Intersectin 2 (ITSN2) (NM_147152) Human Tagged ORF Clone
CAT#: RC229421
- TrueORF®
ITSN2 (Myc-DDK-tagged)-Human intersectin 2 (ITSN2), transcript variant 2
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_147152" in other vectors (5)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Intersectin 2 |
Synonyms | PRO2015; SH3D1B; SH3P18; SWA; SWAP |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC229421 representing NM_147152
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGGCTCAGTTTCCCACAGCTATGAATGGAGGGCCAAACATGTGGGCTATTACCTCTGAAGAACGTA CTAAGCATGACAGGCAGTTTGATAACCTCAAACCTTCAGGAGGTTACATAACAGGTGATCAAGCACGTAA TTTTTTCCTACAATCAGGTCTGCCGGCCCCTGTTTTAGCTGAAATATGGGCTTTATCAGACCTAAACAAG GATGGGAAGATGGATCAGCAAGAGTTCTCCATAGCTATGAAACTCATCAAACTGAAGCTTCAAGGCCAAC AGTTGCCTGTGGTTCTCCCTCCTATTATGAAGCAACCCCCTATGTTTTCTCCATTAATTTCTGCTCGTTT TGGAATGGGAAGCATGCCCAATCTGTCCATTCCTCAGCCATTGCCTCCAGCTGCACCTATAACATCATTG TCTTCTGCGACTTCAGGGACCAACCTTCCTCCCTTAATGATGCCCACTCCCCTAGTGCCTTCTGTTAGCA CATCATCATTACCAAATGGAACCGCCAGTCTCATTCAGCCTTTACCCATTCCTTATTCTTCTTCAACATT GCCTCATGGGTCATCTTATAGTCTGATGATGGGAGGATTTGGAGGTGCTAGTATACAGAAAGCGCAGTCT CTGATTGATTTAGGATCTAGTAGCTCAACTTCCTCGACTGCTTCACTCTCAGGGAACTCACCCAAGACTG GGACCTCAGAGTGGGCAGTTCCTCAGCCTACAAGATTAAAATATCGGCAAAAATTTAATACTCTTGACAA AAGTATGAGTGGATATCTCTCAGGTTTTCAAGCTAGAAATGCCCTTCTTCAGTCAAATCTTTCTCAAACT CAGCTGGCTACTATTTGGACTCTGGCTGACGTTGATGGTGATGGACAGCTAAAAGCAGAAGAGTTTATTC TTGCAATGCACCTTACTGACATGGCCAAAGCTGGACAGCCATTACCACTGACTTTACCTCCTGAGCTTGT TCCTCCATCTTTCAGAGGAGGAAAGCAAATTGATTCCATTAATGGAACTCTGCCTTCATATCAGAAAATG CAAGAAGAGGAGCCTCAGAAGAAATTACCAGTTACTTTTGAGGACAAACGGAAAGCCAACTATGAGCGAG GGAACATGGAGCTGGAAAAGCGACGCCAAGCCTTGATGGAGCAGCAACAAAGGGAGGCAGAACGTAAAGC CCAGAAAGAAAAGGAAGAGTGGGAACGAAAACAGAGAGAATTACAAGAACAAGAATGGAAGAAACAACTT GAATTAGAAAAACGCTTAGAGAAGCAACGGGAATTGGAGAGACAACGAGAGGAAGAAAGGAGAAAAGACA TAGAAAGACGAGAGGCAGCAAAACAGGAACTTGAACGACAACGTCGCTTAGAATGGGAGAGAATTCGGCG ACAGGAGCTTCTCAATCAAAAGAATAGAGAACAAGAAGAAATTGTCAGGTTAAACTCTAAAAAGAAGAAT CTTCATCTTGAGTTGGAAGCACTGAATGGCAAACATCAGCAGATCTCAGGCAGACTTCAGGATGTCCGAC TCAAAAAGCAAACTCAAAAGACTGAGCTGGAAGTTCTGGATAAGCAGTGTGACTTGGAAATTATGGAAAT CAAGCAACTTCAACAGGAACTTCAGGAATATCAGAATAAGCTTATCTATCTGGTACCTGAGAAGCAATTA TTAAATGAAAGAATTAAAAACATGCAGTTCAGTAACACACCTGATTCAGGGGTCAGTTTACTTCATAAAA AATCATTAGAAAAGGAAGAATTATGCCAAAGACTTAAAGAACAGTTAGATGCTCTTGAAAAAGAAACTGC ATCTAAGCTGTCAGAAATGGATTCTTTTAACAATCAACTAAAGTGTGGGAATATGGATGACTCTGTTCTT CAGTGCCTTTTGTCTCTGCTAAGCTGTCTCAACAACCTCTTCCTCTTACTTAAGGAACTGAGAGAAACCT ACAACACACAGCAGTTAGCCCTTGAACAGCTTTATAAGATCAAACGTGACAAGTTGAAGGAAATTGAAAG GAAAAGATTAGAACTAATGCAGAAAAAGAAACTAGAAGATGAGGCTGCAAGGAAAGCAAAGCAAGGAAAA GAAAACTTATGGAAAGAAAATCTTAGAAAGGAGGAAGAAGAAAAACAAAAGCGACTCCAGGAAGAAAAAA CACAAGAAAAAATTCAAGAAGAGGAACGGAAAGCTGAGGAGAAACAACGTAAGGATAAGGATACTTTGAA AGCTGAGGAGAAAAAACGTGAGACAGCTAGTGTTTTGGTGAATTATAGAGCATTATACCCCTTTGAAGCA AGGAACCATGATGAGATGAGTTTTAATTCTGGAGATATAATTCAGGTTGATGAAAAAACCGTAGGAGAAC CTGGTTGGCTTTATGGTAGTTTTCAAGGAAATTTTGGCTGGTTTCCATGCAATTATGTAGAAAAAATGCC ATCAAGTGAAAATGAAAAAGCTGTATCTCCAAAGAAGGCCTTACTTCCTCCTACAGTTTCTTTATCTGCT ACCTCAACTTCCTCTGAACCACTTTCTTCAAATCAACCAGCATCAGTGACTGATTATCAAAATGTATCTT TTTCAAACCTAACTGTAAATACATCATGGCAGAAAAAATCAGCCTTCACTCGAACTGTGTCCCCTGGATC TGTATCACCTATTCATGGACAGGGACAAGTGGTAGAAAACTTAAAAGCACAGGCCCTTTGTTCCTGGACT GCAAAGAAAGATAACCACTTGAACTTCTCAAAACATGACATTATTACTGTCTTGGAGCAGCAAGAAAATT GGTGGTTTGGGGAGGTGCATGGAGGAAGAGGATGGTTTCCCAAATCTTATGTCAAGATCATTCCTGGGAG TGAAGTAAAACGGGAAGAACCAGAAGCTTTGTATGCAGCTGTAAATAAGAAACCTACCTCGGCAGCCTAT TCAGTTGGAGAAGAATATATTGCACTTTATCCATATTCAAGTGTGGAACCTGGAGATTTGACTTTCACAG AAGGTGAAGAAATATTGGTGACCCAGAAAGATGGAGAGTGGTGGACAGGAAGTATTGGAGATAGAAGTGG AATTTTTCCATCAAACTATGTCAAACCAAAGGATCAAGAGAGTTTTGGGAGTGCTAGCAAGTCTGGAGCA TCAAATAAAAAACCTGAGATTGCTCAGGTAACTTCAGCATATGTTGCTTCTGGTTCTGAACAACTTAGCC TTGCACCAGGACAGTTAATATTAATTCTAAAGAAAAATACAAGTGGGTGGTGGCAAGGAGAGTTACAGGC CAGAGGAAAAAAGCGACAGAAAGGATGGTTTCCTGCCAGTCATGTTAAACTTTTGGGTCCAAGTAGTGAA AGAGCCACACCTGCCTTTCATCCTGTATGTCAGGTGATTGCTATGTATGACTATGCAGCAAATAATGAAG ATGAGCTCAGTTTCTCCAAGGGACAACTCATTAATGTTATGAACAAAGATGATCCTGATTGGTGGCAAGG AGAGATCAACGGGGTGACTGGTCTCTTTCCTTCAAACTACGTTAAGATGACGACAGACTCAGATCCAAGT CAACAGTGGTGTGCTGATCTGCAAACCCTGGACACAATGCAGCCAATTGAGAGGAAAAGACAGGGCTATA TTCATGAGCTGATTCAGACCGAAGAGCGGTACATGGCTGACCTTCAGCTCGTCGTCGAGGTGAGGAGGCT GCTGCTGGCTAGCTCTCGGGGTATCTGCTGTCTCTCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC229421 representing NM_147152
Red=Cloning site Green=Tags(s) MMAQFPTAMNGGPNMWAITSEERTKHDRQFDNLKPSGGYITGDQARNFFLQSGLPAPVLAEIWALSDLNK DGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPIMKQPPMFSPLISARFGMGSMPNLSIPQPLPPAAPITSL SSATSGTNLPPLMMPTPLVPSVSTSSLPNGTASLIQPLPIPYSSSTLPHGSSYSLMMGGFGGASIQKAQS LIDLGSSSSTSSTASLSGNSPKTGTSEWAVPQPTRLKYRQKFNTLDKSMSGYLSGFQARNALLQSNLSQT QLATIWTLADVDGDGQLKAEEFILAMHLTDMAKAGQPLPLTLPPELVPPSFRGGKQIDSINGTLPSYQKM QEEEPQKKLPVTFEDKRKANYERGNMELEKRRQALMEQQQREAERKAQKEKEEWERKQRELQEQEWKKQL ELEKRLEKQRELERQREEERRKDIERREAAKQELERQRRLEWERIRRQELLNQKNREQEEIVRLNSKKKN LHLELEALNGKHQQISGRLQDVRLKKQTQKTELEVLDKQCDLEIMEIKQLQQELQEYQNKLIYLVPEKQL LNERIKNMQFSNTPDSGVSLLHKKSLEKEELCQRLKEQLDALEKETASKLSEMDSFNNQLKCGNMDDSVL QCLLSLLSCLNNLFLLLKELRETYNTQQLALEQLYKIKRDKLKEIERKRLELMQKKKLEDEAARKAKQGK ENLWKENLRKEEEEKQKRLQEEKTQEKIQEEERKAEEKQRKDKDTLKAEEKKRETASVLVNYRALYPFEA RNHDEMSFNSGDIIQVDEKTVGEPGWLYGSFQGNFGWFPCNYVEKMPSSENEKAVSPKKALLPPTVSLSA TSTSSEPLSSNQPASVTDYQNVSFSNLTVNTSWQKKSAFTRTVSPGSVSPIHGQGQVVENLKAQALCSWT AKKDNHLNFSKHDIITVLEQQENWWFGEVHGGRGWFPKSYVKIIPGSEVKREEPEALYAAVNKKPTSAAY SVGEEYIALYPYSSVEPGDLTFTEGEEILVTQKDGEWWTGSIGDRSGIFPSNYVKPKDQESFGSASKSGA SNKKPEIAQVTSAYVASGSEQLSLAPGQLILILKKNTSGWWQGELQARGKKRQKGWFPASHVKLLGPSSE RATPAFHPVCQVIAMYDYAANNEDELSFSKGQLINVMNKDDPDWWQGEINGVTGLFPSNYVKMTTDSDPS QQWCADLQTLDTMQPIERKRQGYIHELIQTEERYMADLQLVVEVRRLLLASSRGICCLS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_147152 |
ORF Size | 3747 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_147152.3 |
RefSeq ORF | 3750 bp |
Locus ID | 50618 |
UniProt ID | Q9NZM3 |
Cytogenetics | 2p23.3 |
Domains | EH, SH3, EFh |
Protein Families | Druggable Genome |
MW | 141.6 kDa |
Gene Summary | This gene encodes a cytoplasmic protein which contains SH3 domains. This protein is a member of a family of proteins involved in clathrin-mediated endocytosis. Intersectin 2 is thought to regulate the formation of clathrin-coated vesicles and also may function in the induction of T cell antigen receptor (TCR) endocytosis. [provided by RefSeq, Jan 2017] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC229421L3 | Lenti-ORF clone of ITSN2 (Myc-DDK-tagged)-Human intersectin 2 (ITSN2), transcript variant 2 |
USD 1,496.00 |
|
RC229421L4 | Lenti-ORF clone of ITSN2 (mGFP-tagged)-Human intersectin 2 (ITSN2), transcript variant 2 |
USD 1,496.00 |
|
RG229421 | ITSN2 (tGFP-tagged) - Human intersectin 2 (ITSN2), transcript variant 2 |
USD 1,396.00 |
|
SC309939 | ITSN2 (untagged)-Human intersectin 2 (ITSN2), transcript variant 2 |
USD 1,196.00 |
|
SC328056 | ITSN2 (untagged)-Human intersectin 2 (ITSN2) transcript variant 2 |
USD 1,197.00 |
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