PPAPDC1B (PLPP5) (NM_001102560) Human Tagged ORF Clone
CAT#: RC222117
PPAPDC1B (Myc-DDK-tagged)-Human phosphatidic acid phosphatase type 2 domain containing 1B (PPAPDC1B), transcript variant 3
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_001102560" in other vectors (4)
USD 198.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | PPAPDC1B |
Synonyms | DPPL1; HTPAP; PPAPDC1B |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC222117 representing NM_001102560
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGAAGGCGGCGGCGGCGGTGGCCTTTGGGGCCGAAGTGGGCGTGCGGCTCGCGCTGTTCGCGGCCT TCCTGGTGACGGAGCTGCTCCCCCCGTTCCAGAGACTCATCCAGCCGGAGGAGATGTGGCTCTACCGGAA CCCCTACGTGGAGGCGGAGTATTTCCCCACCAAGCCGATGTTTGTTATTGCATTTCTCTCTCCACTGTCT CTGATCTTCCTGGCCAAATTTCTCAAGAAGGCAGACACAAGAGACAGCAGACAAGCCTGCCTGGCTGCCA GCCTTGCCCTGGCTCTGAATGGCGTCTTTACCAACACAATAAAACTGATCGTAGGGAGGCCACGCCCAGA TTTCTTCTACCGCTGCTTCCCTGATGGGCTAGCCCATTCTGACTTGATGTGTACAGGGGATAAGGACGTG GTGAATGAGGGCCGAAAGAGCTTCCCCAGTGGACATTCTTCCTGTATGAATATCATATGGACTCTACCCT ATGCTTGTTTTCACATCATTGAATTTAACCTAGAAAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC222117 representing NM_001102560
Red=Cloning site Green=Tags(s) MGKAAAAVAFGAEVGVRLALFAAFLVTELLPPFQRLIQPEEMWLYRNPYVEAEYFPTKPMFVIAFLSPLS LIFLAKFLKKADTRDSRQACLAASLALALNGVFTNTIKLIVGRPRPDFFYRCFPDGLAHSDLMCTGDKDV VNEGRKSFPSGHSSCMNIIWTLPYACFHIIEFNLES TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001102560 |
ORF Size | 528 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001102560.2 |
RefSeq Size | 776 bp |
RefSeq ORF | 531 bp |
Locus ID | 84513 |
UniProt ID | Q8NEB5 |
Cytogenetics | 8p11.23 |
Protein Families | Transmembrane |
MW | 19.5 kDa |
Gene Summary | Displays magnesium-independent phosphatidate phosphatase activity in vitro. Catalyzes the conversion of phosphatidic acid to diacylglycerol. May be a metastatic suppressor for hepatocellular carcinoma.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC222117L3 | Lenti ORF clone of Human phosphatidic acid phosphatase type 2 domain containing 1B (PPAPDC1B), transcript variant 3, Myc-DDK-tagged |
USD 600.00 |
|
RC222117L4 | Lenti ORF clone of Human phosphatidic acid phosphatase type 2 domain containing 1B (PPAPDC1B), transcript variant 3, mGFP tagged |
USD 600.00 |
|
RG222117 | PPAPDC1B (tGFP-tagged) - Human phosphatidic acid phosphatase type 2 domain containing 1B (PPAPDC1B), transcript variant 3 |
USD 500.00 |
|
SC316902 | PPAPDC1B (untagged)-Human phosphatidic acid phosphatase type 2 domain containing 1B (PPAPDC1B), transcript variant 3 |
USD 330.00 |
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