SPHK2 (NM_001243876) Human Tagged ORF Clone

CAT#: RC234130

SPHK2 (Myc-DDK tagged) - Homo sapiens sphingosine kinase 2 (SPHK2), transcript variant 5

Product Images

Circular map for RC234130

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SPHK2
• Synonyms: SK-2; SK 2; SPK-2; SPK 2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC234130 representing NM_001243876
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATCTCTGAAGCTGGGCTGTCCTTCAACCTCATCCAGACAGAACGACAGAACCACGCCCGGGAGCTGG
  TCCAGGGGCTGAGCCTGAGTGAGTGGGATGGCATCGTCACGGTCTCGGGAGACGGGCTGCTCCATGAGGT
  GCTGAACGGGCTCCTAGATCGCCCTGACTGGGAGGAAGCTGTGAAGATGCCTGTGGGCATCCTCCCCTGC
  GGCTCGGGCAACGCGCTGGCCGGAGCAGTGAACCAGCACGGGGGATTTGAGCCAGCCCTGGGCCTCGACC
  TGTTGCTCAACTGCTCACTGTTGCTGTGCCGGGGTGGTGGCCACCCACTGGACCTGCTCTCCGTGACGCT
  GGCCTCGGGCTCCCGCTGTTTCTCCTTCCTGTCTGTGGCCTGGGGCTTCGTGTCAGATGTGGATATCCAG
  AGCGAGCGCTTCAGGGCCTTGGGCAGTGCCCGCTTCACACTGGGCACGGTGCTGGGCCTCGCCACACTGC
  ACACCTACCGCGGACGCCTCTCCTACCTCCCCGCCACTGTGGAACCTGCCTCGCCCACCCCTGCCCATAG
  CCTGCCTCGTGCCAAGTCGGAGCTGACCCTAACCCCAGACCCAGCCCCGCCCATGGCCCACTCACCCCTG
  CATCGTTCTGTGTCTGACCTGCCTCTTCCCCTGCCCCAGCCTGCCCTGGCCTCTCCTGGCTCGCCAGAAC
  CCCTGCCCATCCTGTCCCTCAACGGTGGGGGCCCAGAGCTGGCTGGGGACTGGGGTGGGGCTGGGGATGC
  TCCGCTGTCCCCGGACCCACTGCTGTCTTCACCTCCTGGCTCTCCCAAGGCAGCTCTACACTCACCCGTC
  TCCGAAGGGGCCCCCGTAATTCCCCCATCCTCTGGGCTCCCACTTCCCACCCCTGATGCCCGGGTAGGGG
  CCTCCACCTGCGGCCCGCCCGACCACCTGCTGCCTCCGCTGGGCACCCCGCTGCCCCCAGACTGGGTGAC
  GCTGGAGGGGGACTTTGTGCTCATGTTGGCCATCTCGCCCAGCCACCTAGGCGCTGACCTGGTGGCAGCT
  CCGCATGCGCGCTTCGACGACGGCCTGGTGCACCTGTGCTGGGTGCGTAGCGGCATCTCGCGGGCTGCGC
  TGCTGCGCCTTTTCTTGGCCATGGAGCGTGGTAGCCACTTCAGCCTGGGCTGTCCGCAGCTGGGCTACGC
  CGCGGCCCGTGCCTTCCGCCTAGAGCCGCTCACACCACGCGGCGTGCTCACAGTGGACGGGGAGCAGGTG
  GAGTATGGGCCGCTACAGGCACAGATGCACCCTGGCATCGGTACACTGCTCACTGGGCCTCCTGGCTGCC
  CGGGGCGGGAGCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC234130 representing NM_001243876
  Red=Cloning site Green=Tags(s)
  
  MISEAGLSFNLIQTERQNHARELVQGLSLSEWDGIVTVSGDGLLHEVLNGLLDRPDWEEAVKMPVGILPC
  GSGNALAGAVNQHGGFEPALGLDLLLNCSLLLCRGGGHPLDLLSVTLASGSRCFSFLSVAWGFVSDVDIQ
  SERFRALGSARFTLGTVLGLATLHTYRGRLSYLPATVEPASPTPAHSLPRAKSELTLTPDPAPPMAHSPL
  HRSVSDLPLPLPQPALASPGSPEPLPILSLNGGGPELAGDWGGAGDAPLSPDPLLSSPPGSPKAALHSPV
  SEGAPVIPPSSGLPLPTPDARVGASTCGPPDHLLPPLGTPLPPDWVTLEGDFVLMLAISPSHLGADLVAA
  PHARFDDGLVHLCWVRSGISRAALLRLFLAMERGSHFSLGCPQLGYAAARAFRLEPLTPRGVLTVDGEQV
  EYGPLQAQMHPGIGTLLTGPPGCPGREP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001243876
• ORF Size: 1344 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001243876.1, NP_001230805.1
• RefSeq Size: 2492 bp
• RefSeq ORF: 1347 bp
• Locus ID: 56848
• Cytogenetics: 19q13.33
• Protein Families: Druggable Genome
• Protein Pathways: Calcium signaling pathway, Fc gamma R-mediated phagocytosis, Metabolic pathways, Sphingolipid metabolism, VEGF signaling pathway
• MW: 47 kDa
• Gene Summary: This gene encodes one of two sphingosine kinase isozymes that catalyze the phosphorylation of sphingosine into sphingosine 1-phosphate. Sphingosine 1-phosphate mediates many cellular processes including migration, proliferation and apoptosis, and also plays a role in several types of cancer by promoting angiogenesis and tumorigenesis. The encoded protein may play a role in breast cancer proliferation and chemoresistance. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2011]