ZNF189 (NM_003452) Human Tagged ORF Clone
CAT#: RC217848
- TrueORF®
ZNF189 (Myc-DDK-tagged)-Human zinc finger protein 189 (ZNF189), transcript variant 1
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_003452" in other vectors (4)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | ZNF189 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC217848 representing NM_003452
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTTCCCCGAGCCCCCCGCCGGAGTCGAAGGGGTTGCTGACATTTGAGGATGTGGCTGTGTTTTTTA CCCAGGAGGAGTGGGATTATCTGGACCCAGCTCAGAGAAGCCTGTATAAAGATGTCATGATGGAGAATTA TGGAAACCTGGTCTCACTGGATGTTTTGAACAGAGATAAGGATGAGGAGCCAACTGTAAAACAAGAGATT GAAGAAATTGAGGAAGAAGTGGAACCACAGGGTGTAATAGTTACAAGAATCAAAAGTGAAATTGACCAGG ATCCTATGGGTAGAGAAACATTTGAACTTGTTGGTAGGTTAGATAAACAAAGAGGGATCTTCCTATGGGA AATACCAAGGGAATCTTTGACCCAGGAACAGAGAATGTTCAGAGAAAACACTAACATTATCCGTAAAAGA CCAAACTCAGAAGAGAAATGCCATAAATGTGAAGAATGTGGAAAGGGTTTTGTCCGCAAGGCCCATTTCA TTCAACATCAAAGGGTCCATACTGGTGAGAAACCTTTTCAGTGCAATGAATGTGGGAAAAGTTTTAGTCG CAGTTCATTTGTTATTGAACATCAGAGAATTCACACTGGGGAAAGGCCCTATGAGTGTAATTACTGTGGA AAAACCTTTAGTGTGAGCTCAACCCTTATTAGACATCAGAGAATCCACACTGGAGAAAGACCCTATCAGT GTAATCAGTGTAAACAGAGCTTCAGCCAGAGAAGGAGCCTTGTTAAACATCAAAGGATTCATACAGGTGA GAAACCCCATAAATGTAGTGACTGTGGGAAAGCCTTCAGTTGGAAATCACACCTTATTGAGCATCAAAGA ACTCACACTGGTGAGAAACCTTATCACTGTACCAAATGTAAGAAGAGCTTTAGTCGAAATTCATTGCTTG TTGAGCATCAAAGAATTCACACTGGGGAAAGACCCCATAAATGTGGTGAATGTGGGAAAGCCTTTCGATT AAGCACATACCTTATACAACACCAAAAAATTCACACTGGCGAGAAGCCTTTTCTTTGTATTGAGTGTGGA AAAAGTTTCAGTCGGAGCTCATTCCTTATTGAACATCAGAGGATCCATACTGGTGAAAGACCTTATCAGT GCAAAGAGTGTGGGAAAAGTTTCAGTCAGCTTTGCAACCTTACTCGTCATCAGAGAATTCACACAGGAGA CAAGCCCCATAAATGTGAGGAATGTGGAAAAGCCTTTAGTAGAAGCTCAGGTCTTATTCAGCATCAGAGA ATTCACACCAGGGAGAAGACTTATCCATACAATGAAACTAAGGAAAGTTTTGATCCAAATTGCAGTCTTG TTATACAGCAGGAAGTCTACCCTAAGGAGAAATCTTATAAATGTGATGAATGTGGGAAAACTTTTAGTGT TAGTGCTCATCTTGTACAACATCAAAGAATCCACACTGGTGAAAAGCCCTATCTATGTACTGTCTGTGGG AAAAGCTTCAGCCGGAGCTCATTTCTTATTGAACATCAGAGAATCCACACTGGTGAGAGACCCTATCTGT GCAGACAGTGTGGAAAAAGCTTTAGTCAGCTTTGTAATCTTATTCGACATCAGGGTGTTCACACAGGTAA TAAACCCCATAAATGTGATGAATGTGGAAAGGCCTTTAGCCGGAACTCGGGTCTTATTCAGCATCAGAGA ATACACACAGGAGAGAAACCTTATAAGTGTGAGAAGTGCGACAAAAGTTTCAGTCAACAGCGCAGTCTTG TCAACCATCAGAAGATCCATGCAGAGGTGAAAACCCAAGAAACCCATGAATGTGACGCTTGTGGTGAAGC CTTTAATTGCCGTATTTCTCTTATTCAGCATCAGAAATTGCACACAGCATGGATGCAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC217848 representing NM_003452
Red=Cloning site Green=Tags(s) MASPSPPPESKGLLTFEDVAVFFTQEEWDYLDPAQRSLYKDVMMENYGNLVSLDVLNRDKDEEPTVKQEI EEIEEEVEPQGVIVTRIKSEIDQDPMGRETFELVGRLDKQRGIFLWEIPRESLTQEQRMFRENTNIIRKR PNSEEKCHKCEECGKGFVRKAHFIQHQRVHTGEKPFQCNECGKSFSRSSFVIEHQRIHTGERPYECNYCG KTFSVSSTLIRHQRIHTGERPYQCNQCKQSFSQRRSLVKHQRIHTGEKPHKCSDCGKAFSWKSHLIEHQR THTGEKPYHCTKCKKSFSRNSLLVEHQRIHTGERPHKCGECGKAFRLSTYLIQHQKIHTGEKPFLCIECG KSFSRSSFLIEHQRIHTGERPYQCKECGKSFSQLCNLTRHQRIHTGDKPHKCEECGKAFSRSSGLIQHQR IHTREKTYPYNETKESFDPNCSLVIQQEVYPKEKSYKCDECGKTFSVSAHLVQHQRIHTGEKPYLCTVCG KSFSRSSFLIEHQRIHTGERPYLCRQCGKSFSQLCNLIRHQGVHTGNKPHKCDECGKAFSRNSGLIQHQR IHTGEKPYKCEKCDKSFSQQRSLVNHQKIHAEVKTQETHECDACGEAFNCRISLIQHQKLHTAWMQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
![]() |
ACCN | NM_003452 |
ORF Size | 1878 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_003452.4 |
RefSeq Size | 3186 bp |
RefSeq ORF | 1881 bp |
Locus ID | 7743 |
UniProt ID | O75820 |
Cytogenetics | 9q31.1 |
Domains | KRAB, zf-C2H2 |
Protein Families | Transcription Factors |
MW | 72.8 kDa |
Gene Summary | Kruppel-like zinc finger proteins such as ZNF189 contain a conserved stretch of 7 amino acids that connects a variable number of DNA-binding zinc finger repeats of the cys(2)his(2) (C2H2) type (summarized by Odeberg et al., 1998 [PubMed 9653648]). Approximately 30% of human Kruppel-like zinc finger proteins contain an N-terminal Kruppel-associated box (KRAB) domain. The KRAB domain consists of approximately 75 amino acids that may be subdivided into an A box, which is present in every KRAB domain and is essential for transcriptional repression, and a B box, which is not always present.[supplied by OMIM, May 2010] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC217848L3 | Lenti-ORF clone of ZNF189 (Myc-DDK-tagged)-Human zinc finger protein 189 (ZNF189), transcript variant 1 |
USD 883.00 |
|
RC217848L4 | Lenti-ORF clone of ZNF189 (mGFP-tagged)-Human zinc finger protein 189 (ZNF189), transcript variant 1 |
USD 883.00 |
|
RG217848 | ZNF189 (tGFP-tagged) - Human zinc finger protein 189 (ZNF189), transcript variant 1 |
USD 783.00 |
|
SC303308 | ZNF189 (untagged)-Human zinc finger protein 189 (ZNF189), transcript variant 1 |
USD 642.00 |
{0} Product Review(s)
Be the first one to submit a review