FMNL2 (NM_052905) Human Tagged ORF Clone

CAT#: RC219109

FMNL2 (Myc-DDK-tagged)-Human formin-like 2 (FMNL2)

Product Images

Circular map for RC219109

• 

  Western blot validation of overexpression lysate (Cat# LY409402) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC219109 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified FMNL2 protein (Cat# TP319109). The protein was produced from HEK293T cells transfected with FMNL2 cDNA clone (Cat# RC219109) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FMNL2
• Synonyms: FHOD2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC219109 representing NM_052905
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCAACGCAGGGAGCATGGATTCGCAGCAGACCGATTTCAGGGCGCACAACGTGCCTTTGAAGCTGC
  CGATGCCAGAGCCAGGTGAACTGGAGGAGCGATTTGCCATCGTGCTGAATGCTATGAACCTACCTCCTGA
  CAAAGCCAGGTTACTGCGGCAGTATGATAATGAGAAAAAATGGGAACTGATTTGTGATCAGGAACGATTC
  CAGGTGAAGAATCCTCCCCATACATACATTCAAAAGCTCAAAGGCTATCTGGATCCAGCTGTAACCAGGA
  AGAAATTCAGACGGCGTGTTCAAGAATCTACACAAGTGCTAAGAGAACTGGAAATTTCTTTGAGAACTAA
  CCACATTGGATGGGTCAGAGAATTTCTGAATGAAGAAAACAAAGGTCTTGATGTTCTAGTGGAATATCTC
  TCATTTGCACAGTACGCGGTAACTTTTGACTTTGAAAGTGTGGAGAGTACTGTGGAGAGCTCGGTGGACA
  AATCAAAGCCCTGGAGTAGGTCCATCGAGGACCTGCACAGAGGGAGCAACCTGCCCTCACCTGTGGGCAA
  CAGTGTCTCCCGCTCTGGAAGACATTCTGCACTGCGATATAATACATTGCCAAGCAGAAGAACTCTGAAA
  AATTCAAGATTAGTGAGTAAGAAAGATGATGTGCATGTCTGTATCATGTGTTTACGTGCCATCATGAATT
  ATCAGTATGGTTTCAACATGGTCATGTCTCATCCACACGCTGTCAATGAGATTGCACTAAGCCTGAACAA
  CAAGAATCCCAGAACAAAAGCCCTTGTCTTAGAACTGTTGGCAGCCGTTTGTCTTGTCAGAGGCGGGCAT
  GAAATCATTTTATCAGCATTTGATAACTTTAAAGAGGTTTGTGGAGAAAAACAGCGCTTTGAGAAGTTGA
  TGGAACATTTCAGGAATGAAGACAATAACATAGATTTTATGGTGGCTTCTATGCAGTTTATTAATATTGT
  AGTCCATTCAGTAGAAGATATGAATTTCAGAGTTCACCTGCAGTATGAATTTACCAAATTAGGCCTGGAC
  GAATACTTGGACAAGCTGAAACACACTGAGAGTGACAAGCTTCAAGTCCAGATCCAGGCTTACCTGGACA
  ATGTTTTTGATGTAGGAGCTCTACTGGAAGATGCTGAAACTAAGAATGCTGCCTTGGAGAGGGTGGAAGA
  ACTGGAAGAAAACATTTCTCATTTATCTGAAAAACTGCAAGACACAGAGAATGAAGCCATGTCCAAGATT
  GTGGAACTGGAAAAGCAACTCATGCAGAGGAACAAGGAGCTGGATGTCGTTCGGGAAATCTACAAAGATG
  CAAATACTCAAGTTCACACATTAAGAAAAATGGTCAAAGAAAAAGAAGAAGCAATTCAAAGACAGTCTAC
  CCTGGAAAAAAAGATTCATGAGCTAGAGAAACAAGGGACCATTAAAATTCAGAAGAAAGGGGATGGGGAT
  ATCGCCATACTGCCAGTTGTGGCTTCTGGCACATTGTCCATGGGGTCAGAAGTGGTAGCAGGTAACTCTG
  TGGGACCCACAATGGGGGCCGCTTCCTCAGGACCCTTGCCCCCTCCTCCACCACCACTGCCTCCCTCATC
  AGACACACCTGAAACAGTGCAAAATGGTCCAGTAACACCACCTATGCCACCGCCGCCGCCGCCCCCTCCT
  CCACCTCCTCCTCCCCCACCGCCCCCTCCGCCTCCTCCTCTCCCAGGCCCTGCAGCTGAGACTGTACCAG
  CTCCTCCCTTAGCACCTCCCCTTCCCTCTGCACCTCCGCTGCCTGGAACATCTTCACCCACAGTGGTTTT
  CAACTCAGGATTAGCAGCTGTGAAAATTAAGAAGCCAATCAAGACGAAGTTCAGAATGCCAGTGTTTAAC
  TGGGTTGCTCTGAAGCCCAATCAGATCAATGGCACAGTCTTCAATGAAATTGATGATGAGCGAATTCTGG
  AGGATTTAAATGTGGATGAATTTGAGGAAATATTCAAGACAAAAGCCCAAGGACCTGCCATTGATCTTTC
  TTCAAGCAAACAGAAGATACCACAGAAGGGATCAAACAAAGTGACATTACTAGAAGCAAACAGGGCCAAA
  AATCTTGCCATAACTTTAAGGAAAGCTGGAAAGACTGCTGATGAAATATGTAAAGCTATTCATGTATTTG
  ACTTGAAGACACTGCCTGTGGACTTTGTGGAATGCTTGATGCGGTTCCTACCAACTGAGAATGAAGTGAA
  AGTGCTTCGGCTCTACGAGCGGGAAAGGAAGCCTCTGGAAAACTTGTCAGATGAAGATCGGTTCATGATG
  CAGTTTAGTAAAATCGAGAGGCTCATGCAGAAGATGACCATCATGGCCTTCATTGGGAACTTTGCTGAAA
  GCATTCAGATGCTGACTCCTCAACTACATGCGATTATAGCAGCATCTGTCTCTATAAAGTCGTCCCAAAA
  ACTCAAGAAAATTCTGGAGATCATCTTAGCCCTTGGAAACTACATGAATAGCAGTAAAAGAGGAGCAGTT
  TATGGATTTAAACTTCAGAGTTTAGATCTGCTCTTAGATACAAAGTCAACAGACAGAAAGCAAACACTGT
  TGCACTATATATCCAATGTGGTGAAAGAAAAATATCACCAAGTGTCCCTGTTTTATAATGAGCTTCATTA
  TGTGGAAAAAGCTGCTGCAGTCTCCCTTGAGAATGTTTTGCTGGATGTCAAGGAGCTCCAGAGGGGAATG
  GACTTGACCAAGAGAGAGTACACCATGCATGACCATAACACGCTGCTGAAGGAGTTCATCCTCAACAATG
  AGGGGAAGCTGAAGAAGCTGCAGGATGATGCCAAGATCGCACAGGATGCCTTTGATGATGTTGTGAAGTA
  TTTTGGAGAAAACCCCAAGACAACACCACCCTCTGTCTTCTTTCCTGTCTTTGTCCGGTTTGTGAAAGCA
  TATAAGCAAGCAGAAGAGGAAAATGAGCTGAGGAAAAAGCAGGAACAAGCTCTCATGGAAAAACTCCTAG
  AGCAAGAAGCTCTGATGGAGCAGCAGGATCCAAAGTCTCCTTCTCATAAATCAAAGAGGCAGCAGCAAGA
  GTTAATTGCAGAATTAAGAAGACGACAAGTTAAAGATAACAGACATGTATATGAGGGAAAAGATGGTGCC
  ATTGAAGATATTATCACAGATCTTAGAAACCAACCATACAGACGAGCCGATGCGGTGAGGAGAAGCGTCA
  GGCGGCGCTTTGATGATCAGAACTTGCGTTCTGTTAATGGTGCCGAAATAACAATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC219109 representing NM_052905
  Red=Cloning site Green=Tags(s)
  
  MGNAGSMDSQQTDFRAHNVPLKLPMPEPGELEERFAIVLNAMNLPPDKARLLRQYDNEKKWELICDQERF
  QVKNPPHTYIQKLKGYLDPAVTRKKFRRRVQESTQVLRELEISLRTNHIGWVREFLNEENKGLDVLVEYL
  SFAQYAVTFDFESVESTVESSVDKSKPWSRSIEDLHRGSNLPSPVGNSVSRSGRHSALRYNTLPSRRTLK
  NSRLVSKKDDVHVCIMCLRAIMNYQYGFNMVMSHPHAVNEIALSLNNKNPRTKALVLELLAAVCLVRGGH
  EIILSAFDNFKEVCGEKQRFEKLMEHFRNEDNNIDFMVASMQFINIVVHSVEDMNFRVHLQYEFTKLGLD
  EYLDKLKHTESDKLQVQIQAYLDNVFDVGALLEDAETKNAALERVEELEENISHLSEKLQDTENEAMSKI
  VELEKQLMQRNKELDVVREIYKDANTQVHTLRKMVKEKEEAIQRQSTLEKKIHELEKQGTIKIQKKGDGD
  IAILPVVASGTLSMGSEVVAGNSVGPTMGAASSGPLPPPPPPLPPSSDTPETVQNGPVTPPMPPPPPPPP
  PPPPPPPPPPPPPLPGPAAETVPAPPLAPPLPSAPPLPGTSSPTVVFNSGLAAVKIKKPIKTKFRMPVFN
  WVALKPNQINGTVFNEIDDERILEDLNVDEFEEIFKTKAQGPAIDLSSSKQKIPQKGSNKVTLLEANRAK
  NLAITLRKAGKTADEICKAIHVFDLKTLPVDFVECLMRFLPTENEVKVLRLYERERKPLENLSDEDRFMM
  QFSKIERLMQKMTIMAFIGNFAESIQMLTPQLHAIIAASVSIKSSQKLKKILEIILALGNYMNSSKRGAV
  YGFKLQSLDLLLDTKSTDRKQTLLHYISNVVKEKYHQVSLFYNELHYVEKAAAVSLENVLLDVKELQRGM
  DLTKREYTMHDHNTLLKEFILNNEGKLKKLQDDAKIAQDAFDDVVKYFGENPKTTPPSVFFPVFVRFVKA
  YKQAEEENELRKKQEQALMEKLLEQEALMEQQDPKSPSHKSKRQQQELIAELRRRQVKDNRHVYEGKDGA
  IEDIITDLRNQPYRRADAVRRSVRRRFDDQNLRSVNGAEITM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_052905
• ORF Size: 3276 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_052905.4
• RefSeq Size: 5591 bp
• RefSeq ORF: 3279 bp
• Locus ID: 114793
• UniProt ID: Q96PY5
• Cytogenetics: 2q23.3
• Protein Families: Transcription Factors
• MW: 123.9 kDa
• Gene Summary: This gene encodes a formin-related protein. Formin-related proteins have been implicated in morphogenesis, cytokinesis, and cell polarity. Alternatively spliced transcript variants encoding different isoforms have been described but their full-length nature has yet to be determined. [provided by RefSeq, Jul 2008]