Tankyrase 2 (TNKS2) (NM_025235) Human Tagged ORF Clone
CAT#: RC214654
- TrueORF®
TNKS2 (Myc-DDK-tagged)-Human tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase 2 (TNKS2)
ORF Plasmid: tGFP
Lentiviral Particles: DDK DDK w/ Puro mGFP mGFP w/ Puro
"NM_025235" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Tankyrase 2 |
Synonyms | ARTD6; PARP-5b; PARP-5c; PARP5B; PARP5C; pART6; TANK2; TNKL |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC214654 representing NM_025235
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGGGTCGCCGCTGCGCCGGCGGGGGAGCGGCCTGCGCGAGCGCCGCGGCCGAGGCCGTGGAGCCGG CCGCCCGAGAGCTGTTCGAGGCGTGCCGCAACGGGGACGTGGAACGAGTCAAGAGGCTGGTGACGCCTGA GAAGGTGAACAGCCGCGACACGGCGGGCAGGAAATCCACCCCGCTGCACTTCGCCGCAGGTTTTGGGCGG AAAGACGTAGTTGAATATTTGCTTCAGAATGGTGCAAATGTCCAAGCACGTGATGATGGGGGCCTTATTC CTCTTCATAATGCATGCTCTTTTGGTCATGCTGAAGTAGTCAATCTCCTTTTGCGACATGGTGCAGACCC CAATGCTCGAGATAATTGGAATTATACTCCTCTCCATGAAGCTGCAATTAAAGGAAAGATTGATGTTTGC ATTGTGCTGTTACAGCATGGAGCTGAGCCAACCATCCGAAATACAGATGGAAGGACAGCATTGGATTTAG CAGATCCATCTGCCAAAGCAGTGCTTACTGGTGAATATAAGAAAGATGAACTCTTAGAAAGTGCCAGGAG TGGCAATGAAGAAAAAATGATGGCTCTACTCACACCATTAAATGTCAACTGCCACGCAAGTGATGGCAGA AAGTCAACTCCATTACATTTGGCAGCAGGATATAACAGAGTAAAGATTGTACAGCTGTTACTGCAACATG GAGCTGATGTCCATGCTAAAGATAAAGGTGATCTGGTACCATTACACAATGCCTGTTCTTATGGTCATTA TGAAGTAACTGAACTTTTGGTCAAGCATGGTGCCTGTGTAAATGCAATGGACTTGTGGCAATTCACTCCT CTTCATGAGGCAGCTTCTAAGAACAGGGTTGAAGTATGTTCTCTTCTCTTAAGTTATGGTGCAGACCCAA CACTGCTCAATTGTCACAATAAAAGTGCTATAGACTTGGCTCCCACACCACAGTTAAAAGAAAGATTAGC ATATGAATTTAAAGGCCACTCGTTGCTGCAAGCTGCACGAGAAGCTGATGTTACTCGAATCAAAAAACAT CTCTCTCTGGAAATGGTGAATTTCAAGCATCCTCAAACACATGAAACAGCATTGCATTGTGCTGCTGCAT CTCCATATCCCAAAAGAAAGCAAATATGTGAACTGTTGCTAAGAAAAGGAGCAAACATCAATGAAAAGAC TAAAGAATTCTTGACTCCTCTGCACGTGGCATCTGAGAAAGCTCATAATGATGTTGTTGAAGTAGTGGTG AAACATGAAGCAAAGGTTAATGCTCTGGATAATCTTGGTCAGACTTCTCTACACAGAGCTGCATATTGTG GTCATCTACAAACCTGCCGCCTACTCCTGAGCTATGGGTGTGATCCTAACATTATATCCCTTCAGGGCTT TACTGCTTTACAGATGGGAAATGAAAATGTACAGCAACTCCTCCAAGAGGGTATCTCATTAGGTAATTCA GAGGCAGACAGACAATTGCTGGAAGCTGCAAAGGCTGGAGATGTCGAAACTGTAAAAAAACTGTGTACTG TTCAGAGTGTCAACTGCAGAGACATTGAAGGGCGTCAGTCTACACCACTTCATTTTGCAGCTGGGTATAA CAGAGTGTCCGTGGTGGAATATCTGCTACAGCATGGAGCTGATGTGCATGCTAAAGATAAAGGAGGCCTT GTACCTTTGCACAATGCATGTTCTTATGGACATTATGAAGTTGCAGAACTTCTTGTTAAACATGGAGCAG TAGTTAATGTAGCTGATTTATGGAAATTTACACCTTTACATGAAGCAGCAGCAAAAGGAAAATATGAAAT TTGCAAACTTCTGCTCCAGCATGGTGCAGACCCTACAAAAAAAAACAGGGATGGAAATACTCCTTTGGAT CTTGTTAAAGATGGAGATACAGATATTCAAGATCTGCTTAGGGGAGATGCAGCTTTGCTAGATGCTGCCA AGAAGGGTTGTTTAGCCAGAGTGAAGAAGTTGTCTTCTCCTGATAATGTAAATTGCCGCGATACCCAAGG CAGACATTCAACACCTTTACATTTAGCAGCTGGTTATAATAATTTAGAAGTTGCAGAGTATTTGTTACAA CACGGAGCTGATGTGAATGCCCAAGACAAAGGAGGACTTATTCCTTTACATAATGCAGCATCTTACGGGC ATGTAGATGTAGCAGCTCTACTAATAAAGTATAATGCATGTGTCAATGCCACGGACAAATGGGCTTTCAC ACCTTTGCACGAAGCAGCCCAAAAGGGACGAACACAGCTTTGTGCTTTGTTGCTAGCCCATGGAGCTGAC CCGACTCTTAAAAATCAGGAAGGACAAACACCTTTAGATTTAGTTTCAGCAGATGATGTCAGCGCTCTTC TGACAGCAGCCATGCCCCCATCTGCTCTGCCCTCTTGTTACAAGCCTCAAGTGCTCAATGGTGTGAGAAG CCCAGGAGCCACTGCAGATGCTCTCTCTTCAGGTCCATCTAGCCCATCAAGCCTTTCTGCAGCCAGCAGT CTTGACAACTTATCTGGGAGTTTTTCAGAACTGTCTTCAGTAGTTAGTTCAAGTGGAACAGAGGGTGCTT CCAGTTTGGAGAAAAAGGAGGTTCCAGGAGTAGATTTTAGCATAACTCAATTCGTAAGGAATCTTGGACT TGAGCACCTAATGGATATATTTGAGAGAGAACAGATCACTTTGGATGTATTAGTTGAGATGGGGCACAAG GAGCTGAAGGAGATTGGAATCAATGCTTATGGACATAGGCACAAACTAATTAAAGGAGTCGAGAGACTTA TCTCCGGACAACAAGGTCTTAACCCATATTTAACTTTGAACACCTCTGGTAGTGGAACAATTCTTATAGA TCTGTCTCCTGATGATAAAGAGTTTCAGTCTGTGGAGGAAGAGATGCAAAGTACAGTTCGAGAGCACAGA GATGGAGGTCATGCAGGTGGAATCTTCAACAGATACAATATTCTCAAGATTCAGAAGGTTTGTAACAAGA AACTATGGGAAAGATACACTCACCGGAGAAAAGAAGTTTCTGAAGAAAACCACAACCATGCCAATGAACG AATGCTATTTCATGGGTCTCCTTTTGTGAATGCAATTATCCACAAAGGCTTTGATGAAAGGCATGCGTAC ATAGGTGGTATGTTTGGAGCTGGCATTTATTTTGCTGAAAACTCTTCCAAAAGCAATCAATATGTATATG GAATTGGAGGAGGTACTGGGTGTCCAGTTCACAAAGACAGATCTTGTTACATTTGCCACAGGCAGCTGCT CTTTTGCCGGGTAACCTTGGGAAAGTCTTTCCTGCAGTTCAGTGCAATGAAAATGGCACATTCTCCTCCA GGTCATCACTCAGTCACTGGTAGGCCCAGTGTAAATGGCCTAGCATTAGCTGAATATGTTATTTACAGAG GAGAACAGGCTTATCCTGAGTATTTAATTACTTACCAGATTATGAGGCCTGAAGGTATGGTCGATGGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC214654 representing NM_025235
Red=Cloning site Green=Tags(s) MSGRRCAGGGAACASAAAEAVEPAARELFEACRNGDVERVKRLVTPEKVNSRDTAGRKSTPLHFAAGFGR KDVVEYLLQNGANVQARDDGGLIPLHNACSFGHAEVVNLLLRHGADPNARDNWNYTPLHEAAIKGKIDVC IVLLQHGAEPTIRNTDGRTALDLADPSAKAVLTGEYKKDELLESARSGNEEKMMALLTPLNVNCHASDGR KSTPLHLAAGYNRVKIVQLLLQHGADVHAKDKGDLVPLHNACSYGHYEVTELLVKHGACVNAMDLWQFTP LHEAASKNRVEVCSLLLSYGADPTLLNCHNKSAIDLAPTPQLKERLAYEFKGHSLLQAAREADVTRIKKH LSLEMVNFKHPQTHETALHCAAASPYPKRKQICELLLRKGANINEKTKEFLTPLHVASEKAHNDVVEVVV KHEAKVNALDNLGQTSLHRAAYCGHLQTCRLLLSYGCDPNIISLQGFTALQMGNENVQQLLQEGISLGNS EADRQLLEAAKAGDVETVKKLCTVQSVNCRDIEGRQSTPLHFAAGYNRVSVVEYLLQHGADVHAKDKGGL VPLHNACSYGHYEVAELLVKHGAVVNVADLWKFTPLHEAAAKGKYEICKLLLQHGADPTKKNRDGNTPLD LVKDGDTDIQDLLRGDAALLDAAKKGCLARVKKLSSPDNVNCRDTQGRHSTPLHLAAGYNNLEVAEYLLQ HGADVNAQDKGGLIPLHNAASYGHVDVAALLIKYNACVNATDKWAFTPLHEAAQKGRTQLCALLLAHGAD PTLKNQEGQTPLDLVSADDVSALLTAAMPPSALPSCYKPQVLNGVRSPGATADALSSGPSSPSSLSAASS LDNLSGSFSELSSVVSSSGTEGASSLEKKEVPGVDFSITQFVRNLGLEHLMDIFEREQITLDVLVEMGHK ELKEIGINAYGHRHKLIKGVERLISGQQGLNPYLTLNTSGSGTILIDLSPDDKEFQSVEEEMQSTVREHR DGGHAGGIFNRYNILKIQKVCNKKLWERYTHRRKEVSEENHNHANERMLFHGSPFVNAIIHKGFDERHAY IGGMFGAGIYFAENSSKSNQYVYGIGGGTGCPVHKDRSCYICHRQLLFCRVTLGKSFLQFSAMKMAHSPP GHHSVTGRPSVNGLALAEYVIYRGEQAYPEYLITYQIMRPEGMVDG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_025235 |
ORF Size | 3498 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_025235.2, NP_079511.1 |
RefSeq Size | 6189 bp |
RefSeq ORF | 3501 bp |
Locus ID | 80351 |
UniProt ID | Q9H2K2 |
Cytogenetics | 10q23.32 |
Domains | SAM, ANK |
MW | 126.7 kDa |
Gene Summary | Poly-ADP-ribosyltransferase involved in various processes such as Wnt signaling pathway, telomere length and vesicle trafficking (PubMed:11739745, PubMed:11802774, PubMed:19759537, PubMed:21478859, PubMed:23622245, PubMed:25043379). Acts as an activator of the Wnt signaling pathway by mediating poly-ADP-ribosylation of AXIN1 and AXIN2, 2 key components of the beta-catenin destruction complex: poly-ADP-ribosylated target proteins are recognized by RNF146, which mediates their ubiquitination and subsequent degradation (PubMed:19759537, PubMed:21478859). Also mediates poly-ADP-ribosylation of BLZF1 and CASC3, followed by recruitment of RNF146 and subsequent ubiquitination (PubMed:21478859). Mediates poly-ADP-ribosylation of TERF1, thereby contributing to the regulation of telomere length (PubMed:11739745). Stimulates 26S proteasome activity (PubMed:23622245).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC214654L1 | Lenti-ORF clone of TNKS2 (Myc-DDK-tagged)-Human tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase 2 (TNKS2) |
USD 1,822.00 |
|
RC214654L2 | Lenti-ORF clone of TNKS2 (mGFP-tagged)-Human tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase 2 (TNKS2) |
USD 1,822.00 |
|
RC214654L3 | Lenti-ORF clone of TNKS2 (Myc-DDK-tagged)-Human tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase 2 (TNKS2) |
USD 1,822.00 |
|
RC214654L4 | Lenti-ORF clone of TNKS2 (mGFP-tagged)-Human tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase 2 (TNKS2) |
USD 1,822.00 |
|
RG214654 | TNKS2 (tGFP-tagged) - Human tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase 2 (TNKS2) |
USD 1,722.00 |
|
SC110047 | TNKS2 (untagged)-Human tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase 2 (TNKS2) |
USD 1,523.00 |
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