Tankyrase 2 (TNKS2) (NM_025235) Human Tagged ORF Clone

CAT#: RC214654

TNKS2 (Myc-DDK-tagged)-Human tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase 2 (TNKS2)

Product Images

Circular map for RC214654

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tankyrase 2
• Synonyms: ARTD6; PARP-5b; PARP-5c; PARP5B; PARP5C; pART6; TANK2; TNKL
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214654 representing NM_025235
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGGGTCGCCGCTGCGCCGGCGGGGGAGCGGCCTGCGCGAGCGCCGCGGCCGAGGCCGTGGAGCCGG
  CCGCCCGAGAGCTGTTCGAGGCGTGCCGCAACGGGGACGTGGAACGAGTCAAGAGGCTGGTGACGCCTGA
  GAAGGTGAACAGCCGCGACACGGCGGGCAGGAAATCCACCCCGCTGCACTTCGCCGCAGGTTTTGGGCGG
  AAAGACGTAGTTGAATATTTGCTTCAGAATGGTGCAAATGTCCAAGCACGTGATGATGGGGGCCTTATTC
  CTCTTCATAATGCATGCTCTTTTGGTCATGCTGAAGTAGTCAATCTCCTTTTGCGACATGGTGCAGACCC
  CAATGCTCGAGATAATTGGAATTATACTCCTCTCCATGAAGCTGCAATTAAAGGAAAGATTGATGTTTGC
  ATTGTGCTGTTACAGCATGGAGCTGAGCCAACCATCCGAAATACAGATGGAAGGACAGCATTGGATTTAG
  CAGATCCATCTGCCAAAGCAGTGCTTACTGGTGAATATAAGAAAGATGAACTCTTAGAAAGTGCCAGGAG
  TGGCAATGAAGAAAAAATGATGGCTCTACTCACACCATTAAATGTCAACTGCCACGCAAGTGATGGCAGA
  AAGTCAACTCCATTACATTTGGCAGCAGGATATAACAGAGTAAAGATTGTACAGCTGTTACTGCAACATG
  GAGCTGATGTCCATGCTAAAGATAAAGGTGATCTGGTACCATTACACAATGCCTGTTCTTATGGTCATTA
  TGAAGTAACTGAACTTTTGGTCAAGCATGGTGCCTGTGTAAATGCAATGGACTTGTGGCAATTCACTCCT
  CTTCATGAGGCAGCTTCTAAGAACAGGGTTGAAGTATGTTCTCTTCTCTTAAGTTATGGTGCAGACCCAA
  CACTGCTCAATTGTCACAATAAAAGTGCTATAGACTTGGCTCCCACACCACAGTTAAAAGAAAGATTAGC
  ATATGAATTTAAAGGCCACTCGTTGCTGCAAGCTGCACGAGAAGCTGATGTTACTCGAATCAAAAAACAT
  CTCTCTCTGGAAATGGTGAATTTCAAGCATCCTCAAACACATGAAACAGCATTGCATTGTGCTGCTGCAT
  CTCCATATCCCAAAAGAAAGCAAATATGTGAACTGTTGCTAAGAAAAGGAGCAAACATCAATGAAAAGAC
  TAAAGAATTCTTGACTCCTCTGCACGTGGCATCTGAGAAAGCTCATAATGATGTTGTTGAAGTAGTGGTG
  AAACATGAAGCAAAGGTTAATGCTCTGGATAATCTTGGTCAGACTTCTCTACACAGAGCTGCATATTGTG
  GTCATCTACAAACCTGCCGCCTACTCCTGAGCTATGGGTGTGATCCTAACATTATATCCCTTCAGGGCTT
  TACTGCTTTACAGATGGGAAATGAAAATGTACAGCAACTCCTCCAAGAGGGTATCTCATTAGGTAATTCA
  GAGGCAGACAGACAATTGCTGGAAGCTGCAAAGGCTGGAGATGTCGAAACTGTAAAAAAACTGTGTACTG
  TTCAGAGTGTCAACTGCAGAGACATTGAAGGGCGTCAGTCTACACCACTTCATTTTGCAGCTGGGTATAA
  CAGAGTGTCCGTGGTGGAATATCTGCTACAGCATGGAGCTGATGTGCATGCTAAAGATAAAGGAGGCCTT
  GTACCTTTGCACAATGCATGTTCTTATGGACATTATGAAGTTGCAGAACTTCTTGTTAAACATGGAGCAG
  TAGTTAATGTAGCTGATTTATGGAAATTTACACCTTTACATGAAGCAGCAGCAAAAGGAAAATATGAAAT
  TTGCAAACTTCTGCTCCAGCATGGTGCAGACCCTACAAAAAAAAACAGGGATGGAAATACTCCTTTGGAT
  CTTGTTAAAGATGGAGATACAGATATTCAAGATCTGCTTAGGGGAGATGCAGCTTTGCTAGATGCTGCCA
  AGAAGGGTTGTTTAGCCAGAGTGAAGAAGTTGTCTTCTCCTGATAATGTAAATTGCCGCGATACCCAAGG
  CAGACATTCAACACCTTTACATTTAGCAGCTGGTTATAATAATTTAGAAGTTGCAGAGTATTTGTTACAA
  CACGGAGCTGATGTGAATGCCCAAGACAAAGGAGGACTTATTCCTTTACATAATGCAGCATCTTACGGGC
  ATGTAGATGTAGCAGCTCTACTAATAAAGTATAATGCATGTGTCAATGCCACGGACAAATGGGCTTTCAC
  ACCTTTGCACGAAGCAGCCCAAAAGGGACGAACACAGCTTTGTGCTTTGTTGCTAGCCCATGGAGCTGAC
  CCGACTCTTAAAAATCAGGAAGGACAAACACCTTTAGATTTAGTTTCAGCAGATGATGTCAGCGCTCTTC
  TGACAGCAGCCATGCCCCCATCTGCTCTGCCCTCTTGTTACAAGCCTCAAGTGCTCAATGGTGTGAGAAG
  CCCAGGAGCCACTGCAGATGCTCTCTCTTCAGGTCCATCTAGCCCATCAAGCCTTTCTGCAGCCAGCAGT
  CTTGACAACTTATCTGGGAGTTTTTCAGAACTGTCTTCAGTAGTTAGTTCAAGTGGAACAGAGGGTGCTT
  CCAGTTTGGAGAAAAAGGAGGTTCCAGGAGTAGATTTTAGCATAACTCAATTCGTAAGGAATCTTGGACT
  TGAGCACCTAATGGATATATTTGAGAGAGAACAGATCACTTTGGATGTATTAGTTGAGATGGGGCACAAG
  GAGCTGAAGGAGATTGGAATCAATGCTTATGGACATAGGCACAAACTAATTAAAGGAGTCGAGAGACTTA
  TCTCCGGACAACAAGGTCTTAACCCATATTTAACTTTGAACACCTCTGGTAGTGGAACAATTCTTATAGA
  TCTGTCTCCTGATGATAAAGAGTTTCAGTCTGTGGAGGAAGAGATGCAAAGTACAGTTCGAGAGCACAGA
  GATGGAGGTCATGCAGGTGGAATCTTCAACAGATACAATATTCTCAAGATTCAGAAGGTTTGTAACAAGA
  AACTATGGGAAAGATACACTCACCGGAGAAAAGAAGTTTCTGAAGAAAACCACAACCATGCCAATGAACG
  AATGCTATTTCATGGGTCTCCTTTTGTGAATGCAATTATCCACAAAGGCTTTGATGAAAGGCATGCGTAC
  ATAGGTGGTATGTTTGGAGCTGGCATTTATTTTGCTGAAAACTCTTCCAAAAGCAATCAATATGTATATG
  GAATTGGAGGAGGTACTGGGTGTCCAGTTCACAAAGACAGATCTTGTTACATTTGCCACAGGCAGCTGCT
  CTTTTGCCGGGTAACCTTGGGAAAGTCTTTCCTGCAGTTCAGTGCAATGAAAATGGCACATTCTCCTCCA
  GGTCATCACTCAGTCACTGGTAGGCCCAGTGTAAATGGCCTAGCATTAGCTGAATATGTTATTTACAGAG
  GAGAACAGGCTTATCCTGAGTATTTAATTACTTACCAGATTATGAGGCCTGAAGGTATGGTCGATGGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214654 representing NM_025235
  Red=Cloning site Green=Tags(s)
  
  MSGRRCAGGGAACASAAAEAVEPAARELFEACRNGDVERVKRLVTPEKVNSRDTAGRKSTPLHFAAGFGR
  KDVVEYLLQNGANVQARDDGGLIPLHNACSFGHAEVVNLLLRHGADPNARDNWNYTPLHEAAIKGKIDVC
  IVLLQHGAEPTIRNTDGRTALDLADPSAKAVLTGEYKKDELLESARSGNEEKMMALLTPLNVNCHASDGR
  KSTPLHLAAGYNRVKIVQLLLQHGADVHAKDKGDLVPLHNACSYGHYEVTELLVKHGACVNAMDLWQFTP
  LHEAASKNRVEVCSLLLSYGADPTLLNCHNKSAIDLAPTPQLKERLAYEFKGHSLLQAAREADVTRIKKH
  LSLEMVNFKHPQTHETALHCAAASPYPKRKQICELLLRKGANINEKTKEFLTPLHVASEKAHNDVVEVVV
  KHEAKVNALDNLGQTSLHRAAYCGHLQTCRLLLSYGCDPNIISLQGFTALQMGNENVQQLLQEGISLGNS
  EADRQLLEAAKAGDVETVKKLCTVQSVNCRDIEGRQSTPLHFAAGYNRVSVVEYLLQHGADVHAKDKGGL
  VPLHNACSYGHYEVAELLVKHGAVVNVADLWKFTPLHEAAAKGKYEICKLLLQHGADPTKKNRDGNTPLD
  LVKDGDTDIQDLLRGDAALLDAAKKGCLARVKKLSSPDNVNCRDTQGRHSTPLHLAAGYNNLEVAEYLLQ
  HGADVNAQDKGGLIPLHNAASYGHVDVAALLIKYNACVNATDKWAFTPLHEAAQKGRTQLCALLLAHGAD
  PTLKNQEGQTPLDLVSADDVSALLTAAMPPSALPSCYKPQVLNGVRSPGATADALSSGPSSPSSLSAASS
  LDNLSGSFSELSSVVSSSGTEGASSLEKKEVPGVDFSITQFVRNLGLEHLMDIFEREQITLDVLVEMGHK
  ELKEIGINAYGHRHKLIKGVERLISGQQGLNPYLTLNTSGSGTILIDLSPDDKEFQSVEEEMQSTVREHR
  DGGHAGGIFNRYNILKIQKVCNKKLWERYTHRRKEVSEENHNHANERMLFHGSPFVNAIIHKGFDERHAY
  IGGMFGAGIYFAENSSKSNQYVYGIGGGTGCPVHKDRSCYICHRQLLFCRVTLGKSFLQFSAMKMAHSPP
  GHHSVTGRPSVNGLALAEYVIYRGEQAYPEYLITYQIMRPEGMVDG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_025235
• ORF Size: 3498 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025235.2, NP_079511.1
• RefSeq Size: 6189 bp
• RefSeq ORF: 3501 bp
• Locus ID: 80351
• UniProt ID: Q9H2K2
• Cytogenetics: 10q23.32
• Domains: SAM, ANK
• MW: 126.7 kDa
• Gene Summary: Poly-ADP-ribosyltransferase involved in various processes such as Wnt signaling pathway, telomere length and vesicle trafficking (PubMed:11739745, PubMed:11802774, PubMed:19759537, PubMed:21478859, PubMed:23622245, PubMed:25043379). Acts as an activator of the Wnt signaling pathway by mediating poly-ADP-ribosylation of AXIN1 and AXIN2, 2 key components of the beta-catenin destruction complex: poly-ADP-ribosylated target proteins are recognized by RNF146, which mediates their ubiquitination and subsequent degradation (PubMed:19759537, PubMed:21478859). Also mediates poly-ADP-ribosylation of BLZF1 and CASC3, followed by recruitment of RNF146 and subsequent ubiquitination (PubMed:21478859). Mediates poly-ADP-ribosylation of TERF1, thereby contributing to the regulation of telomere length (PubMed:11739745). Stimulates 26S proteasome activity (PubMed:23622245).[UniProtKB/Swiss-Prot Function]