ADCK2 (NM_052853) Human Tagged ORF Clone

CAT#: RC220159

ADCK2 (Myc-DDK-tagged)-Human aarF domain containing kinase 2 (ADCK2)

Product Images

Circular map for RC220159

• 

  Western blot validation of overexpression lysate (Cat# LY403259) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC220159 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ADCK2
• Synonyms: AARF
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC220159 representing NM_052853
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGGCGCCCTGGCGCGTCTCCGTCAGGGTTTGCCTGTCGCACCTGAGGTGCTTCGAGCTCAGACAGG
  GACTCAGCCTCCTGAGGCCCTCCGAGTGCCCTCGCGATGCCAGGCTCTGCTGGCTTCTGCTGGGCACTTT
  GCCCAAGGTCGTCTCCCTGTGCGGGGACGTGGGTGAGGGGGCCCCTGACGTTCTGAGTCGGCGAAGGGTC
  CGCTGCAGCGGGGCGGCTGGCGCGGGGCCCGCGGAGAGCCTCCCCCGAGCGGGACCTCTGGGCGGCGTCT
  TCCTGCATCTCCGCCTCTGGCTTCGCGCCGGCGCTCTGTTGGTGAAATTCTTCCCCCTCCTACTCCTCTA
  CCCCCTCACCTACCTGGCTCCCAGCGTCTCCACCCTCTGGCTCCACCTGCTTCTGAAAGCCACCGAGACC
  TCAGGCCCAACCTACATCAAACTGGGCCAGTGGGCCAGCACCCGGCGCGATCTGTTTTCGGAGGCTTTCT
  GTGCCCAATTTTCCAAGCTGCATGTCCGAGTGACGCCCCACCCGTGGACTCACACTGAGCGCTTCCTTCG
  GCAGGCTTTTGGGGATGACTGGGGGAGCATCCTCTCTTTTGAGAACCGGGAACCTGTGGGCTCAGGCTGC
  GTGGCCCAGGTGTACAAAGCATACGCCAACACTGCCTTCCTGGAGACTGACAGCGTCCAGAGACTTGGCA
  GGGCCTCCTGTCTGCCGCCCTTCTCACATACTGGGGCAGTCGGTGGGCTGAGAGAGCTCTTTGGATACCT
  TGGAAATGGCCGGAAACCTCCAGAAAATCTCGCAGACCAGTCGTTTCTAGAAAGGCTGCTCCTCCCTAAA
  GCTGACCTGGTTGGATCAAATGCAGGGGTGTCTCGGGCTCAGGTCCCTGGCCACCAACCTGAGGCCACCA
  ACCTCATCTCCGTGGCAGTGAAAGTGTTGCACCCTGGCCTGCTCGCTCAGGTGCATATGGACCTGCTGCT
  GATGAAGATTGGCAGCCGAGTCCTGGGAGTTTTGCCAGGCATCAAGTGGCTTAGCTTGCCTGAGATTGTG
  GAGGAATTTGAGAAGCTGATGGTCCAACAGATTGACCTGCGTTACGAAGCTCAGAATCTAGAACACTTCC
  AGGTCAACTTCCGGAATGTGAAAGCCGTCAAGTTCCCCACCCCTCTGCGCCCCTTTGTCACCAGAGAAGT
  CTTGGTGGAAACGTATGAAGAGAGTGTGCCTGTGTCCAGTTACCAGCAGGCAGGAATTCCCGTGGACTTG
  AAAAGGAAGATTGCACGGCTGGGGATCAACATGCTCCTGAAGATGATATTTGTGGATAACTTTGTCCATG
  CAGACCTTCACCCTGGAAACATCCTGGTTCAGGGTGCCAACGGCCTGTCCTCGAGTCAGGAGGCGCAGCT
  GCAGCAGGCGGACATCTGTGACACTCTGGTGGTGGCCGTGCCATCTTCCCTCTGCCCGCTGCGACTGGTG
  CTGCTGGATGCTGGCATTGTGGCGGAGCTGCAGGCCCCTGACCTGAGGAATTTCCGGGCAGTTTTCATGG
  CTGTGGTGATGGGGCAGGGCCAGAGAGTGGCTGAGCTGATCCTGCATCATGCCCGGGCCAGCGAGTGCAG
  GGACGTGGAGGGGTTCAAAACCGAGATGGCCATGCTGGTGACCCAGGCCAGGAAGAACACCATCACCCTG
  GAGAAGCTTCATGTGTCCAGCCTTCTCTCTAGTGTCTTTAAGTTGCTGATGACTCACAAGGTAAAGCTTG
  AGAGCAACTTTGCCTCCATTGTGTTTGCCATCATGGTGTTGGAGGGGCTTGGCCGCTCACTGGACCCCAA
  ACTGGACATCCTGGAGGCAGCGAGGCCCTTCCTCCTCACGGGCCCAGTGTGCCCCCCG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC220159 representing NM_052853
  Red=Cloning site Green=Tags(s)
  
  MVAPWRVSVRVCLSHLRCFELRQGLSLLRPSECPRDARLCWLLLGTLPKVVSLCGDVGEGAPDVLSRRRV
  RCSGAAGAGPAESLPRAGPLGGVFLHLRLWLRAGALLVKFFPLLLLYPLTYLAPSVSTLWLHLLLKATET
  SGPTYIKLGQWASTRRDLFSEAFCAQFSKLHVRVTPHPWTHTERFLRQAFGDDWGSILSFENREPVGSGC
  VAQVYKAYANTAFLETDSVQRLGRASCLPPFSHTGAVGGLRELFGYLGNGRKPPENLADQSFLERLLLPK
  ADLVGSNAGVSRAQVPGHQPEATNLISVAVKVLHPGLLAQVHMDLLLMKIGSRVLGVLPGIKWLSLPEIV
  EEFEKLMVQQIDLRYEAQNLEHFQVNFRNVKAVKFPTPLRPFVTREVLVETYEESVPVSSYQQAGIPVDL
  KRKIARLGINMLLKMIFVDNFVHADLHPGNILVQGANGLSSSQEAQLQQADICDTLVVAVPSSLCPLRLV
  LLDAGIVAELQAPDLRNFRAVFMAVVMGQGQRVAELILHHARASECRDVEGFKTEMAMLVTQARKNTITL
  EKLHVSSLLSSVFKLLMTHKVKLESNFASIVFAIMVLEGLGRSLDPKLDILEAARPFLLTGPVCPP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_052853
• ORF Size: 1878 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_052853.4
• RefSeq Size: 2376 bp
• RefSeq ORF: 1881 bp
• Locus ID: 90956
• UniProt ID: Q7Z695
• Cytogenetics: 7q34
• Domains: ABC1
• Protein Families: Druggable Genome, Protein Kinase
• MW: 68.8 kDa
• Gene Summary: The function of this protein is not yet clear. It is not known if it has protein kinase activity and what type of substrate it would phosphorylate (Ser, Thr or Tyr).[UniProtKB/Swiss-Prot Function]