ELOF1 (NM_032377) Human Tagged ORF Clone

CAT#: RC217106

ELOF1 (Myc-DDK-tagged)-Human elongation factor 1 homolog (S. cerevisiae) (ELOF1)

Product Images

Circular map for RC217106

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  Western blot validation of overexpression lysate (Cat# LY410170) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC217106 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified ELOF1 protein (Cat# TP317106). The protein was produced from HEK293T cells transfected with ELOF1 cDNA clone (Cat# RC217106) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ELOF1
• Synonyms: ELF1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC217106 representing NM_032377
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGCGCAGAAAGTCAAAACGAAAGCCGCCTCCCAAGAAGAAGATGACAGGCACCCTCGAGACCCAGT
  TCACCTGCCCCTTCTGCAACCACGAGAAATCCTGTGATGTGAAAATGGACCGTGCCCGCAACACCGGAGT
  CATCTCTTGTACCGTGTGCCTAGAGGAATTCCAGACGCCCATAACGTATCTGTCAGAACCCGTGGATGTG
  TACAGTGATTGGATAGACGCCTGCGAGGCGGCCAATCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC217106 representing NM_032377
  Red=Cloning site Green=Tags(s)
  
  MGRRKSKRKPPPKKKMTGTLETQFTCPFCNHEKSCDVKMDRARNTGVISCTVCLEEFQTPITYLSEPVDV
  YSDWIDACEAANQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032377
• ORF Size: 249 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032377.3
• RefSeq Size: 982 bp
• RefSeq ORF: 252 bp
• Locus ID: 84337
• UniProt ID: P60002
• Cytogenetics: 19p13.2
• Domains: DUF701
• MW: 9.3 kDa
• Gene Summary: Transcription elongation factor implicated in the maintenance of proper chromatin structure in actively transcribed regions.[UniProtKB/Swiss-Prot Function]