Pannexin 2 (PANX2) (NM_052839) Human Tagged ORF Clone

CAT#: RC213641

PANX2 (Myc-DDK-tagged)-Human pannexin 2 (PANX2), transcript variant 1

Product Images

Circular map for RC213641

• 

  Western blot validation of overexpression lysate (Cat# LY409450) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC213641 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified PANX2 protein (Cat# TP313641). The protein was produced from HEK293T cells transfected with PANX2 cDNA clone (Cat# RC213641) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pannexin 2
• Synonyms: hPANX2; PX2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC213641 representing NM_052839
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCACCACCTCCTGGAGCAGTCGGCGGACATGGCGACCGCGCTGCTGGCGGGAGAGAAGCTGCGGGAGC
  TGATCCTGCCGGGCGCGCAGGACGACAAGGCGGGCGCGCTGGCCGCGCTGCTTCTGCAGCTGAAGCTGGA
  GCTGCCGTTCGACCGGGTGGTCACCATCGGCACCGTGCTGGTGCCCATCCTGCTGGTCACCCTGGTCTTC
  ACCAAGAACTTCGCAGAGGAACCCATTTACTGTTACACCCCGCACAACTTCACGCGCGACCAGGCGCTGT
  ACGCCCGCGGCTACTGCTGGACGGAGCTGCGGGACGCGCTGCCCGGCGTGGACGCCAGCCTGTGGCCGTC
  GCTGTTTGAGCACAAGTTCCTGCCCTACGCGCTGCTGGCCTTCGCCGCCATCATGTACGTGCCCGCGCTG
  GGCTGGGAGTTCCTGGCCTCCACGCGCCTCACCTCCGAGCTCAACTTCCTGCTGCAGGAGATCGACAACT
  GTTACCACCGGGCGGCCGAGGGCCGCGCGCCCAAGATCGAGAAGCAGATCCAGTCCAAGGGCCCGGGCAT
  CACGGAGCGCGAGAAGCGCGAGATCATCGAGAACGCGGAGAAGGAGAAGAGCCCGGAGCAGAACCTGTTC
  GAGAAGTACCTGGAGCGCCGCGGCCGCAGCAACTTCCTGGCCAAGCTGTACCTGGCGCGGCACGTGCTGA
  TCCTGCTGCTGAGCGCCGTGCCCATCTCCTACCTGTGCACCTACTACGCCACGCAGAAGCAGAACGAGTT
  CACCTGCGCGCTGGGCGCGTCCCCGGACGGGGCGGCAGGTGCGGGGCCCGCGGTGCGCGTGAGCTGCAAG
  CTCCCGTCCGTGCAACTGCAGCGCATCATCGCGGGCGTGGACATCGTGCTGCTGTGCGTCATGAACCTCA
  TCATCCTCGTCAACCTCATCCACCTCTTCATCTTCCGCAAGAGCAACTTCATCTTCGACAAGCTGCACAA
  GGTGGGCATCAAGACGCGCCGGCAGTGGCGCCGCTCGCAGTTCTGCGACATCAACATCCTGGCCATGTTC
  TGCAACGAGAACCGCGACCACATCAAGTCGCTCAACCGGCTGGACTTCATCACCAACGAGAGCGACCTCA
  TGTACGACAACGTGGTCCGGCAGCTGCTGGCGGCGCTGGCGCAGTCCAACCACGACGCCACCCCCACGGT
  GCGCGACTCGGGGGTGCAGACCGTGGACCCCAGCGCCAACCCCGCCGAGCCCGACGGCGCCGCCGAGCCG
  CCCGTGGTCAAGCGGCCGCGCAAGAAGATGAAGTGGATCCCCACCAGCAACCCGCTTCCGCAGCCCTTCA
  AGGAGCCGCTGGCCATCATGCGCGTGGAGAACAGCAAGGCGGAGAAGCCGAAGCCCGCGCGCAGGAAGAC
  GGCCACGGACACGCTGATCGCGCCGCTGCTGGACCGCTCCGCCCACCACTACAAGGGCGGAGGGGGCGAC
  CCGGGCCCCGGCCCCGCCCCTGCCCCCGCCCCGCCGCCCGCCCCTGACAAGAAGCACGCGCGCCACTTCT
  CCCTGGACGTGCACCCCTACATCCTCGGCACCAAGAAGGCCAAGGCCGAGGCGGTGCCCGCCGCCCTGCC
  CGCCTCCCGGAGCCAGGAGGGGGGCTTCCTGTCCCAGGCGGAGGACTGTGGGCTAGGCCTGGCCCCGGCG
  CCCATCAAAGATGCTCCGCTCCCCGAGAAGGAAATCCCGTACCCCACAGAGCCAGCCCGGGCAGGGCTTC
  CCTCGGGGGGCCCGTTCCACGTCCGCTCACCTCCCGCCGCCCCTGCTGTGGCCCCTCTGACACCAGCCAG
  CCTGGGCAAGGCGGAGCCCCTCACCATCCTGAGCCGAAACGCCACACACCCGCTGCTGCACATCAACACG
  CTGTACGAGGCCCGGGAGGAGGAGGACGGGGGCCCCCGCCTGCCGCAGGACGTGGGGGACCTCATCGCCA
  TCCCTGCCCCACAGCAGATCCTCATCGCCACCTTCGACGAGCCGAGAACGGTCGTGAGTACTGTGGAGTT
  T
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC213641 representing NM_052839
  Red=Cloning site Green=Tags(s)
  
  MHHLLEQSADMATALLAGEKLRELILPGAQDDKAGALAALLLQLKLELPFDRVVTIGTVLVPILLVTLVF
  TKNFAEEPIYCYTPHNFTRDQALYARGYCWTELRDALPGVDASLWPSLFEHKFLPYALLAFAAIMYVPAL
  GWEFLASTRLTSELNFLLQEIDNCYHRAAEGRAPKIEKQIQSKGPGITEREKREIIENAEKEKSPEQNLF
  EKYLERRGRSNFLAKLYLARHVLILLLSAVPISYLCTYYATQKQNEFTCALGASPDGAAGAGPAVRVSCK
  LPSVQLQRIIAGVDIVLLCVMNLIILVNLIHLFIFRKSNFIFDKLHKVGIKTRRQWRRSQFCDINILAMF
  CNENRDHIKSLNRLDFITNESDLMYDNVVRQLLAALAQSNHDATPTVRDSGVQTVDPSANPAEPDGAAEP
  PVVKRPRKKMKWIPTSNPLPQPFKEPLAIMRVENSKAEKPKPARRKTATDTLIAPLLDRSAHHYKGGGGD
  PGPGPAPAPAPPPAPDKKHARHFSLDVHPYILGTKKAKAEAVPAALPASRSQEGGFLSQAEDCGLGLAPA
  PIKDAPLPEKEIPYPTEPARAGLPSGGPFHVRSPPAAPAVAPLTPASLGKAEPLTILSRNATHPLLHINT
  LYEAREEEDGGPRLPQDVGDLIAIPAPQQILIATFDEPRTVVSTVEF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_052839
• ORF Size: 2031 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_052839.4
• RefSeq Size: 3069 bp
• RefSeq ORF: 2034 bp
• Locus ID: 56666
• UniProt ID: Q96RD6
• Cytogenetics: 22q13.33
• Protein Families: Transmembrane
• MW: 74.3 kDa
• Gene Summary: The protein encoded by this gene belongs to the innexin family. Innexin family members are the structural components of gap junctions. This protein and pannexin 1 are abundantly expressed in central nervous system (CNS) and are coexpressed in various neuronal populations. Studies in Xenopus oocytes suggest that this protein alone and in combination with pannexin 1 may form cell type-specific gap junctions with distinct properties. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]