ZNF673 (KRBOX4) (NM_017776) Human Tagged ORF Clone

CAT#: RC220264

KRBOX4 (Myc-DDK-tagged)-Human zinc finger family member 673 (ZNF673), transcript variant 2

Product Images

Circular map for RC220264

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  Western blot validation of overexpression lysate (Cat# LY413549) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC220264 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZNF673
• Synonyms: ZNF673
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC220264 representing NM_017776
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCATGTCCCAGGAATCATTGACCTTCAAGGACGTGTTTGTGGACTTCACCCTGGAGGAGTGGCAGC
  AACTGGACTCTGCCCAGAAGAACCTCTACAGGGATGTCATGCTTGAGAACTACAGCCACCTGGTGTCCGT
  GGGGTATCTAGTTGCGAAGCCAGATGTGATCTTCAGGTTGGGACCAGGTGAAGAGTCCTGGATGGCAGAT
  GGGGGGACCCCGGTACGGACCTGTGCAGAAGTCTGGCAAGTTGATGAGCAGATAGATCACTACAAGGAAA
  GCCAAGACAAACTTCCTTGGCAAGCTGCATTCATAGGCAAGGAAACACTGAAGGATGAAAGCGGTCAAGA
  ATCCAGAACATGTAGAAAAAGCATTTATCTGAGCACAGAATTTGATTCTGTAAGGCAAAGACTCCCTAAA
  TATTATTCGTGGGAAAAGGCATTCAAAACATCATTTAAACTTTCTTGGTCAAAATGGAAGCTATGTAAGA
  AAGAAAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC220264 representing NM_017776
  Red=Cloning site Green=Tags(s)
  
  MAMSQESLTFKDVFVDFTLEEWQQLDSAQKNLYRDVMLENYSHLVSVGYLVAKPDVIFRLGPGEESWMAD
  GGTPVRTCAEVWQVDEQIDHYKESQDKLPWQAAFIGKETLKDESGQESRTCRKSIYLSTEFDSVRQRLPK
  YYSWEKAFKTSFKLSWSKWKLCKKER
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017776
• ORF Size: 498 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017776.2
• RefSeq Size: 2308 bp
• RefSeq ORF: 501 bp
• Locus ID: 55634
• UniProt ID: Q5JUW0
• Cytogenetics: Xp11.3
• Domains: KRAB
• Protein Families: Transcription Factors
• MW: 19.4 kDa
• Gene Summary: This encodes a zinc finger protein with an N-terminal KRAB (Kruppel-associated) domain found in transcriptional repressors. This gene is located in a region of the X chromosome thought to be involved in nonsyndromic X-linked cognitive disability. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]