RLF (NM_012421) Human Tagged ORF Clone

CAT#: RC223079

RLF (Myc-DDK-tagged)-Human rearranged L-myc fusion (RLF)

Product Images

Circular map for RC223079

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RLF
• Synonyms: ZN-15L; ZNF292L
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC223079 representing NM_012421
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGACGGAAAGGGAGACGCCGCCGCTGTCGCCGGGGCTGGGGCTGAGGCTCCGGCGGTAGCGGGAG
  CCGGAGATGGAGTCGAGACTGAGTCCATGGTTCGGGGTCATCGCCCCGTATCTCCAGCGCCGGGAGCCTC
  GGGACTGCGGCCGTGTCTGTGGCAGCTGGAGACAGAGCTGAGGGAGCAAGAGGTGTCGGAGGTCTCATCT
  TTGAACTACTGCCGGAGCTTCTGCCAGACCTTATTGCAATATGCAAGCAACAAGAATGCATCAGAACATA
  TTGTGTATCTTCTGGAGGTATATCGACTTGCCATCCAAAGCTTTGCCAGTGCACGTCCATACTTAACTAC
  TGAATGTGAAGATGTCCTCTTAGTGCTTGGCAGATTAGTACTGAGTTGTTTCGAATTACTGCTTTCAGTG
  TCTGAAAGTGAACTGCCATGTGAAGTCTGGCTACCATTCCTTCAGTCTCTACAGGAGTCACATGATGCAT
  TATTGGAATTTGGGAATAATAACCTACAAATATTGGTTCATGTTACCAAGGAAGGGGTGTGGAAAAACCC
  AGTTCTTCTTAAAATTCTGTCTCAACAGCCAGTAGAAACGGAGGAAGTCAATAAATTGATTGCACAAGAA
  GGACCTTCCTTTCTGCAAATGCGAATAAAACATTTGTTGAAATCTAACTGCATCCCCCAGGCTACTGCTT
  TATCAAAACTATGTGCAGAATCTAAAGAAATTTCAAATGTGTCATCTTTTCAGCAAGCCTATATCACATG
  TTTATGTTCTATGCTCCCTAATGAAGATGCTATTAAGGAGATTGCAAAGGTCGACTGCAAGGAAGTACTA
  GACATCATTTGTAATCTGGAATCTGAGGGGCAGGATAACACAGCATTTGTTCTTTGTACGACTTACCTTA
  CCCAGCAGCTCCAAACTGCAAGTGTATATTGTTCTTGGGAACTGACTCTTTTTTGGAGTAAACTGCAAAG
  AAGAATTGACCCTTCTTTAGATACTTTTTTGGAGCGCTGTCGTCAGTTTGGTGTCATAGCTAAAACGCAG
  CAGCATTTATTTTGCCTCATTAGAGTTATACAAACTGAAGCACAAGATGCTGGTCTTGGGGTGTCAATTT
  TACTGTGTGTCAGAGCTCTTCAACTCAGATCAAGTGAAGATGAGGAAATGAAGGCATCAGTTTGTAAAAC
  AATTGCCTGTCTTTTACCAGAAGATTTAGAAGTTAGACGAGCCTGTCAGCTTACAGAATTCTTAATTGAA
  CCCAGTTTGGATGGATTTAATATGTTAGAAGAACTATATTTGCAACCAGATCAAAAATTTGATGAAGAAA
  ATGCACCGGTTCCAAATTCTCTTCGATGTGAGCTCTTACTAGCTTTAAAAGCCCACTGGCCTTTTGATCC
  TGAGTTTTGGGACTGGAAAACTTTAAAACGACACTGCCACCAACTTTTAGGACAAGAAGCCTCAGATTCT
  GATGATGATTTAAGTGGCTATGAAATGTCCATTAATGACACAGATGTTTTAGAGTCATTTCTCAGTGACT
  ATGATGAGGGTAAAGAAGATAAACAATATAGAAGAAGAGATTTGACAGATCAGCATAAGGAGAAAAGAGA
  CAAAAAACCTATTGGCTCTTCTGAAAGATATCAGAGGTGGCTTCAGTACAAGTTTTTCTGTTTGTTATGT
  AAGCGGGAATGTATAGAGGCTAGAATTCTTCATCATTCTAAGATGCATATGGAAGATGGAATTTACACCT
  GTCCAGTTTGTATTAAAAAATTTAAGAGAAAAGAAATGTTTGTTCCTCATGTGATGGAGCATGTTAAAAT
  GCCACCAAGCAGAAGGGACCGCTCTAAAAAGAAATTACTGTTAAAAGGCTCTCAAAAGGGTATTTGTCCT
  AAGAGCCCCTCTGCAATCCCAGAGCAAAACCATTCATTGAATGACCAAGCCAAAGGAGAGTCTCATGAAT
  ATGTCACATTCAGCAAATTAGAAGATTGCCACCTGCAAGACAGAGATTTGTATCCATGTCCCGGTACAGA
  CTGTTCCCGTGTGTTTAAGCAATTTAAATACTTAAGTGTGCATCTTAAAGCTGAACACCAAAATAATGAT
  GAAAATGCCAAGCACTACTTGGATATGAAAAATAGAAGAGAGAAGTGTACTTACTGTCGACGACATTTTA
  TGTCTGCTTTTCACCTTCGAGAGCACGAACAAGTGCATTGTGGGCCTCAGCCTTATATGTGTGTATCTAT
  AGATTGCTATGCTAGGTTTGGATCAGTAAATGAACTACTTAACCATAAACAAAAGCATGACGATCTGCGT
  TACAAATGTGAATTAAATGGCTGTAATATTGTTTTCAGTGACTTGGGACAGCTTTACCACCATGAAGCAC
  AACACTTTAGGGATGCATCTTACACATGCAACTTCCTTGGCTGTAAAAAGTTCTATTACTCCAAAATTGA
  ATACCAGAATCACCTCTCAATGCATAATGTTGAAAATTCAAATGGAGACATAAAGAAATCAGTGAAACTT
  GAGGAGTCTGCAACAGGTGAAAAGCAAGATTGTATTAATCAGCCCCATCTACTTAACCAAACTGATAAAT
  CACATTTACCTGAAGATCTTTTCTGTGCAGAATCAGCTAATTCTCAAATAGATACAGAAACTGCAGAAAA
  CCTGAAAGAAAACAGTGACAGTAATTCTAGTGATCAGTTAAGTCATAGCTCTTCAGCTTCAATGAATGAA
  GAGCTAATTGACACACTAGATCACTCTGAAACTATGCAGGATGTATTGTTATCTAATGAGAAAGTCTTTG
  GGCCCTCCAGTTTAAAAGAAAAATGTTCCAGTATGGCAGTTTGTTTTGACGGGACTAAGTTTACCTGTGG
  TTTTGATGGCTGTGGTTCCACATACAAAAATGCAAGAGGAATGCAGAAACATTTACGGAAGGTTCATCCA
  TACCATTTCAAGCCCAAAAAGATAAAGACGAAAGATCTGTTTCCCTCTTTGGGTAATGAACATAATCAGA
  CAACTGAAAAGTTGGATGCAGAACCTAAACCCTGCTCAGATACAAACAGTGACTCCCCAGATGAAGGTCT
  AGATCACAATATTCACATTAAATGTAAACGAGAACATCAAGGTTATTCCTCAGAATCCTCCATTTGTGCT
  TCTAAAAGGCCCTGTACAGAGGATACCATGTTGGAACTTCTGTTACGCTTGAAACATTTAAGCTTGAAAA
  ACTCAATAACACATGGATCTTTCTCAGGGTCATTGCAGGGGTACCCATCCAGTGGTGCTAAGTCTCTTCA
  GTCAGTTTCATCTATCTCAGACCTTAATTTTCAGAATCAAGATGAAAACATGCCAAGTCAGTACCTTGCA
  CAGTTGGCGGCTAAGCCGTTTTTCTGTGAGCTTCAAGGATGCAAATATGAATTTGTGACCAGAGAGGCTC
  TGTTAATGCATTATCTTAAAAAGCATAATTATTCAAAAGAAAAAGTCCTTCAGTTAACCATGTTCCAACA
  TCGGTATTCCCCATTTCAGTGTCATATTTGCCAAAGGTCATTTACAAGAAAAACACACCTTAGGATTCAT
  TATAAAAATAAACATCAAATTGGCAGTGACAGAGCAACTCACAAACTATTAGATAATGAAAAGTGTGATC
  ATGAAGGCCCATGTTCAGTAGATAGGTTGAAAGGTGATTGTTCTGCAGAACTTGGAGGTGATCCCAGTAG
  TAACTCTGAGAAACCACACTGTCATCCTAAAAAGGATGAATGTAGTTCTGAAACAGATTTGGAATCATCT
  TGTGAAGAAACAGAAAGTAAAACATCTGACATTTCATCACCAATAGGCAGCCATAGAGAAGAACAAGAAG
  GAAGAGAGGGCAGAGGTAGCAGGCGAACTGTTGCTAAAGGAAATCTGTGTTATATTTTGAATAAATACCA
  CAAACCATTCCATTGTATTCATAAAACTTGCAACTCCTCATTCACCAATCTAAAAGGCTTAATTCGCCAT
  TACAGAACTGTACATCAGTACAACAAAGAACAGTTATGTTTGGAGAAAGACAAAGCAAGAACCAAAAGGG
  AACTTGTCAAATGTAAAAAGATATTTGCTTGCAAATATAAGGAATGTAATAAACGCTTCCTGTGTTCCAA
  AGCTCTTGCTAAGCACTGTAGTGATTCTCATAACCTAGACCATATTGAAGAGCCTAAAGTACTTTCCGAA
  GCTGGATCTGCAGCAAGGTTTTCTTGTAACCAGCCTCAGTGCCCTGCTGTTTTTTATACATTCAACAAGT
  TGAAGCACCACTTGATGGAACAGCATAATATTGAAGGGGAAATACATTCAGATTATGAAATTCATTGTGA
  TCTTAATGGCTGTGGCCAGATTTTCACCCATCGCAGTAATTACTCACAACATGTATATTACCGACATAAA
  GACTATTATGATGATTTGTTTAGAAGCCAGAAAGTAGCAAATGAGAGACTACTAAGGAGTGAAAAGGTAT
  GTCAAACAGCTGATACTCAGGGGCATGAACATCAGACCACCAGGAGATCATTTAATGCTAAGTCTAAAAA
  ATGTGGCTTAATCAAAGAAAAGAAAGCCCCAATAAGTTTTAAAACCAGAGCTGAGGCCCTCCATATGTGT
  GTGGAGCACTCTGAGCACACACAGTACCCCTGCATGGTTCAAGGATGCTTATCTGTGGTGAAGTTGGAGA
  GCAGCATTGTGAGGCATTACAAACGCACTCATCAGATGAGTAGTGCCTATTTAGAGCAACAGATGGAGAA
  TCTTGTTGTTTGCGTTAAGTACGGTACCAAAATTAAGGAGGAACCCCCTTCTGAAGCAGATCCCTGTATA
  AAGAAAGAAGAAAATAGAAGCTGTGAATCAGAGCGCACAGAACACAGCCATTCCCCGGGTGACAGTAGTG
  CACCCATCCAGAACACTGATTGCTGTCATTCAAGTGAAAGGGATGGAGGTCAGAAAGGGTGCATAGAAAG
  CAGCTCAGTATTTGATGCAGATACTCTGCTCTACAGGGGAACTTTGAAATGTAATCATAGTTCCAAAACC
  ACTTCCCTAGAACAGTGTAATATAGTTCAGCCTCCTCCTCCTTGTAAAATAGAAAATTCCATACCTAATC
  CCAATGGGACTGAAAGTGGGACTTATTTCACAAGTTTCCAGCTGCCTTTACCAAGGATCAAAGAATCAGA
  AACTAGGCAGCATAGTTCAGGGCAAGAAAACACTGTAAAAAATCCAACCCATGTCCCAAAAGAGAATTTT
  AGGAAACATTCACAGCCCCGGTCATTTGATTTGAAGACTTACAAACCTATGGGATTTGAATCTTCATTTC
  TGAAATTTATTCAGGAAAGTGAAGAGAAAGAAGATGATTTTGATGATTGGGAGCCTTCAGAGCACTTAAC
  ATTAAGTAATTCTTCACAGTCCAGTAATGATTTAACAGGGAATGTTGTGGCAAATAATATGGTGAATGAC
  AGTGAACCTGAAGTTGACATACCTCATTCTTCCAGTGACTCTACAATTCATGAGAACCTGACTGCAATCC
  CACCTTTAATAGTAGCTGAAACAACAACAGTTCCTTCCTTGGAAAACCTGAGGGTTGTATTGGACAAAGC
  ATTAACAGACTGTGGAGAGCTTGCCTTAAAACAGCTTCATTATCTTCGGCCAGTGGTGGTTCTTGAAAGA
  TCTAAGTTTTCCACACCAATTTTAGACTTATTTCCAACAAAAAAGACAGATGAGCTTTGTGTAGGAAGTT
  CA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC223079 representing NM_012421
  Red=Cloning site Green=Tags(s)
  
  MADGKGDAAAVAGAGAEAPAVAGAGDGVETESMVRGHRPVSPAPGASGLRPCLWQLETELREQEVSEVSS
  LNYCRSFCQTLLQYASNKNASEHIVYLLEVYRLAIQSFASARPYLTTECEDVLLVLGRLVLSCFELLLSV
  SESELPCEVWLPFLQSLQESHDALLEFGNNNLQILVHVTKEGVWKNPVLLKILSQQPVETEEVNKLIAQE
  GPSFLQMRIKHLLKSNCIPQATALSKLCAESKEISNVSSFQQAYITCLCSMLPNEDAIKEIAKVDCKEVL
  DIICNLESEGQDNTAFVLCTTYLTQQLQTASVYCSWELTLFWSKLQRRIDPSLDTFLERCRQFGVIAKTQ
  QHLFCLIRVIQTEAQDAGLGVSILLCVRALQLRSSEDEEMKASVCKTIACLLPEDLEVRRACQLTEFLIE
  PSLDGFNMLEELYLQPDQKFDEENAPVPNSLRCELLLALKAHWPFDPEFWDWKTLKRHCHQLLGQEASDS
  DDDLSGYEMSINDTDVLESFLSDYDEGKEDKQYRRRDLTDQHKEKRDKKPIGSSERYQRWLQYKFFCLLC
  KRECIEARILHHSKMHMEDGIYTCPVCIKKFKRKEMFVPHVMEHVKMPPSRRDRSKKKLLLKGSQKGICP
  KSPSAIPEQNHSLNDQAKGESHEYVTFSKLEDCHLQDRDLYPCPGTDCSRVFKQFKYLSVHLKAEHQNND
  ENAKHYLDMKNRREKCTYCRRHFMSAFHLREHEQVHCGPQPYMCVSIDCYARFGSVNELLNHKQKHDDLR
  YKCELNGCNIVFSDLGQLYHHEAQHFRDASYTCNFLGCKKFYYSKIEYQNHLSMHNVENSNGDIKKSVKL
  EESATGEKQDCINQPHLLNQTDKSHLPEDLFCAESANSQIDTETAENLKENSDSNSSDQLSHSSSASMNE
  ELIDTLDHSETMQDVLLSNEKVFGPSSLKEKCSSMAVCFDGTKFTCGFDGCGSTYKNARGMQKHLRKVHP
  YHFKPKKIKTKDLFPSLGNEHNQTTEKLDAEPKPCSDTNSDSPDEGLDHNIHIKCKREHQGYSSESSICA
  SKRPCTEDTMLELLLRLKHLSLKNSITHGSFSGSLQGYPSSGAKSLQSVSSISDLNFQNQDENMPSQYLA
  QLAAKPFFCELQGCKYEFVTREALLMHYLKKHNYSKEKVLQLTMFQHRYSPFQCHICQRSFTRKTHLRIH
  YKNKHQIGSDRATHKLLDNEKCDHEGPCSVDRLKGDCSAELGGDPSSNSEKPHCHPKKDECSSETDLESS
  CEETESKTSDISSPIGSHREEQEGREGRGSRRTVAKGNLCYILNKYHKPFHCIHKTCNSSFTNLKGLIRH
  YRTVHQYNKEQLCLEKDKARTKRELVKCKKIFACKYKECNKRFLCSKALAKHCSDSHNLDHIEEPKVLSE
  AGSAARFSCNQPQCPAVFYTFNKLKHHLMEQHNIEGEIHSDYEIHCDLNGCGQIFTHRSNYSQHVYYRHK
  DYYDDLFRSQKVANERLLRSEKVCQTADTQGHEHQTTRRSFNAKSKKCGLIKEKKAPISFKTRAEALHMC
  VEHSEHTQYPCMVQGCLSVVKLESSIVRHYKRTHQMSSAYLEQQMENLVVCVKYGTKIKEEPPSEADPCI
  KKEENRSCESERTEHSHSPGDSSAPIQNTDCCHSSERDGGQKGCIESSSVFDADTLLYRGTLKCNHSSKT
  TSLEQCNIVQPPPPCKIENSIPNPNGTESGTYFTSFQLPLPRIKESETRQHSSGQENTVKNPTHVPKENF
  RKHSQPRSFDLKTYKPMGFESSFLKFIQESEEKEDDFDDWEPSEHLTLSNSSQSSNDLTGNVVANNMVND
  SEPEVDIPHSSSDSTIHENLTAIPPLIVAETTTVPSLENLRVVLDKALTDCGELALKQLHYLRPVVVLER
  SKFSTPILDLFPTKKTDELCVGSS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012421
• ORF Size: 5742 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012421.4
• RefSeq Size: 6229 bp
• RefSeq ORF: 5745 bp
• Locus ID: 6018
• UniProt ID: Q13129
• Cytogenetics: 1p34.2
• Domains: zf-C2H2
• MW: 217.8 kDa
• Gene Summary: May be involved in transcriptional regulation.[UniProtKB/Swiss-Prot Function]