCATCTTTGCTAGAAGAAAACAAAGTTCATGCAGATTTGGTA ATATCCAAACCAGTGTCAAAATCTCCAGAAAGATTAAGGAAAGATATAGAAGTATTATCCGAAGATACTG ATTATGAAGAAGATGAAGTCACAAAAAAGAGAAAGGATGTCAAGAAGGACACAACAGATAAATCTTCAAA ACCACAAATAAAACGTGGTAAAAGAAGGTATTGCAATACAGAAGAGTGTCTAAAAACTGGATCACCTGGC AAAAAGGAAGAGAAGGCCAAGAACAAAGAATCACTTTGCATGGAAAACAGTAGCAACAGCTCTTCAGATG AAGATGAAGAAGAAACAAAAGCAAAGATGACACCAACTAAGAAATACAATGGTTTGGAGGAAAAAAGAAA ATCTCTACGGACAACTGGTTTCTATTCAGGATTTTCAGAAGTGGCAGAAAAAAGGATTAAACTTTTAAAT AACTCTGATGAAAGACTTCAAAACAGCAGGGCCAAAGATCGAAAAGATGTCTGGTCAAGTATTCAGGGAC AGTGGCCTAAAAAAACGCTGAAAGAGCTTTTTTCAGACTCTGATACTGAGGCTGCAGCTTCCCCACCGCA TCCTGCCCCAGAGGAGGGGGTGGCAGAGGAGTCACTGCAGACTGTGGCTGAAGAGGAGAGTTGTTCACCC AGTGTAGAACTAGAAAAACCACCTCCAGTCAATGTCGATAGTAAACCCATTGAAGAAAAAACAGTAGAGG TCAATGACAGAAAAGCAGAATTTCCAAGTAGTGGCAGTAATTCAGTGCTAAATACCCCTCCTACTACACC TGAATCGCCTTCATCAGTCACTGTAACAGAAGGCAGCCGGCAGCAGTCTTCTGTAACAGTATCAGAACCA CTGGCTCCAAACCAAGAAGAGGTTCGAAGTATCAAGAGTGAAACTGATAGCACAATTGAGGTGGATAGTG TTGCTGGGGAGCTCCAAGACCTCCAGTCTGAAGGGAATAGCTCGCCAGCAGGTTTTGATGCCAGTGTGAG CTCAAGCAGTAGTAATCAGCCAGAACCAGAACATCCTGAAAAAGCCTGTACAGGTCAGAAAAGAGTGAAA GATGCTCAGGGAGGAGGAAGTTCATCAAAAAAGCAGAAAAGAAGCCATAAAGCAACAGTGGTAAACAACA AAAAGAAGGGAAAAGGCACAAATAGTAGTGATAGTGAAGAACTTTCAGCTGGTGAAAGTATAACTAAGAG TCAGCCAGTCAAATCAGTTTCCACTGGAATGAAGTCTCATAGTACCAAATCTCCCGCAAGGACGCAGTCT CCAGGAAAATGTGGAAAGAATGGTGATAAGGATCCTGATCTCAAGGAACCCAGTAATCGATTACCCAAAG TTTACAAATGGAGTTTTCAGATGTCGGACCTGGAAAATATGACAAGTGCCGAACGCATCACAATTCTTCA AGAAAAACTTCAAGAAATCAGAAAACATTATCTGTCATTAAAATCTGAAGTAGCTTCCATTGATCGGAGG AGAAAGCGTTTAAAGAAGAAAGAGAGAGAAAGTGCTGCTACATCCTCATCCTCCTCTTCACCTTCATCCA GTTCCATAACAGCTGCTGTTATGTTAACTTTAGCTGAACCGTCAATGTCCAGCGCATCACAAAATGGAAT GTCAGTTGAGTGCAGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC235263 representing NM_001206794
Red=Cloning site Green=Tags(s) MKALDEPPYLTVGTDVSAKYRGAFCEAKIKTAKRLVKVKVTFRHDSSTVEVQDDHIKGPLKVGAIVEVKN LDGAYQEAVINKLTDASWYTVVFDDGDEKTLRRSSLCLKGERHFAESETLDQLPLTNPEHFGTPVIGKKT NRGRRSNHIPEEESSSSSSDEDEDDRKQIDELLGKVVCVDYISLDKKKALWFPALVVCPDCSDEIAVKKD NILVRSFKDGKFTSVPRKDVHEITSDTAPKPDAVLKQAFEQALEFHKSRTIPANWKTELKEDSSSSEAEE EEEEEDDEKEKEDNSSEEEEEIEPFPEERENFLQQLYKFMEDRGTPINKRPVLGYRNLNLFKLFRLVHKL GGFDNIESGAVWKQVYQDLGIPVLNSAAGYNVKCAYKKYLYGFEEYCRSANIEFQMALPEKVVNKQCKEC ENVKEIKVKEENETEIKEIKMEEERNIIPREEKPIEDEIERKENIKPSLGSKKNLLESIPTHSDQEKEVN IKKPEDNENLDDKDDDTTRVDESLNIKVEAEEEKAKSGDETNKEEDEDDEEAEEEEEEEEEEEDEDDDDN NEEEEFECYPPGMKVQVRYGRGKNQKMYEASIKDSDVEGGEVLYLVHYCGWNVRYDEWIKADKIVRPADK NVPKIKHRKKIKNKLDKEKDKDEKYSPKNCKLRRLSKPPFQTNPSPEMVSKLDLTDAKNSDTAHIKSIEI TSILNGLQASESSAEDSEQEDERGAQDMDNNGKEESKIDHLTNNRNDLISKEEQNSSSLLEENKVHADLV ISKPVSKSPERLRKDIEVLSEDTDYEEDEVTKKRKDVKKDTTDKSSKPQIKRGKRRYCNTEECLKTGSPG KKEEKAKNKESLCMENSSNSSSDEDEEETKAKMTPTKKYNGLEEKRKSLRTTGFYSGFSEVAEKRIKLLN NSDERLQNSRAKDRKDVWSSIQGQWPKKTLKELFSDSDTEAAASPPHPAPEEGVAEESLQTVAEEESCSP SVELEKPPPVNVDSKPIEEKTVEVNDRKAEFPSSGSNSVLNTPPTTPESPSSVTVTEGSRQQSSVTVSEP LAPNQEEVRSIKSETDSTIEVDSVAGELQDLQSEGNSSPAGFDASVSSSSSNQPEPEHPEKACTGQKRVK DAQGGGSSSKKQKRSHKATVVNNKKKGKGTNSSDSEELSAGESITKSQPVKSVSTGMKSHSTKSPARTQS PGKCGKNGDKDPDLKEPSNRLPKVYKWSFQMSDLENMTSAERITILQEKLQEIRKHYLSLKSEVASIDRR RKRLKKKERESAATSSSSSSPSSSSITAAVMLTLAEPSMSSASQNGMSVECR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001206794 |
ORF Size | 3936 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001206794.1, NP_001193723.1 |
RefSeq Size | 5954 bp |
RefSeq ORF | 3939 bp |
Locus ID | 51742 |
UniProt ID | Q4LE39 |
Cytogenetics | 1q42.3 |
MW | 148.3 kDa |
Gene Summary | This gene encodes a protein with sequence similarity to retinoblastoma-binding protein-1. The encoded protein is a subunit of the histone deacetylase-dependant SIN3A transcriptional corepressor complex, which functions in diverse cellular processes including proliferation, differentiation, apoptosis, oncogenesis, and cell fate determination. The gene product is recognized by IgG antibody isolated from a breast cancer patient and appears to be a molecular marker associated with a broad range of human malignancies. Alternate transcriptional splice variants encoding different isoforms have been characterized. [provided by RefSeq, Jul 2008] |
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