CENPN (NM_001270474) Human Tagged ORF Clone

CAT#: RC233703

CENPN (Myc-DDK tagged) - Homo sapiens centromere protein N (CENPN), transcript variant 5

Product Images

Circular map for RC233703

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CENPN
• Synonyms: BM039; C16orf60; CENP-N; ICEN32
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC233703 representing NM_001270474
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGAGACTGTTGCTGAGTTCATCAAGAGGACCATCTTGAAAATCCCCATGAATGAACTGACAACAA
  TCCTGAAGGCCTGGGATTTTTTGTCTGAAAATCAACTGCAGACTGTAAATTTCCGACAGAGAAAGGAATC
  TGTAGTTCAGCACTTGATCCATCTGTGTGAGGAAAAGCGTGCAAGTATCAGTGATGCTGCCCTGTTAGAC
  ATCATTTATATGCAATTTCATCAGCACCAGAAAGTTTGGGAAGTTTTTCAGATGAGTAAAGGACCAGGTG
  AAGATGTTGACCTTTTTGATATGAAACAATTTAAAAATTCGTTCAAGAAAATTCTTCAGAGAGCATTAAA
  AAATGTGACAGTCAGCTTCAGAGAAACTGAGGAGAATGCAGTCTGGATTCGAATTGCCTGGGGAACACAG
  TACACAAAGCCAAACCAGTACAAACCTACCTACGTGGTGTACTACTCCCAGACTCCGTACGCCTTCACGT
  CCTCCTCCATGCTGAGGCGCAATACACCGCTTCTGGGTCAGACCTTTGAAACTCACAACTCTACGACACC
  TCTACAGGAAAGAAGCCTTGGACTAGATATAAATATGGATTCAAGGATCATTCATGAAAACATAGTAGAA
  AAAGAGAGAGTCCAACGAATAACTCAAGAAACATTTGGAGATTATCCTCAACCACAACTAGAATTTGCAC
  AATATAAGCTTGAAACGAAATTCAAAAGTGGTTTAAATGGGAGCATCTTGGCTGAGAGGGAAGAACCCCT
  CCGATGCCTAATAAAGTTCTCTAGCCCACATCTTCTGGAAGCATTGAAATCCTTAGCACCAGCGGGTATT
  GCAGATGCTCCACTTTCTCCACTGCTCACTTGCATACCCAACAAGAGAATGAATTATTTTAAAATTAGAG
  ATAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC233703 representing NM_001270474
  Red=Cloning site Green=Tags(s)
  
  MDETVAEFIKRTILKIPMNELTTILKAWDFLSENQLQTVNFRQRKESVVQHLIHLCEEKRASISDAALLD
  IIYMQFHQHQKVWEVFQMSKGPGEDVDLFDMKQFKNSFKKILQRALKNVTVSFRETEENAVWIRIAWGTQ
  YTKPNQYKPTYVVYYSQTPYAFTSSSMLRRNTPLLGQTFETHNSTTPLQERSLGLDINMDSRIIHENIVE
  KERVQRITQETFGDYPQPQLEFAQYKLETKFKSGLNGSILAEREEPLRCLIKFSSPHLLEALKSLAPAGI
  ADAPLSPLLTCIPNKRMNYFKIRDK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001270474
• ORF Size: 915 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001270474.1, NP_001257403.1
• RefSeq Size: 4561 bp
• RefSeq ORF: 918 bp
• Locus ID: 55839
• UniProt ID: Q96H22
• Cytogenetics: 16q23.2
• Protein Families: Druggable Genome
• MW: 36 kDa
• Gene Summary: The protein encoded by this gene forms part of the nucleosome-associated complex and is important for kinetochore assembly. It is bound to kinetochores during S phase and G2 and recruits other proteins to the centromere. Pseudogenes of this gene are located on chromosome 2. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Jul 2012]