Emi1 (FBXO5) (NM_001142522) Human Tagged Lenti ORF Clone
CAT#: RC227496L3
- LentiORF®
Lenti-ORF clone of FBXO5 (Myc-DDK-tagged)-Human F-box protein 5 (FBXO5), transcript variant 2
"NM_001142522" in other vectors (4)
Product Images
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Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Emi1 |
Synonyms | EMI1; FBX5; Fbxo31 |
Vector | pLenti-C-Myc-DDK-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(RC227496).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
ACCN | NM_001142522 |
ORF Size | 1203 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001142522.1 |
RefSeq Size | 2269 bp |
RefSeq ORF | 1206 bp |
Locus ID | 26271 |
UniProt ID | Q9UKT4 |
Cytogenetics | 6q25.2 |
Protein Families | Druggable Genome |
Protein Pathways | Oocyte meiosis |
MW | 45.4 kDa |
Gene Summary | This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. This protein is similar to xenopus early mitotic inhibitor-1 (Emi1), which is a mitotic regulator that interacts with Cdc20 and inhibits the anaphase promoting complex. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Dec 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC227496 | FBXO5 (Myc-DDK-tagged)-Human F-box protein 5 (FBXO5), transcript variant 2 |
USD 503.00 |
|
RC227496L4 | Lenti-ORF clone of FBXO5 (mGFP-tagged)-Human F-box protein 5 (FBXO5), transcript variant 2 |
USD 803.00 |
|
RG227496 | FBXO5 (tGFP-tagged) - Human F-box protein 5 (FBXO5), transcript variant 2 |
USD 703.00 |
|
SC325052 | FBXO5 (untagged)-Human F-box protein 5 (FBXO5), transcript variant 2 |
USD 503.00 |
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