IKZF5 (NM_001271840) Human Tagged ORF Clone

CAT#: RC234044

IKZF5 (Myc-DDK tagged) - Homo sapiens IKAROS family zinc finger 5 (Pegasus) (IKZF5), transcript variant 1

Product Images

Circular map for RC234044

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: IKZF5
• Synonyms: PEGASUS; THC7; ZNFN1A5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC234044 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTGAAAAAAAACCAGAGCCTTTGGACTTCGTGAAAGATTTTCAGGAATACCTGACTCAGCAGACCC
  ATCACGTGAACATGATTTCTGGATCAGTTAGTGGGGACAAAGAAGCAGAGGCTCTTCAGGGAGCTGGAAC
  AGATGGTGATCAAAATGGACTTGATCACCCATCTGTTGAAGTTTCCTTGGATGAAAACTCAGGAATGTTA
  GTAGACGGGTTTGAAAGGACCTTTGATGGGAAGCTTAAGTGTCGGTACTGCAACTATGCCAGCAAAGGAA
  CAGCCCGGCTTATTGAACACATCAGAATCCACACAGGTGAAAAACCTCATCGATGTCATCTTTGTCCATT
  TGCATCTGCTTATGAGCGTCATCTGGAAGCCCATATGCGTTCTCATACTGGAGAAAAACCATACAAATGT
  GAATTATGTTCCTTCCGCTGCAGTGATCGAAGTAACTTGTCCCATCATCGAAGGCGCAAGCATAAAATGG
  TACCAATTAAAGGTACTAGGTCTTCCTTAAGCAGCAAGAAAATGTGGGGGGTTTTACAGAAGAAAACAAG
  CAATCTGGGCTATAGCAGAAGAGCACTAATCAACTTAAGTCCACCTTCCATGGTGGTTCAGAAACCAGAC
  TACCTTAACGATTTTACCCACGAAATCCCAAATATCCAGACTGACTCCTATGAAAGTATGGCAAAAACCA
  CACCAACTGGTGGCCTTCCAAGGGACCCCCAAGAACTCATGGTTGATAACCCTTTGAATCAGCTCTCGAC
  TCTAGCAGGGCAGTTGTCCAGTCTGCCACCCGAAAACCAAAACCCTGCATCCCCTGATGTAGTTCCCTGC
  CCTGATGAAAAGCCTTTCATGATTCAGCAGCCCTCTACCCAAGCAGTAGTTTCTGCCGTATCAGCAAGTA
  TTCCTCAGAGCTCCTCTCCCACAAGCCCAGAACCTCGGCCATCCCATAGTCAAAGGAACTATAGTCCAGT
  GGCAGGTCCAAGCAGTGAGCCAAGTGCCCACACGAGCACTCCCAGCATAGGAAACAGCCAGCCAAGCACC
  CCAGCCCCAGCCCTGCCGGTCCAGGACCCTCAGCTTCTGCACCACTGCCAGCACTGTGATATGTACTTTG
  CAGACAACATCCTTTACACTATTCATATGGGATGTCATGGGTATGAAAATCCTTTTCAGTGTAATATATG
  TGGATGCAAATGTAAAAACAAGTATGATTTTGCCTGTCATTTTGCAAGAGGGCAACATAACCAACAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC234044 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGEKKPEPLDFVKDFQEYLTQQTHHVNMISGSVSGDKEAEALQGAGTDGDQNGLDHPSVEVSLDENSGML
  VDGFERTFDGKLKCRYCNYASKGTARLIEHIRIHTGEKPHRCHLCPFASAYERHLEAHMRSHTGEKPYKC
  ELCSFRCSDRSNLSHHRRRKHKMVPIKGTRSSLSSKKMWGVLQKKTSNLGYSRRALINLSPPSMVVQKPD
  YLNDFTHEIPNIQTDSYESMAKTTPTGGLPRDPQELMVDNPLNQLSTLAGQLSSLPPENQNPASPDVVPC
  PDEKPFMIQQPSTQAVVSAVSASIPQSSSPTSPEPRPSHSQRNYSPVAGPSSEPSAHTSTPSIGNSQPST
  PAPALPVQDPQLLHHCQHCDMYFADNILYTIHMGCHGYENPFQCNICGCKCKNKYDFACHFARGQHNQH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001271840
• ORF Size: 1257 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001271840.1, NP_001258769.1
• RefSeq Size: 4553 bp
• RefSeq ORF: 1260 bp
• Locus ID: 64376
• UniProt ID: Q9H5V7
• Cytogenetics: 10q26.13
• MW: 46.5 kDa
• Gene Summary: Members of the Ikaros (ZNFN1A1; MIM 603023) family of transcription factors, which includes Pegasus, are expressed in lymphocytes and are implicated in the control of lymphoid development.[supplied by OMIM, Jul 2002]