TTC8 (NM_144596) Human Tagged ORF Clone

CAT#: RC218089

TTC8 (Myc-DDK-tagged)-Human tetratricopeptide repeat domain 8 (TTC8), transcript variant 1

Product Images

Circular map for RC218089

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TTC8
• Synonyms: BBS8; RP51
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC218089 representing NM_144596
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCTCGGAGATGGAGCCGCTGCTCCTGGCCTGGAGCTATTTTAGGCGCAGGAAGTTCCAGCTCTGCG
  CCGATCTATGCACGCAGATGCTGGAGAAGTCCCCTTATGACCAGGAACCAGATCCTGAATTGCCAGTGCA
  TCAGGCAGCTTGGATCTTAAAAGCAAGAGCGCTAACAGAAATGGTATACATAGATGAAATTGATGTAGAT
  CAGGAAGGAATTGCAGAAATGATGCTGGATGAAAATGCTATAGCTCAAGTTCCACGCCCTGGAACGTCTT
  TGAAACTCCCTGGAACTAATCAGACAGGAGGGCCTAGCCAGGCCGTTAGGCCAATCACACAAGCTGGAAG
  ACCCATTACAGGTTTCCTCAGGCCCAGCACGCAGAGTGGAAGGCCAGGCACTATGGAACAGGCTATCAGA
  ACACCCAGAACCGCCTACACAGCCCGCCCTATCACCAGCTCCTCCGGAAGATTTGTCAGGCTGGGAACGG
  CTTCCATGCTTACAAGTCCTGATGGACCATTTATAAATTTATCTAGGCTGAATTTAACAAAGTATTCCCA
  GAAACCTAAGTTGGCAAAGGCTTTGTTTGAGTATATCTTTCATCATGAAAATGATGTTAAGACTGCTTTG
  GATCTGGCTGCCCTCTCCACAGAACATTCTCAGTACAAGGACTGGTGGTGGAAAGTACAGATTGGAAAAT
  GTTACTACAGGTTGGGAATGTATCGTGAAGCAGAAAAACAGTTTAAATCAGCCCTGAAGCAGCAGGAAAT
  GGTAGATACATTTCTGTACTTGGCAAAAGTTTATGTCTCATTGGATCAACCTGTGACTGCTTTAAATCTT
  TTCAAACAAGGCTTAGATAAGTTTCCAGGAGAAGTAACCCTGCTCTGTGGAATTGCAAGAATCTATGAGG
  AAATGAACAATATGTCATCAGCAGCAGAATATTACAAAGAAGTTTTGAAACAAGACAATACTCATGTGGA
  AGCCATCGCATGCATTGGAAGCAACCACTTCTATTCTGATCAGCCAGAAATAGCTCTCCGGTTTTACAGG
  CGGCTGCTGCAGATGGGCATTTATAACGGCCAGCTTTTTAACAATCTGGGGCTGTGTTGCTTCTATGCCC
  AGCAGTATGATATGACTCTGACCTCATTTGAACGTGCCCTTTCTTTGGCTGAAAATGAAGAAGAGGCAGC
  TGATGTCTGGTACAACTTGGGACATGTAGCTGTGGGAATAGGAGATACAAATTTGGCCCATCAGTGCTTC
  AGGCTGGCTCTGGTCAACAACAACAACCACGCCGAGGCCTACAACAACCTGGCTGTGCTGGAGATGCGGA
  AGGGCCACGTTGAACAGGCAAGGGCACTATTACAAACTGCATCATCATTAGCACCCCATATGTATGAACC
  GCATTTTAATTTTGCAACAATCTCTGATAAGATTGGAGATCTGCAGAGAAGCTATGTTGCTGCGCAGAAG
  TCTGAAGCAGCATTTCCAGACCATGTGGACACACAACATTTAATTAAACAATTAAGGCAGCATTTTGCTA
  TGCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC218089 representing NM_144596
  Red=Cloning site Green=Tags(s)
  
  MSSEMEPLLLAWSYFRRRKFQLCADLCTQMLEKSPYDQEPDPELPVHQAAWILKARALTEMVYIDEIDVD
  QEGIAEMMLDENAIAQVPRPGTSLKLPGTNQTGGPSQAVRPITQAGRPITGFLRPSTQSGRPGTMEQAIR
  TPRTAYTARPITSSSGRFVRLGTASMLTSPDGPFINLSRLNLTKYSQKPKLAKALFEYIFHHENDVKTAL
  DLAALSTEHSQYKDWWWKVQIGKCYYRLGMYREAEKQFKSALKQQEMVDTFLYLAKVYVSLDQPVTALNL
  FKQGLDKFPGEVTLLCGIARIYEEMNNMSSAAEYYKEVLKQDNTHVEAIACIGSNHFYSDQPEIALRFYR
  RLLQMGIYNGQLFNNLGLCCFYAQQYDMTLTSFERALSLAENEEEAADVWYNLGHVAVGIGDTNLAHQCF
  RLALVNNNNHAEAYNNLAVLEMRKGHVEQARALLQTASSLAPHMYEPHFNFATISDKIGDLQRSYVAAQK
  SEAAFPDHVDTQHLIKQLRQHFAML
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_144596
• ORF Size: 1545 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_144596.4
• RefSeq Size: 2248 bp
• RefSeq ORF: 1548 bp
• Locus ID: 123016
• UniProt ID: Q8TAM2
• Cytogenetics: 14q31.3
• Domains: TPR
• MW: 58.2 kDa
• Gene Summary: This gene encodes a protein that has been directly linked to Bardet-Biedl syndrome. The primary features of this syndrome include retinal dystrophy, obesity, polydactyly, renal abnormalities and learning disabilities. Experimentation in non-human eukaryotes suggests that this gene is expressed in ciliated cells and that it is involved in the formation of cilia. A mutation in this gene has also been implicated in nonsyndromic retinitis pigmentosa. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]