IL31RA (NM_139017) Human Tagged ORF Clone

CAT#: RC218212

IL31RA (Myc-DDK-tagged)-Human interleukin 31 receptor A (IL31RA)

Product Images

Circular map for RC218212

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  Western blot validation of overexpression lysate (Cat# LY408390) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC218212 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified IL31RA protein (Cat# TP318212). The protein was produced from HEK293T cells transfected with IL31RA cDNA clone (Cat# RC218212) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: IL31RA
• Synonyms: CRL; CRL3; GLM-R; GLMR; GPL; hGLM-R; IL-31RA; PLCA2; PRO21384; zcytoR17
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC218212 representing NM_139017
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGCATCAGGCAACTCAAGTTTTTCACCACGGCATGTGTCTGTGAATGTCCGCAAAACATTCTCTCTC
  CCCAGCCTTCATGTGTTAACCTGGGGATGATGTGGACCTGGGCACTGTGGATGCTCCCTTCACTCTGCAA
  ATTCAGCCTGGCAGCTCTGCCAGCTAAGCCTGAGAACATTTCCTGTGTCTACTACTATAGGAAAAATTTA
  ACCTGCACTTGGAGTCCAGGAAAGGAAACCAGTTATACCCAGTACACAGTTAAGAGAACTTACGCTTTTG
  GAGAAAAACATGATAATTGTACAACCAATAGTTCTACAAGTGAAAATCGTGCTTCGTGCTCTTTTTTCCT
  TCCAAGAATAACGATCCCAGATAATTATACCATTGAGGTGGAAGCTGAAAATGGAGATGGTGTAATTAAA
  TCTCATATGACATACTGGAGATTAGAGAACATAGCGAAAACTGAACCACCTAAGATTTTCCGTGTGAAAC
  CAGTTTTGGGCATCAAACGAATGATTCAAATTGAATGGATAAAGCCTGAGTTGGCGCCTGTTTCATCTGA
  TTTAAAATACACACTTCGATTCAGGACAGTCAACAGTACCAGCTGGATGGAAGTCAACTTCGCTAAGAAC
  CGTAAGGATAAAAACCAAACGTACAACCTCACGGGGCTGCAGCCTTTTACAGAATATGTCATAGCTCTGC
  GATGTGCGGTCAAGGAGTCAAAGTTCTGGAGTGACTGGAGCCAAGAAAAAATGGGAATGACTGAGGAAGA
  AGCTCCATGTGGCCTGGAACTGTGGAGAGTCCTGAAACCAGCTGAGGCGGATGGAAGAAGGCCAGTGCGG
  TTGTTATGGAAGAAGGCAAGAGGAGCCCCAGTCCTAGAGAAAACACTTGGCTACAACATATGGTACTATC
  CAGAAAGCAACACTAACCTCACAGAAACAATGAACACTACTAACCAGCAGCTTGAACTGCATCTGGGAGG
  CGAGAGCTTTTGGGTGTCTATGATTTCTTATAATTCTCTTGGGAAGTCTCCAGTGGCCACCCTGAGGATT
  CCAGCTATTCAAGAAAAATCATTTCAGTGCATTGAGGTCATGCAGGCCTGCGTTGCTGAGGACCAGCTAG
  TGGTGAAGTGGCAAAGCTCTGCTCTAGACGTGAACACTTGGATGATTGAATGGTTTCCGGATGTGGACTC
  AGAGCCCACCACCCTTTCCTGGGAATCTGTGTCTCAGGCCACGAACTGGACGATCCAGCAAGATAAATTA
  AAACCTTTCTGGTGCTATAACATCTCTGTGTATCCAATGTTGCATGACAAAGTTGGCGAGCCATATTCCA
  TCCAGGCTTATGCCAAAGAAGGCGTTCCATCAGAAGGTCCTGAGACCAAGGTGGAGAACATTGGCGTGAA
  GACGGTCACGATCACATGGAAAGAGATTCCCAAGAGTGAGAGAAAGGGTATCATCTGCAACTACACCATC
  TTTTACCAAGCTGAAGGTGGAAAAGGATTCTCCAAGACAGTCAATTCCAGCATCTTGCAGTACGGCCTGG
  AGTCCCTGAAACGAAAGACCTCTTACATTGTTCAGGTCATGGCCAACACCAGTGCTGGGGGAACCAACGG
  GACCAGCATAAATTTCAAGACATTGTCATTCAGTGTCTTTGAGATTATCCTCATAACTTCTCTGATTGGT
  GGAGGCCTTCTTATTCTCATTATCCTGACAGTGGCATATGGTCTCAAAAAACCCAACAAATTGACTCATC
  TGTGTTGGCCCACCGTTCCCAACCCTGCTGAAAGTAGTATAGCCACATGGCATGGAGATGATTTCAAGGA
  TAAGCTAAACCTGAAGGAGTCTGATGACTCTGTGAACACAGAAGACAGGATCTTAAAACCATGTTCCACC
  CCCAGTGACAAGTTGGTGATTGACAAGTTGGTGGTGAACTTTGGGAATGTTCTGCAAGAAATTTTCACAG
  ATGAAGCCAGAACGGGTCAGGAAAACAATTTAGGAGGGGAAAAGAATGGGTATGTGACCTGCCCCTTCAG
  GCCTGATTGTCCCCTGGGGAAAAGTTTTGAGGAGCTCCCAGTTTCACCTGAGATTCCGCCCAGAAAATCC
  CAATACCTACGTTCGAGGATGCCAGAGGGGACCCGCCCAGAAGCCAAAGAGCAGCTTCTCTTTTCTGGTC
  AAAGTTTAGTACCAGATCATCTGTGTGAGGAAGGAGCCCCAAATCCATATTTGAAAAATTCAGTGACAGC
  CAGGGAATTTCTTGTGTCTGAAAAACTTCCAGAGCACACCAAGGGAGAAGTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC218212 representing NM_139017
  Red=Cloning site Green=Tags(s)
  
  MCIRQLKFFTTACVCECPQNILSPQPSCVNLGMMWTWALWMLPSLCKFSLAALPAKPENISCVYYYRKNL
  TCTWSPGKETSYTQYTVKRTYAFGEKHDNCTTNSSTSENRASCSFFLPRITIPDNYTIEVEAENGDGVIK
  SHMTYWRLENIAKTEPPKIFRVKPVLGIKRMIQIEWIKPELAPVSSDLKYTLRFRTVNSTSWMEVNFAKN
  RKDKNQTYNLTGLQPFTEYVIALRCAVKESKFWSDWSQEKMGMTEEEAPCGLELWRVLKPAEADGRRPVR
  LLWKKARGAPVLEKTLGYNIWYYPESNTNLTETMNTTNQQLELHLGGESFWVSMISYNSLGKSPVATLRI
  PAIQEKSFQCIEVMQACVAEDQLVVKWQSSALDVNTWMIEWFPDVDSEPTTLSWESVSQATNWTIQQDKL
  KPFWCYNISVYPMLHDKVGEPYSIQAYAKEGVPSEGPETKVENIGVKTVTITWKEIPKSERKGIICNYTI
  FYQAEGGKGFSKTVNSSILQYGLESLKRKTSYIVQVMANTSAGGTNGTSINFKTLSFSVFEIILITSLIG
  GGLLILIILTVAYGLKKPNKLTHLCWPTVPNPAESSIATWHGDDFKDKLNLKESDDSVNTEDRILKPCST
  PSDKLVIDKLVVNFGNVLQEIFTDEARTGQENNLGGEKNGYVTCPFRPDCPLGKSFEELPVSPEIPPRKS
  QYLRSRMPEGTRPEAKEQLLFSGQSLVPDHLCEEGAPNPYLKNSVTAREFLVSEKLPEHTKGEV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_139017
• ORF Size: 2292 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_139017.6
• RefSeq Size: 2393 bp
• RefSeq ORF: 2295 bp
• Locus ID: 133396
• UniProt ID: Q8NI17
• Cytogenetics: 5q11.2
• Protein Families: Druggable Genome, Transmembrane
• MW: 86.3 kDa
• Gene Summary: The protein encoded by this gene belongs to the type I cytokine receptor family. This receptor, with homology to gp130, is expressed on monocytes, and is involved in IL-31 signaling via activation of STAT-3 and STAT-5. It functions either as a monomer, or as part of a receptor complex with oncostatin M receptor (OSMR). Several alternatively spliced transcript variants encoding different isoforms have been noted for this gene.[provided by RefSeq, Jun 2011]