ATF7 (NM_001130060) Human Tagged ORF Clone
CAT#: RC225780
- TrueORF®
ATF7 (Myc-DDK-tagged)-Human activating transcription factor 7 (ATF7), transcript variant 3
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_001130060" in other vectors (4)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | ATF7 |
Synonyms | ATFA |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC225780 representing NM_001130060
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAGACGACAGACCGTTTGTGTGCAATGCCCCGGGCTGTGGACAGAGATTTACAAACGAGGACCACC TGGCAGTTCATAAACACAAGCATGAGATGACATTGAAATTTGGCCCAGCCCGAACTGACTCAGTCATCAT TGCAGATCAAACGCCTACTCCAACTAGATTCCTGAAGAACTGTGAGGAGGTGGGACTCTTCAATGAACTA GCTAGCTCCTTTGAACATGAATTCAAGAAAGCTGCAGATGAGGATGAGAAAAAGGCTGCTGCTGGGCCCC TTGACATGTCTCTGCCTTCCACACCAGACATCAAAATCAAAGAAGAAGAGCCAGTGGAGGAGGTTACCCC AAAGCCTGTTCTGATCTCTACCCCCACACCCACCATTGTACGTCCTGGCTCCCTGCCTCTCCACTTGGGC TATGATCCACTTCATCCAACCCTTCCCTCCCCAACCTCTGTCATCACACAGGCTCCACCATCCAACAGGC AAATGGGGTCTCCCACTGGCTCCCTCCCTCTTGTCATGCATCTTGCTAATGGACAGACCATGCCTGTGTT GCCAGGGCCTCCAGTACAGATGCCGTCTGTTATATCGCTGGCCAGACCTGTGTCCATGGTGCCCAACATT CCTGGTATCCCTGGCCCACCAGTTAACAGTAGTGGCTCCATTTCTCCCTCTGGCCACCCTATACCATCAG AAGCCAAGATGAGACTGAAAGCCACCCTAACTCACCAAGTCTCCTCAATCAATGGTGGTTGTGGAATGGT GGTGGGTACTGCCAGCACCATGGTGACAGCCCGCCCAGAGCAGAGCCAGATTCTCATCCAGCACCCTGAT GCCCCATCCCCTGCCCAGCCACAGGTCTCACCAGCTCAGCCCACCCCTAGTACTGGGGGGCGACGGCGGC GCACAGTAGATGAAGATCCAGATGAGCGACGGCAGCGCTTTCTGGAGCGCAACCGGGCTGCAGCCTCCCG CTGCCGCCAAAAGCGAAAGCTGTGGGTGTCCTCCCTAGAGAAGAAGGCCGAAGAACTCACTTCTCAGAAC ATTCAGCTGAGTAATGAAGTCACATTACTACGCAATGAGGTGGCCCAGTTGAAACAGCTACTGTTAGCTC ATAAAGACTGCCCAGTCACTGCACTACAGAAAAAGACTCAAGGCTATTTAGAAAGCCCCAAGGAAAGCTC AGAGCCAACGGGTTCTCCAGCCCCTGTGATTCAGCACAGCTCAGCAACAGCCCCTAGCAATGGCCTCAGT GTTCGCTCTGCAGCTGAAGCTGTGGCCACCTCGGTCCTCACTCAGATGGCCAGCCAAAGGACAGAACTGA GCATGCCGATACAATCGCATGTAATCATGACCCCACAGTCCCAGTCTGCGGGCAGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC225780 representing NM_001130060
Red=Cloning site Green=Tags(s) MGDDRPFVCNAPGCGQRFTNEDHLAVHKHKHEMTLKFGPARTDSVIIADQTPTPTRFLKNCEEVGLFNEL ASSFEHEFKKAADEDEKKAAAGPLDMSLPSTPDIKIKEEEPVEEVTPKPVLISTPTPTIVRPGSLPLHLG YDPLHPTLPSPTSVITQAPPSNRQMGSPTGSLPLVMHLANGQTMPVLPGPPVQMPSVISLARPVSMVPNI PGIPGPPVNSSGSISPSGHPIPSEAKMRLKATLTHQVSSINGGCGMVVGTASTMVTARPEQSQILIQHPD APSPAQPQVSPAQPTPSTGGRRRRTVDEDPDERRQRFLERNRAAASRCRQKRKLWVSSLEKKAEELTSQN IQLSNEVTLLRNEVAQLKQLLLAHKDCPVTALQKKTQGYLESPKESSEPTGSPAPVIQHSSATAPSNGLS VRSAAEAVATSVLTQMASQRTELSMPIQSHVIMTPQSQSAGR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001130060 |
ORF Size | 1386 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001130060.2 |
RefSeq ORF | 1389 bp |
Locus ID | 11016 |
UniProt ID | P17544 |
Cytogenetics | 12q13.13 |
Protein Families | Transcription Factors |
MW | 49.5 kDa |
Gene Summary | Plays important functions in early cell signaling. Binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AG][AG]-3'), a sequence present in many viral and cellular promoters. Activator of the NF-ELAM1/delta-A site of the E-selectin promoter. Has no intrinsic transcriptional activity, but activates transcription on formation of JUN or FOS heterodimers. Also can bind TRE promoter sequences when heterodimerized with members of the JUN family.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC225780L3 | Lenti ORF clone of Human activating transcription factor 7 (ATF7), transcript variant 3, Myc-DDK-tagged |
USD 757.00 |
|
RC225780L4 | Lenti ORF clone of Human activating transcription factor 7 (ATF7), transcript variant 3, mGFP tagged |
USD 757.00 |
|
RG225780 | ATF7 (tGFP-tagged) - Human activating transcription factor 7 (ATF7), transcript variant 3 |
USD 657.00 |
|
SC323081 | ATF7 (untagged)-Human activating transcription factor 7 (ATF7), transcript variant 3 |
USD 503.00 |
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