Calcipressin 3 (RCAN3) (NM_001251979) Human Tagged ORF Clone

CAT#: RC233528

RCAN3 (Myc-DDK tagged) - Homo sapiens RCAN family member 3 (RCAN3), transcript variant 4

Product Images

Circular map for RC233528

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Calcipressin 3
• Synonyms: DSCR1L2; hRCN3; MCIP3; RCN3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC233528 representing NM_001251979.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGCTGAGGGACACTATGAAATCTTGGAATGATAGCCAGTCAGATCTGTGTAGCACTGACCAAGAAGAG
  GAAGAAGAGATGATTTTTGGTGAAAATGAAGATGATTTGGATGAGATGATGGATTTAAGTGATCTGCCT
  ACCTCACTTTTTGCTTGCAGCGTCCATGAAGCAGTGTTTGAGGCACGAGAGCAGAAGGAAAGATTTGAA
  GCACTCTTCACCATCTATGATGACCAGGTTACTTTTCAGCTGTTTAAAAGCTTTAGAAGAGTCAGAATA
  AATTTCAGCAAACCTGAAGCGGCAGCAAGAGCGCGAATAGAACTCCACGAAACAGACTTCAATGGGCAG
  AAGCTAAAGCTATATTTTGCACAGGTGCAGATGTCCGGCGAAGTGCGGGACAAGTCCTATCTCCTGCCA
  CCCCAGCCTGTCAAGCAGTTCCTCATCTCCCCTCCAGCCTCTCCCCCAGTGGGGTGGAAGCAGAGCGAA
  GATGCGATGCCTGTTATAAATTATGATTTACTCTGTGCTGTTTCCAAATTGGGACCAGGAGAGAAATAT
  GAACTTCACGCGGGAACAGAGTCGACACCCAGCGTGGTGGTTCATGTCTGTGAAAGTGAAACTGAAGAG
  GAAGAAGAGACAAAAAACCCCAAACAGAAAATTGCCCAGACAAGGCGCCCCGACCCTCCGACCGCAGCG
  TTGAATGAGCCCCAGACCTTTGATTGCGCGCTG
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
  Protein Sequence

  >Peptide sequence encoded by RC233528
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MLRDTMKSWNDSQSDLCSTDQEEEEEMIFGENEDDLDEMMDLSDLPTSLFACSVHEAVFEAREQKERFE
  ALFTIYDDQVTFQLFKSFRRVRINFSKPEAAARARIELHETDFNGQKLKLYFAQVQMSGEVRDKSYLLP
  PQPVKQFLISPPASPPVGWKQSEDAMPVINYDLLCAVSKLGPGEKYELHAGTESTPSVVVHVCESETEE
  EEETKNPKQKIAQTRRPDPPTAALNEPQTFDCAL
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001251979
• ORF Size: 723 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq Size: 2646 bp
• RefSeq ORF: 726 bp
• Locus ID: 11123
• UniProt ID: Q9UKA8
• Cytogenetics: 1p36.11
• MW: 27.5 kDa
• Gene Summary: Inhibits calcineurin-dependent transcriptional responses by binding to the catalytic domain of calcineurin A. Could play a role during central nervous system development (By similarity).[UniProtKB/Swiss-Prot Function]