SLC17A4 (NM_005495) Human Tagged ORF Clone

CAT#: RC219277

SLC17A4 (Myc-DDK-tagged)-Human solute carrier family 17 (sodium phosphate), member 4 (SLC17A4)

Product Images

Circular map for RC219277

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SLC17A4
• Synonyms: KAIA2138
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC219277 representing NM_005495
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTACCGGACCAGATGTCAAGGCTACAGTGGGGGACATTTCCAGTGATGGCAATTTAAACGTGGCTC
  AAGAGGAATGCTCCAGGAAAGGTTTTTGTTCAGTCCGACATGGGCTGGCCCTCATCTTGCAGCTCTGTAA
  TTTTTCAATTTACACCCAACAAATGAACTTGAGCATTGCCATTCCAGCTATGGTGAACAACACAGCCCCA
  CCTAGCCAGCCCAATGCCTCCACAGAACGGCCCTCCACTGACTCCCAGGGCTACTGGAATGAAACTCTAA
  AAGAATTTAAAGCAATGGCCCCTGCATATGACTGGAGTCCTGAAATCCAGGGAATCATCCTCAGCTCCCT
  CAACTATGGCTCATTCTTGGCTCCAATCCCCAGTGGCTATGTGGCTGGAATATTTGGAGCCAAGTATGTG
  GTTGGTGCTGGCTTGTTTATTTCCTCATTCCTGACCCTCTTCATTCCACTGGCAGCTAATGCGGGAGTGG
  CCTTGCTCATTGTCCTCCGGATTGTACAAGGCATAGCCCAGGTTATGGTATTAACTGGTCAGTATTCAAT
  TTGGGTCAAATGGGCTCCCCCACTGGAAAGGAGTCAACTCACCACCATTGCTGGATCAGGGTCAATGCTG
  GGGTCCTTCATTGTTCTACTTGCTGGTGGTCTCCTCTGCCAGACCATAGGATGGCCTTACGTCTTCTATA
  TCTTTGGAGGAATTGGCTGTGCTTGTTGTCCTCTCTGGTTTCCTCTCATTTATGATGATCCTGTGAATCA
  TCCCTTTATCAGTGCTGGTGAGAAGAGATACATTGTGTGTTCATTGGCTCAGCAGGACTGTTCACCAGGC
  TGGTCTCTTCCCATTAGGGCTATGATCAAATCCTTACCACTCTGGGCCATTTTAGTCTCTTATTTCTGTG
  AATACTGGCTTTTTTATACCATTATGGCGTACACACCAACGTACATCAGCTCGGTACTTCAAGCCAACCT
  CAGAGATAGTGGGATCCTGTCTGCCTTGCCGTTTGTTGTTGGATGTATCTGCATTATCCTTGGAGGTCTA
  CTGGCAGACTTTCTTCTCTCCAGAAAAATCCTCAGACTCATCACCATCAGGAAACTCTTCACTGCCATTG
  GGGTTCTCTTCCCATCCGTGATCCTCGTGTCCCTGCCCTGGGTCAGATCCAGCCACAGCATGACCATGAC
  CTTCTTGGTGCTGTCTTCTGCCATCAGCAGCTTCTGTGAATCAGGAGCCCTTGTTAACTTCTTGGATATT
  GCTCCTCGGTACACTGGCTTTCTCAAAGGACTATTGCAAGTCTTTGCACACATAGCTGGAGCCATCTCTC
  CTACTGCTGCTGGATTTTTCATCAGTCAGGATTCAGAGTTTGGTTGGAGAAATGTCTTCTTGCTTTCAGC
  TGCTGTTAACATATCGGGCCTGGTTTTCTACCTCATCTTTGGCCGAGCAGATGTGCAGGACTGGGCTAAG
  GAGCAGACATTCACCCACCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC219277 representing NM_005495
  Red=Cloning site Green=Tags(s)
  
  MSTGPDVKATVGDISSDGNLNVAQEECSRKGFCSVRHGLALILQLCNFSIYTQQMNLSIAIPAMVNNTAP
  PSQPNASTERPSTDSQGYWNETLKEFKAMAPAYDWSPEIQGIILSSLNYGSFLAPIPSGYVAGIFGAKYV
  VGAGLFISSFLTLFIPLAANAGVALLIVLRIVQGIAQVMVLTGQYSIWVKWAPPLERSQLTTIAGSGSML
  GSFIVLLAGGLLCQTIGWPYVFYIFGGIGCACCPLWFPLIYDDPVNHPFISAGEKRYIVCSLAQQDCSPG
  WSLPIRAMIKSLPLWAILVSYFCEYWLFYTIMAYTPTYISSVLQANLRDSGILSALPFVVGCICIILGGL
  LADFLLSRKILRLITIRKLFTAIGVLFPSVILVSLPWVRSSHSMTMTFLVLSSAISSFCESGALVNFLDI
  APRYTGFLKGLLQVFAHIAGAISPTAAGFFISQDSEFGWRNVFLLSAAVNISGLVFYLIFGRADVQDWAK
  EQTFTHL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005495
• ORF Size: 1491 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005495.1, NP_005486.1
• RefSeq Size: 2626 bp
• RefSeq ORF: 1494 bp
• Locus ID: 10050
• UniProt ID: Q9Y2C5
• Cytogenetics: 6p22.2
• Protein Families: Transmembrane
• MW: 53.9 kDa
• Gene Summary: Phosphate homeostasis is maintained by regulating intake, intestinal absorption, bone deposition and resorption, and renal excretion of phosphate. The central molecule in the control of phosphate excretion from the kidney is the sodium/phosphate cotransporter NPT1 (SLC17A1; MIM 182308), which is located in the renal proximal tubule. NPT1 uses the transmembrane electrochemical potential gradient of sodium to transport phosphate across the cell membrane. SLC17A4 is a similar sodium/phosphate cotransporter in the intestinal mucosa that plays an important role in the absorption of phosphate from the intestine (summary by Shibui et al., 1999 [PubMed 10319585]).[supplied by OMIM, Feb 2011]