SYNPR (NM_001130003) Human Tagged ORF Clone

CAT#: RC225395

SYNPR (Myc-DDK-tagged)-Human synaptoporin (SYNPR), transcript variant 1

Product Images

Circular map for RC225395

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  Western blot validation of overexpression lysate (Cat# LY427094) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC225395 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified SYNPR protein (Cat# TP325395). The protein was produced from HEK293T cells transfected with SYNPR cDNA clone (Cat# RC225395) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SYNPR
• Synonyms: SPO
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC225395 representing NM_001130003
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACCCTGTGAGTCAGCTGGCCTCTGCGGGCACCTTCCGGGTGCTGAAGGAGCCCCTTGCCTTCCTGC
  GAGCCCTGGAATTGCTTTTTGCAATCTTTGCATTTGCAACATGCGGTGGCTATTCTGGAGGCCTGCGGCT
  GAGTGTGGACTGCGTCAACAAGACAGAAAGTAACCTCAGCATCGACATAGCGTTTGCCTACCCATTCAGG
  TTGCACCAGGTGACGTTTGAGGTGCCCACCTGCGAGGGAAAGGAACGGCAGAAGCTGGCATTGATTGGTG
  ACTCCTCGTCTTCAGCAGAGTTCTTCGTCACTGTTGCTGTCTTCGCCTTCCTCTACTCTTTGGCTGCCAC
  TGTCGTTTACATTTTCTTCCAGAACAAATACCGGGAAAACAACCGGGGCCCACTCATTGACTTCATTGTC
  ACTGTAGTCTTTTCGTTCTTGTGGTTGGTGGGTTCATCAGCTTGGGCAAAAGGACTGTCTGACGTCAAAG
  TTGCAACGGATCCCAAGGAAGTATTGCTACTAATGTCAGCTTGCAAACAGCCATCCAACAAATGCATGGC
  TATCCACAGCCCTGTTATGTCAAGCTTAAACACTTCTGTGGTCTTTGGATTCTTGAACTTTATTCTCTGG
  GCTGGAAACATATGGTTTGTTTTCAAGGAGACCGGCTGGCATTCTTCGGGACAGAGATATCTTTCAGATC
  CAATGGAGAAGCACTCCAGCAGCTATAATCAAGGTGGTTACAACCAAGACAGCTATGGATCAAGCAGTGG
  GTACAGTCAGCAGGCGAGTTTGGGGCCAACCTCAGATGAGTTTGGCCAACAGCCTACTGGCCCCACTTCC
  TTTACCAATCAGATT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC225395 representing NM_001130003
  Red=Cloning site Green=Tags(s)
  
  MDPVSQLASAGTFRVLKEPLAFLRALELLFAIFAFATCGGYSGGLRLSVDCVNKTESNLSIDIAFAYPFR
  LHQVTFEVPTCEGKERQKLALIGDSSSSAEFFVTVAVFAFLYSLAATVVYIFFQNKYRENNRGPLIDFIV
  TVVFSFLWLVGSSAWAKGLSDVKVATDPKEVLLLMSACKQPSNKCMAIHSPVMSSLNTSVVFGFLNFILW
  AGNIWFVFKETGWHSSGQRYLSDPMEKHSSSYNQGGYNQDSYGSSSGYSQQASLGPTSDEFGQQPTGPTS
  FTNQI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001130003
• ORF Size: 855 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001130003.2
• RefSeq ORF: 858 bp
• Locus ID: 132204
• UniProt ID: Q8TBG9
• Cytogenetics: 3p14.2
• Protein Families: Secreted Protein, Transmembrane
• MW: 31.1 kDa
• Gene Summary: Intrinsic membrane protein of small synaptic vesicles. Probable vesicular channel protein (By similarity).[UniProtKB/Swiss-Prot Function]