PHF21B (NM_001135862) Human Tagged ORF Clone

CAT#: RC227020

PHF21B (Myc-DDK-tagged)-Human PHD finger protein 21B (PHF21B), transcript variant 2

Product Images

Circular map for RC227020

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PHF21B
• Synonyms: BHC80L; PHF4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC227020 representing NM_001135862
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCTGCAGAGCCGGCCCGAGGCGCTCGCCGTGGAACTCGCGCGCCACCAGAACGGCGACCTCAAGA
  AGCAGCTCCACGAAAGGCAGCCGCGGATCGCCGCGCTCAGCGACAAACAAGCTTTGGGAACGATCACTGC
  AGTGCCTGTCACGGGTCCTCAGGTCAGCTCCTTGCAGAGGTTGGCCGGGCAAGGAGCGGCAGTGCTACCT
  CAGGTTAGGCCAAAGACTCTGATTCCAGACAGCCTCCCCGTTGCCCCGGGCCGGGACCGGCCACCCAAGC
  AGCCCCCAACATTCCAGAAGGCCACCGTGGTCAGCGTCAAGAACCCCAGCCCAGCCCTCCCCACCGCCAA
  CAACACTGTCAGCCATGTGCCAGCGCCCGGCAGCCAGCCCCAGGCCCTCGCCGAGCCCGCCGCCCTCGCC
  TCTCCGCTGAGCAGTGCGGGGGTGGCCTACGCCATCATCTCCACCTCCCCCAGCAATGCCGCCGCCATGG
  CCCCCAGCACCGCCGTGTCTGTGGTCAGTGACAGCATCAAAGTCCAGCCCCTCCTCATCAGTGCTGACAA
  CAAGGTCATCATCATTCAGCCTCAAGTGCAGACGCAGCCCGAGAGCACGGCAGAGTCGCGGCCGCCCACA
  GAGGAGCCATCTCAGGGAGCTCAGGCCACCAAAAAGAAGAAGGAAGACCGGCCCCCGACCCAGGAGAACC
  CCGAGAAAATCGCCTTCATGGTAGCGCTAGGCCTGGTTACCACGGAACATTTGGAAGAAATCCAGAGCAA
  GCGACAGGAGCGGAAGAGAAGAAGCACAGCCAACCCTGCCTACAGCGGCCTCCTGGAGACCGAGAGGAAA
  CGGCTGGCCTCCAACTATCTCAACAACCCCCTGTTCCTCACAGCGAGAGCCAATGAGGACCCCTGCTGGA
  AGAACGAGATCACCCACGATGAGCACTGTGCCGCCTGCAAGCGAGGGGCCAACCTGCAGCCCTGCGGCAC
  CTGCCCGGGGGCCTACCACCTCAGCTGCCTGGAGCCGCCCCTCAAGACGGCGCCCAAGGGCGTGTGGGTG
  TGCCCCAGGTGCCAGCAGAAGGCCTTAAAGAAAGACGAGGGTGTGCCCTGGACTGGGATGCTGGCCATCG
  TGCACTCTTATGTCACCCACAAGACAGTCAAAGAAGAGGAGAAGCAGAAGCTGCTGCAACGAGGCAGTGA
  GCTGCAGAACGAGCACCAGCAGCTGGAGGAGCGGGACCGGCGGCTGGCGTCAGCAGTGCAGAAATGCCTG
  GAGTTGAAGACAAGCCTGCTGGCCCGCCAGAGGGGCACCCAGTCATCCCTGGACCGCCTGCGGGCCCTCC
  TGAGACTGATACAGGGCGAGCAGCTGCTCCAGGTCACCATGACGACCACTAGCCCTGCCCCACTGCTGGC
  CGGGCCCTGGACCAAGCCCTCAGTGGCAGCCACACACCCCACCGTCCAGCACCCCCAGGGCCACAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC227020 representing NM_001135862
  Red=Cloning site Green=Tags(s)
  
  MELQSRPEALAVELARHQNGDLKKQLHERQPRIAALSDKQALGTITAVPVTGPQVSSLQRLAGQGAAVLP
  QVRPKTLIPDSLPVAPGRDRPPKQPPTFQKATVVSVKNPSPALPTANNTVSHVPAPGSQPQALAEPAALA
  SPLSSAGVAYAIISTSPSNAAAMAPSTAVSVVSDSIKVQPLLISADNKVIIIQPQVQTQPESTAESRPPT
  EEPSQGAQATKKKKEDRPPTQENPEKIAFMVALGLVTTEHLEEIQSKRQERKRRSTANPAYSGLLETERK
  RLASNYLNNPLFLTARANEDPCWKNEITHDEHCAACKRGANLQPCGTCPGAYHLSCLEPPLKTAPKGVWV
  CPRCQQKALKKDEGVPWTGMLAIVHSYVTHKTVKEEEKQKLLQRGSELQNEHQQLEERDRRLASAVQKCL
  ELKTSLLARQRGTQSSLDRLRALLRLIQGEQLLQVTMTTTSPAPLLAGPWTKPSVAATHPTVQHPQGHN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001135862
• ORF Size: 1467 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001135862.3
• RefSeq ORF: 1470 bp
• Locus ID: 112885
• UniProt ID: Q96EK2
• Cytogenetics: 22q13.31
• Protein Families: Druggable Genome, Transcription Factors
• MW: 52.9 kDa