SMC1L2 (SMC1B) (NM_148674) Human Tagged ORF Clone
CAT#: RC221101
SMC1B (Myc-DDK-tagged)-Human structural maintenance of chromosomes 1B (SMC1B)
ORF Plasmid: tGFP
Lentiviral Particles: DDK DDK w/ Puro mGFP mGFP w/ Puro
"NM_148674" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | SMC1L2 |
Synonyms | SMC1BETA; SMC1L2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC221101 representing NM_148674
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCACCTGGAGCTGCTGCTTGTGGAAAATTTCAAGTCGTGGCGGGGCCGCCAGGTCATTGGCCCCT TCCGGAGGTTCACCTGCATCATCGGCCCCAACGGCTCTGGAAAATCTAATGTAATGGATGCACTTAGTTT TGTAATGGGAGAGAAAATAGCTAATTTAAGAGTGAAAAATATTCAAGAACTCATTCATGGAGCACATATT GGAAAACCTATTTCTTCTTCTGCAAGTGTAAAAATTATATATGTGGAGGAAAGTGGCGAAGAGAAAACAT TTGCAAGGATTATCCGAGGGGGATGCTCAGAATTTCGCTTTAATGATAATCTTGTGAGTCGTTCTGTTTA CATTGCAGAGTTGGAAAAGATAGGCATAATAGTCAAAGCACAAAATTGTTTGGTTTTTCAGGGAACTGTA GAGTCAATTTCAGTGAAGAAACCCAAAGAAAGGACCCAGTTTTTTGAGGAAATCAGCACTTCAGGAGAGC TTATAGGAGAATATGAAGAAAAGAAAAGAAAGTTACAAAAAGCCGAAGAGGATGCACAGTTTAACTTTAA TAAGAAAAAAAATATAGCGGCAGAGCGCAGACAAGCAAAATTAGAGAAGGAAGAGGCAGAACGTTACCAG AGTCTCCTTGAAGAACTGAAAATGAACAAGATACAACTGCAGCTTTTTCAACTATACCATAATGAGAAAA AGATTCATCTCCTGAACACCAAGTTAGAGCATGTGAATAGGGATTTGAGTGTCAAAAGAGAGTCTTTGTC TCATCATGAAAACATAGTTAAAGCCAGGAAAAAGGAACATGGAATGCTAACTAGACAACTACAACAAACA GAAAAAGAATTAAAATCGGTTGAAACCCTTTTAAATCAGAAGAGGCCTCAGTACATTAAAGCCAAAGAAA ACACTTCTCACCACCTTAAGAAATTAGATGTGGCTAAGAAATCAATAAAGGACAGCGAAAAACAATGTTC TAAACAGGAAGATGATATAAAAGCCCTGGAGACAGAGCTGGCTGATTTAGATGCTGCATGGAGAAGTTTT GAAAAGCAGATTGAGGAAGAAATTTTACATAAAAAGCGAGACATTGAACTGGAAGCCAGTCAGCTGGATC GTTATAAAGAACTTAAGGAACAAGTAAGAAAGAAAGTAGCTACAATGACTCAACAACTGGAAAAACTGCA GTGGGAACAGAAGACAGATGAAGAAAGACTGGCATTTGAAAAGAGGAGGCATGGAGAAGTTCAGGGAAAT CTAAAACAAATAAAAGAACAAATAGAAGATCATAAAAAACGAATAGAGAAGTTAGAGGAGTATACAAAGA CATGCATGGATTGCTTGAAAGAGAAAAAACAGCAAGAGGAAACCCTAGTGGATGAAATTGAAAAAACAAA ATCAAGAATGTCTGAATTTAATGAAGAATTGAATCTTATTAGAAGTGAATTGCAGAATGCTGGGATTGAT ACCCATGAGGGAAAACGTCAGCAAAAGAGAGCAGAGGTTCTGGAACACCTTAAAAGACTGTACCCAGATT CTGTGTTTGGAAGACTATTTGACCTGTGTCATCCTATTCATAAGAAATACCAGCTGGCTGTTACTAAGGT TTTTGGCCGGTTCATCACTGCCATTGTTGTAGCCTCTGAAAAGGTAGCAAAAGATTGTATTCGATTTCTG AAGGAGGAAAGAGCTGAACCTGAGACATTCCTCGCTCTAGATTACCTTGATATCAAGCCAATCAATGAAA GACTAAGGGAGCTTAAAGGCTGTAAAATGGTGATTGATGTCATAAAGACTCAGTTTCCTCAGCTGAAGAA AGTGATTCAGTTTGTGTGTGGAAATGGTCTTGTTTGTGAGACTATGGAAGAAGCAAGGCATATTGCACTC AGTGGACCTGAAAGACAGAAAACAGTAGCTCTTGATGGAACATTATTTTTAAAATCTGGAGTGATCTCTG GAGGGTCAAGTGACTTAAAATACAAGGCTAGATGCTGGGATGAGAAAGAGTTAAAGAATCTAAGAGACAG ACGAAGCCAGAAAATCCAAGAGCTAAAGGGTTTAATGAAGACACTCCGCAAAGAAACAGATTTGAAACAA ATACAGACCCTGATACAGGGAACTCAAACACGACTCAAATATTCACAAAATGAACTAGAGATGATTAAGA AGAAGCACCTTGTTGCTTTTTACCAGGAACAATCTCAGTTACAAAGTGAACTACTAAATATTGAGTCTCA ATGTATTATGTTGAGTGAAGGAATCAAGGAACGACAACGAAGAATTAAAGAATTTCAAGAAAAGATAGAT AAGGTAGAAGACGATATCTTCCAACACTTCTGTGAAGAAATTGGCGTGGAAAATATTCGTGAATTTGAGA ACAAACATGTTAAACGGCAACAAGAAATTGATCAAAAAAGATTAGAATTTGAAAAACAAAAAACTCGGCT TAATGTTCAACTTGAGTATAGTCGCAGTCACCTTAAGAAGAAACTGAATAAGATCAACACATTAAAAGAA ACTATCCAGAAAGGTAGTGAAGATATTGATCACCTAAAGAAGGCTGAAGAAAACTGTCTGCAGACAGTGA ATGAACTCATGGCAAAGCAGCAGCAACTTAAGGACATACGTGTCACTCAGAACTCCAGTGCCGAGAAAGT TCAAACTCAAATTGAAGAGGAACGGAAGAAGTTTCTGGCTGTTGATAGGGAAGTGGGGAAATTGCAAAAA GAAGTTGTAAGTATTCAAACTTCTCTGGAACAGAAACGATTAGAGAAGCATAACTTGCTGCTTGATTGCA AAGTGCAAGACATTGAGATAATCCTTTTGTCGGGGTCACTGGATGACATCATTGAAGTGGAGATGGGAAC TGAAGCAGAAAGTACCCAGGCAACAATTGATATCTATGAAAAAGAAGAAGCCTTTGAAATAGACTACAGC TCTCTAAAAGAGGATTTGAAGGCTCTACAGTCTGATCAAGAAATCGAGGCCCACCTTAGGCTCTTATTGC AGCAAGTAGCATCCCAGGAAGATATCTTACTGAAAACAGCAGCCCCAAACCTACGAGCACTGGAGAACTT AAAGACTGTCAGAGACAAGTTTCAAGAGTCCACAGATGCTTTTGAGGCCAGCAGAAAGGAAGCCAGACTG TGTAGGCAAGAGTTCGAGCAAGTGAAAAAAAGGAGATACGATCTTTTCACCCAGTGTTTTGAGCATGTCT CAATCTCAATTGATCAAATCTACAAGAAGCTCTGCAGAAACAACAGCGCCCAAGCATTTCTTAGCCCAGA GAACCCTGAAGAACCTTACTTGGAGGGAATTAGCTATAACTGTGTGGCCCCAGGCAAACGGTTTATGCCA ATGGACAATTTGTCAGGGGGAGAAAAGTGTGTGGCAGCCTTGGCTCTCCTGTTTGCTGTGCACAGTTTTC GTCCTGCCCCATTCTTTGTTTTAGATGAAGTGGATGCAGCCCTAGACAATACTAACATAGGCAAAGTGTC AAGTTACATCAAAGAGCAAACTCAAGACCAGTTTCAGATGATAGTCATCTCCCTAAAAGAAGAGTTCTAT TCCAGAGCCGACGCGCTGATCGGCATCTATCCTGAGTACGATGACTGCATGTTCAGCCGAGTTTTGACCC TAGATCTTTCTCAGTATCCAGACACTGAAGGCCAAGAAAGCAGCAAGAGACACGGAGAGTCCCGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC221101 representing NM_148674
Red=Cloning site Green=Tags(s) MAHLELLLVENFKSWRGRQVIGPFRRFTCIIGPNGSGKSNVMDALSFVMGEKIANLRVKNIQELIHGAHI GKPISSSASVKIIYVEESGEEKTFARIIRGGCSEFRFNDNLVSRSVYIAELEKIGIIVKAQNCLVFQGTV ESISVKKPKERTQFFEEISTSGELIGEYEEKKRKLQKAEEDAQFNFNKKKNIAAERRQAKLEKEEAERYQ SLLEELKMNKIQLQLFQLYHNEKKIHLLNTKLEHVNRDLSVKRESLSHHENIVKARKKEHGMLTRQLQQT EKELKSVETLLNQKRPQYIKAKENTSHHLKKLDVAKKSIKDSEKQCSKQEDDIKALETELADLDAAWRSF EKQIEEEILHKKRDIELEASQLDRYKELKEQVRKKVATMTQQLEKLQWEQKTDEERLAFEKRRHGEVQGN LKQIKEQIEDHKKRIEKLEEYTKTCMDCLKEKKQQEETLVDEIEKTKSRMSEFNEELNLIRSELQNAGID THEGKRQQKRAEVLEHLKRLYPDSVFGRLFDLCHPIHKKYQLAVTKVFGRFITAIVVASEKVAKDCIRFL KEERAEPETFLALDYLDIKPINERLRELKGCKMVIDVIKTQFPQLKKVIQFVCGNGLVCETMEEARHIAL SGPERQKTVALDGTLFLKSGVISGGSSDLKYKARCWDEKELKNLRDRRSQKIQELKGLMKTLRKETDLKQ IQTLIQGTQTRLKYSQNELEMIKKKHLVAFYQEQSQLQSELLNIESQCIMLSEGIKERQRRIKEFQEKID KVEDDIFQHFCEEIGVENIREFENKHVKRQQEIDQKRLEFEKQKTRLNVQLEYSRSHLKKKLNKINTLKE TIQKGSEDIDHLKKAEENCLQTVNELMAKQQQLKDIRVTQNSSAEKVQTQIEEERKKFLAVDREVGKLQK EVVSIQTSLEQKRLEKHNLLLDCKVQDIEIILLSGSLDDIIEVEMGTEAESTQATIDIYEKEEAFEIDYS SLKEDLKALQSDQEIEAHLRLLLQQVASQEDILLKTAAPNLRALENLKTVRDKFQESTDAFEASRKEARL CRQEFEQVKKRRYDLFTQCFEHVSISIDQIYKKLCRNNSAQAFLSPENPEEPYLEGISYNCVAPGKRFMP MDNLSGGEKCVAALALLFAVHSFRPAPFFVLDEVDAALDNTNIGKVSSYIKEQTQDQFQMIVISLKEEFY SRADALIGIYPEYDDCMFSRVLTLDLSQYPDTEGQESSKRHGESR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_148674 |
ORF Size | 3705 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_148674.3, NP_683515.3 |
RefSeq Size | 4266 bp |
RefSeq ORF | 3708 bp |
Locus ID | 27127 |
UniProt ID | Q8NDV3 |
Cytogenetics | 22q13.31 |
Domains | SMC_N, SMC_C |
Protein Pathways | Cell cycle, Oocyte meiosis |
MW | 143.7 kDa |
Gene Summary | SMC1L2 belongs to a family of proteins required for chromatid cohesion and DNA recombination during meiosis and mitosis (3:Revenkova et al., 2001 [PubMed 11564881]).[supplied by OMIM, Mar 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC221101L1 | Lenti ORF clone of Human structural maintenance of chromosomes 1B (SMC1B), Myc-DDK-tagged |
USD 1,375.00 |
|
RC221101L2 | Lenti ORF clone of Human structural maintenance of chromosomes 1B (SMC1B), mGFP tagged |
USD 1,375.00 |
|
RC221101L3 | Lenti ORF clone of Human structural maintenance of chromosomes 1B (SMC1B), Myc-DDK-tagged |
USD 1,375.00 |
|
RC221101L4 | Lenti ORF clone of Human structural maintenance of chromosomes 1B (SMC1B), mGFP tagged |
USD 1,375.00 |
|
RG221101 | SMC1B (tGFP-tagged) - Human structural maintenance of chromosomes 1B (SMC1B) |
USD 1,275.00 |
|
SC315290 | SMC1B (untagged)-Human structural maintenance of chromosomes 1B (SMC1B) |
USD 1,183.00 |
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