Plexin B1 (PLXNB1) (NM_002673) Human Tagged ORF Clone
CAT#: RC224819
PLXNB1 (Myc-DDK-tagged)-Human plexin B1 (PLXNB1), transcript variant 1
ORF Plasmid: tGFP
"NM_002673" in other vectors (2)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Plexin B1 |
Synonyms | PLEXIN-B1; PLXN5; SEP |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC224819 representing NM_002673
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTGCTCTGGGCCCAGCTCTTCTCCAGGCTCTCTGGGCCGGGTGGGTCCTCACCCTCCAGCCCCTTC CACCAACTGCATTCACTCCCAATGGCACGTATCTGCAGCACCTGGCAAGGGACCCCACCTCAGGCACCCT CTACCTGGGGGCTACCAACTTCCTGTTCCAGCTGAGCCCTGGGCTGCAGCTGGAGGCCACAGTGTCCACC GGCCCTGTGCTAGACAGCAGGGACTGCCTGCCACCTGTGATGCCTGATGAGTGCCCCCAGGCCCAGCCTA CCAACAACCCGAATCAGCTGCTCCTGGTGAGCCCAGGGGCCCTGGTGGTATGCGGGAGCGTGCACCAGGG GGTCTGTGAACAGCGGCGCCTGGGGCAGCTCGAGCAGCTGCTGCTGCGGCCAGAGCGGCCTGGGGACACA CAATATGTGGCTGCCAATGATCCTGCGGTCAGCACGGTGGGGCTGGTAGCCCAGGGCTTGGCAGGGGAGC CCCTCCTGTTTGTGGGGCGAGGATACACCAGCAGGGGTGTGGGGGGTGGCATTCCACCCATCACAACCCG GGCCCTGTGGCCGCCCGACCCCCAAGCTGCCTTCTCCTATGAGGAGACAGCCAAGCTGGCAGTGGGCCGC CTCTCCGAGTACAGCCACCACTTCGTGAGTGCCTTTGCACGTGGGGCCAGCGCCTACTTCCTGTTCCTGC GGCGGGACCTGCAGGCTCAGTCTAGAGCTTTTCGTGCCTATGTATCTCGAGTGTGTCTCCGGGACCAGCA CTACTACTCCTATGTGGAGTTGCCTCTGGCCTGCGAAGGTGGCCGCTACGGGCTGATCCAGGCTGCAGCT GTGGCCACGTCCAGGGAGGTGGCGCATGGGGAGGTGCTCTTTGCAGCTTTCTCCTCGGCTGCACCCCCCA CTGTGGGCCGGCCCCCATCGGCGGCTGCTGGGGCATCTGGAGCCTCTGCCCTCTGTGCCTTCCCCCTGGA TGAGGTGGACCGGCTTGCTAATCGCACGCGAGATGCCTGCTACACCCGGGAGGGTCGTGCTGAGGATGGG ACCGAGGTGGCCTACATCGAGTATGATGTCAATTCTGACTGTGCACAGCTGCCAGTGGACACCCTGGATG CTTATCCCTGTGGCTCAGACCACACGCCCAGCCCCATGGCCAGCCGGGTCCCGCTGGAAGCCACACCAAT TCTGGAGTGGCCAGGGATTCAGCTAACAGCTGTGGCAGTCACCATGGAAGATGGACACACCATCGCTTTC CTGGGTGATAGTCAAGGGCAGCTGCACAGGGTCTACTTGGGCCCAGGGAGCGATGGCCACCCATACTCCA CACAGAGCATCCAGCAGGGGTCTGCAGTGAGCAGAGACCTCACCTTTGATGGGACCTTTGAGCACCTGTA TGTCATGACCCAGAGCACACTTCTGAAGGTTCCTGTGGCTTCCTGTGCTCAGCACCTGGACTGTGCATCT TGCCTTGCTCACAGGGACCCATACTGTGGGTGGTGCGTGCTCCTTGGCAGGTGCAGTCGCCGTTCTGAGT GCTCGAGGGGCCAGGGCCCAGAGCAGTGGCTATGGAGCTTCCAGCCTGAGCTGGGCTGTCTGCAAGTGGC AGCCATGAGTCCTGCCAACATCAGCCGAGAGGAGACGAGGGAGGTTTTCCTATCAGTGCCAGACCTGCCA CCCCTGTGGCCAGGGGAGTCATATTCCTGCCACTTTGGGGAACATCAGAGTCCTGCCCTGCTGACTGGTT CTGGTGTGATGTGCCCCTCCCCAGACCCTAGTGAGGCCCCAGTGCTGCCGAGAGGAGCCGACTACGTATC CGTGAGCGTGGAGCTCAGATTTGGCGCTGTTGTGATCGCCAAAACTTCCCTCTCTTTCTATGACTGTGTG GCGGTCACTGAACTCCGCCCATCTGCGCAGTGCCAGGCCTGTGTGAGCAGCCGCTGGGGGTGTAACTGGT GTGTCTGGCAGCACCTGTGCACCCACAAGGCCTCGTGTGATGCTGGGCCCATGGTTGCAAGCCATCAGAG CCCGCTTGTCTCCCCAGACCCTCCTGCAAGAGGTGGACCCAGCCCCTCCCCACCCACAGCCCCCAAAGCC CTGGCCACCCCTGCTCCTGACACCCTTCCCGTGGAGCCTGGGGCTCCCTCCACAGCCACAGCTTCGGACA TCTCACCTGGGGCTAGTCCTTCCCTGCTCAGCCCCTGGGGGCCATGGGCAGGTTCTGGCTCCATATCTTC CCCTGGCTCCACAGGGTCGCCTCTCCATGAGGAGCCCTCCCCTCCCAGCCCCCAAAATGGACCTGGAACC GCTGTCCCTGCCCCCACTGACTTCAGACCCTCAGCCACACCTGAGGACCTCTTGGCCTCCCCGCTGTCAC CGTCAGAGGTAGCAGCAGTGCCCCCTGCAGACCCTGGCCCCGAGGCTCTTCATCCCACAGTGCCCCTGGA CCTGCCCCCTGCCACTGTTCCTGCCACCACTTTCCCAGGGGCCATGGGCTCCGTGAAGCCCGCCCTGGAC TGGCTCACGAGAGAAGGCGGCGAGCTGCCCGAGGCGGACGAGTGGACGGGGGGTGACGCACCCGCCTTCT CCACTTCCACCCTCCTCTCAGGTGATGGAGACTCAGCAGAGCTTGAGGGCCCTCCCGCCCCCCTCATCCT CCCGTCCAGCCTCGACTACCAGTATGACACCCCCGGGCTCTGGGAGCTGGAAGAGGCGACCTTGGGGGCA AGCTCCTGCCCCTGTGTGGAGAGCGTTCAGGGCTCCACGTTGATGCCGGTCCATGTGGAGCGGGAAATCC GGCTGCTAGGCAGGAACCTGCACCTTTTCCAGGATGGCCCAGGAGACAATGAGTGTGTGATGGAGCTGGA GGGCCTCGAGGTGGTGGTTGAGGCCCGGGTCGAGTGTGAGCCACCTCCAGATACCCAGTGCCATGTCACC TGCCAGCAGCACCAGCTCAGCTATGAGGCTCTGCAGCCGGAGCTCCGTGTGGGGCTGTTTCTGCGTCGGG CCGGCCGTCTGCGTGTGGACAGTGCTGAGGGGCTGCATGTGGTACTGTATGACTGTTCCGTGGGACATGG AGACTGCAGCCGCTGCCAAACTGCCATGCCCCAGTATGGCTGTGTGTGGTGTGAGGGGGAGCGTCCACGT TGTGTGACCCGGGAGGCCTGTGGTGAGGCTGAGGCTGTGGCCACCCAGTGCCCAGCGCCCCTCATCCACT CGGTGGAGCCACTGACTGGGCCTGTAGACGGAGGCACCCGTGTCACCATCAGGGGCTCCAACCTGGGCCA GCATGTGCAGGATGTGCTGGGCATGGTCACGGTGGCTGGAGTGCCCTGTGCTGTGGATGCCCAGGAGTAC GAGGTCTCCAGCAGCCTCGTGTGCATCACCGGGGCCAGTGGGGAGGAGGTGGCCGGCGCCACAGCGGTGG AGGTGCCGGGAAGAGGACGTGGTGTCTCAGAACACGACTTTGCCTACCAGGATCCGAAGGTCCATTCCAT CTTCCCGGCCCGCGGCCCCAGAGCTGGGGGCACCCGTCTCACCCTGAATGGCTCCAAGCTCCTGACTGGG CGGCTGGAGGACATCCGAGTGGTGGTTGGAGACCAGCCTTGTCACTTGCTGCCGGAGCAGCAGTCAGAAC AACTGCGGTGTGAGACCAGCCCACGCCCCACGCCTGCCACGCTCCCTGTGGCTGTGTGGTTTGGGGCCAC GGAGCGGAGGCTTCAACGCGGACAGTTCAAGTATACCTTGGACCCCAACATCACCTCTGCTGGCCCCACC AAGAGCTTCCTCAGTGGAGGACGTGAGATATGCGTCCGTGGCCAGAATCTGGACGTGGTACAGACGCCAA GAATCCGGGTGACCGTGGTCTCGAGAATGCTGCAGCCCAGCCAGGGGCTTGGACGGAGGCGTCGCGTGGT CCCGGAGACGGCATGTTCCCTTGGACCCTCCTGCAGTAGCCAGCAATTTGAGGAGCCGTGCCATGTCAAC TCCTCCCAGCTCATCACGTGCCGCACACCTGCCCTCCCAGGCCTGCCTGAGGACCCCTGGGTCCGGGTGG AATTTATCCTTGACAACCTGGTCTTTGACTTTGCAACACTGAACCCCACACCTTTCTCCTATGAGGCCGA CCCCACCCTGCAGCCACTCAACCCTGAGGACCCCACCATGCCATTCCGGCACAAGCCTGGGAGTGTGTTC TCCGTGGAGGGGGAGAACCTGGACCTTGCAATGTCCAAGGAGGAGGTGGTGGCTATGATAGGGGATGGCC CCTGTGTGGTGAAGACGCTGACGCGGCACCACCTGTACTGCGAGCCCCCCGTGGAGCAGCCCCTGCCACG GCACCATGCCCTCCGAGAGGCACCTGACTCTTTGCCTGAGTTCACGGTGCAGATGGGGAACTTGCGCTTC TCCCTGGGTCACGTGCAGTATGACGGCGAGAGCCCTGGGGCTTTTCCTGTGGCAGCCCAGGTGGGCTTGG GGGTGGGCACCTCTCTTCTGGCTCTGGGTGTCATCATCATTGTCCTCATGTACAGGAGGAAGAGCAAGCA GGCCCTGAGGGACTATAAGAAGGTTCAGATCCAGCTGGAGAATCTGGAGAGCAGTGTGCGGGACCGCTGC AAGAAGGAATTCACAGACCTCATGACTGAGATGACCGATCTCACCAGTGACCTCCTGGGCAGCGGCATCC CCTTCCTCGACTACAAGGTGTATGCGGAGAGGATCTTCTTCCCTGGGCACCGCGAGTCGCCCTTGCACCG GGACCTGGGTGTGCCTGAGAGCAGACGGCCCACTGTGGAGCAAGGGCTGGGGCAGCTCTCTAACCTGCTC AACAGCAAGCTCTTCCTCACCAAGTTCATCCACACGCTGGAGAGCCAGCGCACCTTTTCAGCTCGGGACC GTGCCTACGTGGCATCTCTGCTCACCGTGGCACTGCATGGGAAGCTTGAGTATTTCACTGACATCCTCCG CACTCTGCTCAGTGACCTGGTTGCCCAGTATGTGGCCAAGAACCCCAAGCTGATGCTGCGCAGGACAGAG ACTGTGGTGGAGAAGCTGCTCACCAACTGGATGTCCATCTGTCTGTATACCTTCGTGAGGGACTCCGTAG GGGAGCCTCTGTACATGCTCTTTCGAGGGATTAAGCACCAAGTGGATAAGGGGCCAGTGGACAGTGTGAC AGGCAAGGCCAAATACACCTTGAACGACAACCGCCTGCTCAGAGAGGATGTGGAGTACCGTCCCCTGACC TTGAATGCACTATTGGCTGTGGGGCCTGGGGCAGGAGAGGCCCAGGGCGTGCCCGTGAAGGTCCTAGACT GTGACACCATCTCCCAGGCAAAGGAGAAGATGCTGGACCAGCTTTATAAAGGAGTGCCTCTCACCCAGCG GCCAGACCCTCGCACCCTTGATGTTGAGTGGCGGTCTGGGGTGGCCGGGCACCTCATTCTTTCTGACGAG GATGTCACTTCTGAGGTCCAGGGTCTGTGGAGGCGCCTGAACACACTGCAGCATTACAAGGTCCCAGATG GAGCAACTGTGGCCCTCGTCCCCTGCCTCACCAAGCATGTGCTCCGGGAAAACCAGGATTATGTCCCTGG AGAGCGGACCCCAATGCTGGAGGATGTAGATGAGGGGGGCATCCGGCCCTGGCACCTGGTGAAGCCAAGT GATGAGCCGGAGCCGCCCAGGCCTCGGAGGGGCAGCCTTCGGGGCGGGGAGCGTGAGCGCGCCAAGGCCA TCCCTGAGATCTACCTGACCCGCCTGCTGTCCATGAAGGGCACCCTGCAGAAGTTCGTGGATGACCTGTT CCAGGTGATTCTCAGCACCAGCCGCCCCGTGCCGCTCGCTGTGAAGTACTTCTTTGACCTGCTGGATGAG CAGGCCCAGCAGCATGGCATCTCCGACCAGGACACCATCCACATCTGGAAGACCAACAGCTTGCCTCTGA GGTTCTGGATCAATATAATAAAAAACCCGCAGTTTGTGTTCGACGTGCAAACATCTGATAACATGGATGC GGTGCTCCTTGTCATTGCACAGACCTTCATGGACGCCTGCACCCTGGCCGACCACAAGCTGGGCCGGGAC TCCCCGATCAACAAACTTCTGTATGCACGGGACATTCCCCGGTACAAGCGGATGGTGGAAAGGTACTATG CAGACATCAGACAGACTGTCCCAGCCAGCGACCAAGAGATGAACTCTGTCCTGGCTGAACTGTCCTGGAA CTACTCCGGAGACCTCGGGGCGCGAGTGGCCCTGCATGAACTCTACAAGTACATCAACAAGTACTATGAC CAGATCATCACTGCCCTGGAGGAGGATGGCACGGCCCAGAAGATGCAGCTGGGCTATCGGCTCCAGCAGA TTGCAGCTGCTGTGGAAAACAAGGTCACAGATCTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC224819 representing NM_002673
Red=Cloning site Green=Tags(s) MPALGPALLQALWAGWVLTLQPLPPTAFTPNGTYLQHLARDPTSGTLYLGATNFLFQLSPGLQLEATVST GPVLDSRDCLPPVMPDECPQAQPTNNPNQLLLVSPGALVVCGSVHQGVCEQRRLGQLEQLLLRPERPGDT QYVAANDPAVSTVGLVAQGLAGEPLLFVGRGYTSRGVGGGIPPITTRALWPPDPQAAFSYEETAKLAVGR LSEYSHHFVSAFARGASAYFLFLRRDLQAQSRAFRAYVSRVCLRDQHYYSYVELPLACEGGRYGLIQAAA VATSREVAHGEVLFAAFSSAAPPTVGRPPSAAAGASGASALCAFPLDEVDRLANRTRDACYTREGRAEDG TEVAYIEYDVNSDCAQLPVDTLDAYPCGSDHTPSPMASRVPLEATPILEWPGIQLTAVAVTMEDGHTIAF LGDSQGQLHRVYLGPGSDGHPYSTQSIQQGSAVSRDLTFDGTFEHLYVMTQSTLLKVPVASCAQHLDCAS CLAHRDPYCGWCVLLGRCSRRSECSRGQGPEQWLWSFQPELGCLQVAAMSPANISREETREVFLSVPDLP PLWPGESYSCHFGEHQSPALLTGSGVMCPSPDPSEAPVLPRGADYVSVSVELRFGAVVIAKTSLSFYDCV AVTELRPSAQCQACVSSRWGCNWCVWQHLCTHKASCDAGPMVASHQSPLVSPDPPARGGPSPSPPTAPKA LATPAPDTLPVEPGAPSTATASDISPGASPSLLSPWGPWAGSGSISSPGSTGSPLHEEPSPPSPQNGPGT AVPAPTDFRPSATPEDLLASPLSPSEVAAVPPADPGPEALHPTVPLDLPPATVPATTFPGAMGSVKPALD WLTREGGELPEADEWTGGDAPAFSTSTLLSGDGDSAELEGPPAPLILPSSLDYQYDTPGLWELEEATLGA SSCPCVESVQGSTLMPVHVEREIRLLGRNLHLFQDGPGDNECVMELEGLEVVVEARVECEPPPDTQCHVT CQQHQLSYEALQPELRVGLFLRRAGRLRVDSAEGLHVVLYDCSVGHGDCSRCQTAMPQYGCVWCEGERPR CVTREACGEAEAVATQCPAPLIHSVEPLTGPVDGGTRVTIRGSNLGQHVQDVLGMVTVAGVPCAVDAQEY EVSSSLVCITGASGEEVAGATAVEVPGRGRGVSEHDFAYQDPKVHSIFPARGPRAGGTRLTLNGSKLLTG RLEDIRVVVGDQPCHLLPEQQSEQLRCETSPRPTPATLPVAVWFGATERRLQRGQFKYTLDPNITSAGPT KSFLSGGREICVRGQNLDVVQTPRIRVTVVSRMLQPSQGLGRRRRVVPETACSLGPSCSSQQFEEPCHVN SSQLITCRTPALPGLPEDPWVRVEFILDNLVFDFATLNPTPFSYEADPTLQPLNPEDPTMPFRHKPGSVF SVEGENLDLAMSKEEVVAMIGDGPCVVKTLTRHHLYCEPPVEQPLPRHHALREAPDSLPEFTVQMGNLRF SLGHVQYDGESPGAFPVAAQVGLGVGTSLLALGVIIIVLMYRRKSKQALRDYKKVQIQLENLESSVRDRC KKEFTDLMTEMTDLTSDLLGSGIPFLDYKVYAERIFFPGHRESPLHRDLGVPESRRPTVEQGLGQLSNLL NSKLFLTKFIHTLESQRTFSARDRAYVASLLTVALHGKLEYFTDILRTLLSDLVAQYVAKNPKLMLRRTE TVVEKLLTNWMSICLYTFVRDSVGEPLYMLFRGIKHQVDKGPVDSVTGKAKYTLNDNRLLREDVEYRPLT LNALLAVGPGAGEAQGVPVKVLDCDTISQAKEKMLDQLYKGVPLTQRPDPRTLDVEWRSGVAGHLILSDE DVTSEVQGLWRRLNTLQHYKVPDGATVALVPCLTKHVLRENQDYVPGERTPMLEDVDEGGIRPWHLVKPS DEPEPPRPRRGSLRGGERERAKAIPEIYLTRLLSMKGTLQKFVDDLFQVILSTSRPVPLAVKYFFDLLDE QAQQHGISDQDTIHIWKTNSLPLRFWINIIKNPQFVFDVQTSDNMDAVLLVIAQTFMDACTLADHKLGRD SPINKLLYARDIPRYKRMVERYYADIRQTVPASDQEMNSVLAELSWNYSGDLGARVALHELYKYINKYYD QIITALEEDGTAQKMQLGYRLQQIAAAVENKVTDL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_002673 |
ORF Size | 6405 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_002673.4 |
RefSeq Size | 7097 bp |
RefSeq ORF | 6408 bp |
Locus ID | 5364 |
UniProt ID | O43157 |
Cytogenetics | 3p21.31 |
Protein Families | Druggable Genome |
Protein Pathways | Axon guidance |
MW | 232.1 kDa |
Gene Summary | Receptor for SEMA4D (PubMed:19843518, PubMed:20877282, PubMed:21912513). Plays a role in GABAergic synapse development (By similarity). Mediates SEMA4A- and SEMA4D-dependent inhibitory synapse development (By similarity). Plays a role in RHOA activation and subsequent changes of the actin cytoskeleton (PubMed:12196628, PubMed:15210733). Plays a role in axon guidance, invasive growth and cell migration (PubMed:12198496).[UniProtKB/Swiss-Prot Function] |
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