CD299 (CLEC4M) (NM_001144909) Human Tagged ORF Clone

CAT#: RC227458

CLEC4M (Myc-DDK-tagged)-Human C-type lectin domain family 4, member M (CLEC4M), transcript variant 9. Note: ORF is codon optimized

Product Images

Circular map for RC227458

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CD299
• Synonyms: CD209L; CD299; DC-SIGN2; DC-SIGNR; DCSIGNR; HP10347; L-SIGN; LSIGN
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC227458 ORF sequence, codon optimized.
  Due to the complexity of NM_001144909, the ORF clone is codon optimized for mammalian Expression.
  The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.
  
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTGACTCTAAAGAGCCCAGAGTGCAACAGTTGGGCCTTCTTGAGGAGGACCCAACCACCAGCGGGA
  TTAGACTTTTCCCAAGGGATTTTCAGTTTCAGCAGATCCATGGACATAAGAGCTCAACGGGATGCCTTGG
  TCACGGCGCTCTTGTCCTCCAACTGCTGTCTTTTATGCTGCTGGCGGGCGTGCTCGTGGCCATTCTTGTT
  CAAGTTTCTAAAGTGCCTTCATCACTGTCTCAGGAGCAGTCAGAGCAGGACGCTATCTATCAAAACCTTA
  CCCAGTTGAAAGCCGCTGTTGGAGAACTGTCCGAAAAGTCCAAGTTGCAGGAGATCTATCAGGAGCTCAC
  ACAGCTGAAAGCTGCTGTTGGCGAGCTCCCCGAGAAAAGCAAGCTCCAAGAGATTTATCAGGAGCTGACA
  AGGCTGAAGGCTGCAGTGGGAGAGCTGCCAGAGAAAAGTAAGTTGCAGGAGATTTATCAAGAACTGACTA
  GGCTTAAAGCCGCTGTCGGCGAGCTGCCTGAGAAGAGCAAATTGCAGGAAATTTACCAAGAACTTACACA
  GCTTAAGGCTGCAGTCGGCGAACTCCCGGACCAGTCCAAACAGCAACAGATTTACCAGGAATTGACTGAC
  CTGAAAACCGCCTTCGAAAGGCTGTGCAGACATTGCCCAAAGGATTGGACTTTCTTCCAGGGAAATTGTT
  ATTTCATGAGTAATAGTCAGAGAAATTGGCACGATTCAGTGACGGCCTGTCAGGAAGTTAGGGCGCAGCT
  TGTGGTTATTAAAACCGCCGAGGAGCAGAACTTCCTGCAGCTGCAGACTAGTCGGTCCAACAGATTCAGT
  TGGATGGGCTTGAGCGATCTCAACCAGGAGGGCACTTGGCAGTGGGTGGACGGGAGCCCCTTGAGTCCGT
  CATTTCAGAGATATTGGAATTCTGGGGAGCCGAATAACAGCGGCAACGAGGATTGCGCCGAGTTCTCTGG
  CAGCGGGTGGAACGACAATCGCTGCGACGTGGACAACTATTGGATTTGTAAGAAGCCCGCCGCCTGTTTT
  AGAGATGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC227458 representing NM_001144909
  Red=Cloning site Green=Tags(s)
  
  MSDSKEPRVQQLGLLEEDPTTSGIRLFPRDFQFQQIHGHKSSTGCLGHGALVLQLLSFMLLAGVLVAILV
  QVSKVPSSLSQEQSEQDAIYQNLTQLKAAVGELSEKSKLQEIYQELTQLKAAVGELPEKSKLQEIYQELT
  RLKAAVGELPEKSKLQEIYQELTRLKAAVGELPEKSKLQEIYQELTQLKAAVGELPDQSKQQQIYQELTD
  LKTAFERLCRHCPKDWTFFQGNCYFMSNSQRNWHDSVTACQEVRAQLVVIKTAEEQNFLQLQTSRSNRFS
  WMGLSDLNQEGTWQWVDGSPLSPSFQRYWNSGEPNNSGNEDCAEFSGSGWNDNRCDVDNYWICKKPAACF
  RDE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001144909
• ORF Size: 1059 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001144909.1, NP_001138381.1
• RefSeq Size: 1838 bp
• RefSeq ORF: 1062 bp
• Locus ID: 10332
• UniProt ID: Q9H2X3
• Cytogenetics: 19p13.2
• Protein Families: Druggable Genome, Transmembrane
• MW: 40.1 kDa
• Gene Summary: This gene encodes a C-type lectin that functions in cell adhesion and pathogen recognition. This receptor recognizes a wide range of evolutionarily divergent pathogens with a large impact on public health, including tuberculosis mycobacteria, and viruses including Ebola, hepatitis C, HIV-1, influenza A, West Nile virus and the SARS-CoV acute respiratory syndrome coronavirus. The protein is organized into four distinct domains: a C-terminal carbohydrate recognition domain, a flexible tandem-repeat neck domain of variable length, a transmembrane region and an N-terminal cytoplasmic domain involved in internalization. This gene is closely related in terms of both sequence and function to a neighboring gene, CD209 (Gene ID: 30835), also known as DC-SIGN. The two genes differ in viral recognition and expression patterns, with this gene showing high expression in endothelial cells of the liver, lymph node and placenta. Polymorphisms in the tandem repeat neck domain are associated with resistance to SARS infection. [provided by RefSeq, May 2020]