TIAM2 (NM_001010927) Human Tagged ORF Clone
CAT#: RC213556
TIAM2 (Myc-DDK-tagged)-Human T-cell lymphoma invasion and metastasis 2 (TIAM2), transcript variant 2
ORF Plasmid: tGFP
Lentiviral Particles: DDK DDK w/ Puro mGFP mGFP w/ Puro
"NM_001010927" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | TIAM2 |
Synonyms | STEF; TIAM-2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC213556 representing NM_001010927
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAGGACCGCGGGAGAATCAGGATCCTCCTCCGAGGCCTCTGGCCCGCCACCTGTCTGATGCAGACC GCCTCCGCAAAGTCATCCAGGAGCTTGTGGACACAGAGAAGTCCTACGTGAAGGATTTGAGCTGCCTCTT TGAATTATACTTGGAGCCACTTCAGAATGAGACCTTTCTTACCCAAGATGAGATGGAGTCACTTTTTGGA AGTTTGCCAGAGATGCTTGAGTTTCAGAAGGTGTTTCTGGAGACCCTGGAGGATGGGATTTCAGCATCAT CTGACTTTAACACCCTAGAAACCCCCTCACAGTTTAGAAAATTACTGTTTTCCCTTGGAGGCTCTTTCCT TTATTACGCGGACCACTTTAAACTGTACAGTGGATTCTGTGCTAACCATATCAAAGTACAGAAGGTTCTG GAGCGAGCTAAAACTGACAAAGCCTTCAAGGCTTTTCTGGACGCCCGGAACCCCACCAAGCAGCATTCCT CCACGCTGGAGTCCTACCTCATCAAGCCGGTTCAGAGAGTGCTCAAGTACCCGCTGCTGCTCAAGGAGCT GGTGTCCCTGACGGACCAGGAGAGCGAGGAGCACTACCACCTGACGGAAGCACTAAAGGCAATGGAGAAA GTAGCGAGCCACATCAATGAGATGCAGAAGATCTATGAGGATTATGGGACCGTGTTTGACCAGCTAGTAG CTGAGCAGAGCGGAACAGAGAAGGAGGTAACAGAACTTTCGATGGGAGAGCTTCTGATGCACTCTACGGT TTCCTGGTTGAATCCATTTCTGTCTCTAGGAAAAGCTAGAAAGGACCTTGAGCTCACAGTATTTGTTTTT AAGAGAGCCGTCATACTGGTTTATAAAGAAAACTGCAAACTGAAAAAGAAATTGCCCTCGAATTCCCGGC CTGCACACAACTCTACTGACTTGGACCCATTTAAATTCCGCTGGTTGATCCCCATCTCCGCGCTTCAAGT CAGACTGGGGAATCCAGCAGGGACAGAAAATAATTCCATATGGGAACTGATCCATACGAAGTCAGAAATA GAAGGACGGCCAGAAACCATCTTTCAGTTGTGTTGCAGTGACAGTGAAAGCAAAACCAACATTGTTAAGG TGATTCGTTCTATTCTGAGGGAGAACTTCAGGCGTCACATAAAGTGTGAATTACCACTGGAGAAAACGTG TAAGGATCGCCTGGTACCTCTTAAGAACCGAGTTCCTGTTTCGGCCAAATTAGCTTCATCCAGGTCTTTA AAAGTCCTGAAGAATTCCTCCAGCAACGAGTGGACCGGTGAGACTGGCAAGGGAACCTTGCTGGACTCTG ACGAGGGCAGCTTGAGCAGCGGCACCCAGAGCAGCGGCTGCCCCACGGCTGAGGGCAGGCAGGACTCCAA GAGCACTTCTCCCGGGAAATACCCACACCCCGGCTTGGCAGATTTTGCCGACAATCTCATCAAAGAGAGT GACATCCTGAGCGATGAAGATGATGACCACCGTCAGACTGTGAAGCAGGGCAGCCCTACTAAAGACATCG AAATTCAGTTCCAGAGACTGAGGATTTCCGAGGACCCAGACGTTCACCCCGAGGCTGAGCAGCAGCCTGG CCCGGAGTCGGGTGAGGGTCAGAAAGGAGGAGAGCAGCCCAAACTGGTCCGGGGGCACTTCTGCCCCATT AAACGAAAAGCCAACAGCACCAAGAGGGACAGAGGAACTTTGCTCAAGGCGCAGATCCGTCACCAGTCCC TTGACAGTCAGTCTGAAAATGCCACCATCGACCTAAATTCTGTTCTAGAGCGAGAATTCAGTGTCCAGAG TTTAACATCTGTTGTCAGTGAGGAGTGTTTTTATGAAACAGAGAGCCACGGAAAATCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC213556 representing NM_001010927
Red=Cloning site Green=Tags(s) MEGPRENQDPPPRPLARHLSDADRLRKVIQELVDTEKSYVKDLSCLFELYLEPLQNETFLTQDEMESLFG SLPEMLEFQKVFLETLEDGISASSDFNTLETPSQFRKLLFSLGGSFLYYADHFKLYSGFCANHIKVQKVL ERAKTDKAFKAFLDARNPTKQHSSTLESYLIKPVQRVLKYPLLLKELVSLTDQESEEHYHLTEALKAMEK VASHINEMQKIYEDYGTVFDQLVAEQSGTEKEVTELSMGELLMHSTVSWLNPFLSLGKARKDLELTVFVF KRAVILVYKENCKLKKKLPSNSRPAHNSTDLDPFKFRWLIPISALQVRLGNPAGTENNSIWELIHTKSEI EGRPETIFQLCCSDSESKTNIVKVIRSILRENFRRHIKCELPLEKTCKDRLVPLKNRVPVSAKLASSRSL KVLKNSSSNEWTGETGKGTLLDSDEGSLSSGTQSSGCPTAEGRQDSKSTSPGKYPHPGLADFADNLIKES DILSDEDDDHRQTVKQGSPTKDIEIQFQRLRISEDPDVHPEAEQQPGPESGEGQKGGEQPKLVRGHFCPI KRKANSTKRDRGTLLKAQIRHQSLDSQSENATIDLNSVLEREFSVQSLTSVVSEECFYETESHGKS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001010927 |
ORF Size | 1878 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001010927.2, NP_001010927.1 |
RefSeq Size | 2668 bp |
RefSeq ORF | 1881 bp |
Locus ID | 26230 |
UniProt ID | Q8IVF5 |
Cytogenetics | 6q25.2-q25.3 |
Protein Families | Druggable Genome |
Protein Pathways | Chemokine signaling pathway, Regulation of actin cytoskeleton |
MW | 70.6 kDa |
Gene Summary | This gene encodes a guanine nucleotide exchange factor. A highly similar mouse protein specifically activates ras-related C3 botulinum substrate 1, converting this Rho-like guanosine triphosphatase (GTPase) from a guanosine diphosphate-bound inactive state to a guanosine triphosphate-bound active state. The encoded protein may play a role in neural cell development. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC213556L1 | Lenti ORF clone of Human T-cell lymphoma invasion and metastasis 2 (TIAM2), transcript variant 2, Myc-DDK-tagged |
USD 1,174.00 |
|
RC213556L2 | Lenti ORF clone of Human T-cell lymphoma invasion and metastasis 2 (TIAM2), transcript variant 2, mGFP tagged |
USD 1,174.00 |
|
RC213556L3 | Lenti ORF clone of Human T-cell lymphoma invasion and metastasis 2 (TIAM2), transcript variant 2, Myc-DDK-tagged |
USD 1,174.00 |
|
RC213556L4 | Lenti ORF clone of Human T-cell lymphoma invasion and metastasis 2 (TIAM2), transcript variant 2, mGFP tagged |
USD 1,174.00 |
|
RG213556 | TIAM2 (tGFP-tagged) - Human T-cell lymphoma invasion and metastasis 2 (TIAM2), transcript variant 2 |
USD 1,074.00 |
|
SC301537 | TIAM2 (untagged)-Human T-cell lymphoma invasion and metastasis 2 (TIAM2), transcript variant 2 |
USD 642.00 |
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