RECK (NM_021111) Human Tagged ORF Clone

CAT#: RC212174

RECK (Myc-DDK-tagged)-Human reversion-inducing-cysteine-rich protein with kazal motifs (RECK)

Product Images

Circular map for RC212174

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RECK
• Synonyms: ST15
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC212174 representing NM_021111
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGACCGTCCGGGCCTCTCTGCGAGGTGCGCTGCTCCTTCTGCTGGCCGTGGCGGGGGTCGCGGAGG
  TGGCAGGGGGCCTGGCTCCGGGCAGTGCGGGTGCATTGTGTTGTAATCATTCAAAGGATAACCAAATGTG
  CCGTGATGTATGTGAACAGATTTTCTCCTCAAAAAGTGAATCCCGACTAAAACATCTGTTGCAGCGAGCC
  CCAGATTATTGCCCAGAGACAATGGTTGAAATTTGGAATTGTATGAATTCATCTTTGCCAGGTGTGTTTA
  AGAAGTCTGATGGCTGGGTTGGCTTAGGCTGCTGTGAACTGGCTATTGCCTTGGAGTGTCGACAGGCATG
  CAAGCAGGCATCTTCAAAGAATGATATTTCCAAAGTTTGCAGAAAAGAATATGAGAATGCTCTTTTCAGT
  TGCATTAGCAGAAATGAAATGGGCTCGGTTTGTTGCAGTTATGCAGGTCATCACACAAACTGCCGAGAAT
  ACTGTCAAGCCATTTTTCGAACAGACTCTTCTCCTGGTCCATCTCAGATAAAAGCAGTGGAAAATTATTG
  CGCCTCTATTAGTCCACAATTAATACATTGTGTGAACAATTATACTCAATCTTATCCAATGAGGAACCCA
  ACGGATAGTTTATATTGCTGTGACAGAGCTGAAGACCATGCTTGCCAAAATGCCTGCAAGAGAATCCTGA
  TGTCCAAGAAAACGGAAATGGAGATTGTTGATGGTCTCATCGAGGGTTGTAAGACCCAGCCCTTGCCTCA
  AGATCCTCTTTGGCAATGTTTTCTTGAAAGCTCACAATCTGTTCACCCTGGAGTCACTGTACACCCTCCT
  CCCTCTACAGGCCTCGATGGGGCTAAATTGCATTGTTGTTCTAAAGCAAACACTTCAACATGTAGGGAAC
  TCTGCACTAAACTTTACAGCATGAGCTGGGGCAATACACAGAGTTGGCAAGAGTTTGATCGCTTTTGTGA
  ATATAATCCAGTGGAAGTGTCCATGTTGACCTGTTTAGCGGATGTCCGGGAACCTTGCCAGTTGGGCTGT
  AGAAACCTTACTTACTGTACTAATTTTAACAACAGGCCAACAGAACTTTTCAGGAGTTGTAATGCACAGT
  CAGATCAAGGAGCCATGAATGACATGAAGTTGTGGGAGAAAGGAAGCATAAAGATGCCATTTATCAATAT
  ACCTGTTCTTGATATTAAAAAGTGCCAGCCAGAGATGTGGAAAGCAATAGCTTGTTCACTGCAGATTAAA
  CCTTGTCATAGTAAATCTCGGGGAAGTATTATTTGCAAATCAGATTGTGTGGAGATTCTTAAAAAATGTG
  GAGACCAGAACAAATTCCCTGAAGACCACACAGCTGAAAGTATTTGTGAGCTTCTGTCACCTACAGATGA
  TCTGAAGAATTGTATACCTTTGGATACATACCTCAGGCCAAGTACTTTAGGTAACATTGTAGAAGAAGTG
  ACTCATCCCTGTAACCCAAATCCTTGCCCTGCCAATGAGCTCTGTGAAGTAAACCGAAAAGGATGTCCAT
  CTGGAGATCCCTGTCTTCCATACTTTTGTGTTCAAGGTTGCAAACTGGGAGAAGCTTCTGATTTCATTGT
  CCGTCAAGGGACACTAATCCAGGTGCCATCATCTGCAGGGGAAGTTGGTTGTTATAAAATCTGTTCATGT
  GGACAAAGTGGACTCTTAGAAAACTGTATGGAAATGCACTGTATAGACCTCCAGAAGTCTTGTATTGTTG
  GAGGAAAAAGAAAAAGTCATGGAACATCCTTTAGTATTGACTGCAATGTCTGTTCTTGTTTTGCTGGCAA
  TTTGGTGTGCTCTACCCGCCTTTGCCTCAGTGAGCACAGTTCAGAAGATGACCGTCGTACCTTCACAGGT
  CTGCCCTGTAACTGTGCAGATCAGTTTGTCCCTGTATGTGGGCAGAATGGGCGCACTTACCCCAGTGCCT
  GCATTGCTCGCTGTGTGGGCCTCCAAGACCATCAGTTTGAGTTTGGATCATGCATGTCAAAGGATCCATG
  TAATCCTAATCCCTGCCAAAAAAACCAAAGGTGCATACCCAAACCACAGGTCTGCCTGACGACTTTTGAT
  AAATTTGGATGTAGCCAGTATGAGTGTGTACCAAGACAGCTCGCGTGTGACCAGGTCCAAGATCCTGTTT
  GTGACACAGACCACATGGAGCACAACAATCTCTGCACTTTATACCAAAGAGGAAAAAGCCTCTCTTACAA
  AGGTCCCTGCCAGCCCTTTTGCAGAGCAACCGAGCCCGTATGTGGGCACAATGGTGAGACCTACAGCAGT
  GTGTGTGCTGCCTACTCGGATCGCGTGGCAGTCGATTACTATGGGGACTGCCAGGCCGTCGGAGTCCTCT
  CAGAGCACAGCTCCGTCGCCGAGTGTGCTTCTGTCAAGTGTCCTTCGCTCTTGGCAGCTGGATGCAAACC
  CATCATCCCACCGGGTGCTTGTTGCCCATTATGTGCTGGGATGTTAAGAGTTTTATTTGACAAAGAAAAA
  CTGGATACTATTGCTAAGGTAACAAATAAAAAGCCAATAACAGTTCTGGAAATACTTCAGAAAATCCGCA
  TGCACGTGTCTGTCCCACAGTGTGATGTGTTTGGATACTTCAGCATTGAATCAGAAATTGTGATCCTGAT
  CATTCCCGTCGATCACTATCCAAAAGCTCTGCAGATTGAAGCCTGCAATAAAGAAGCAGAGAAGATTGAG
  TCCCTTATCAACTCTGACAGCCCGACTTTGGCGTCCCATGTCCCTCTCTCTGCCCTCATCATTTCCCAGG
  TACAGGTCTCCAGCAGTGTGCCATCGGCCGGTGTCAGGGCCAGGCCTTCTTGCCACTCCCTCCTCCTTCC
  CCTCAGCTTGGGCCTTGCCTTGCACTTGCTCTGGACATATAAC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC212174 representing NM_021111
  Red=Cloning site Green=Tags(s)
  
  MATVRASLRGALLLLLAVAGVAEVAGGLAPGSAGALCCNHSKDNQMCRDVCEQIFSSKSESRLKHLLQRA
  PDYCPETMVEIWNCMNSSLPGVFKKSDGWVGLGCCELAIALECRQACKQASSKNDISKVCRKEYENALFS
  CISRNEMGSVCCSYAGHHTNCREYCQAIFRTDSSPGPSQIKAVENYCASISPQLIHCVNNYTQSYPMRNP
  TDSLYCCDRAEDHACQNACKRILMSKKTEMEIVDGLIEGCKTQPLPQDPLWQCFLESSQSVHPGVTVHPP
  PSTGLDGAKLHCCSKANTSTCRELCTKLYSMSWGNTQSWQEFDRFCEYNPVEVSMLTCLADVREPCQLGC
  RNLTYCTNFNNRPTELFRSCNAQSDQGAMNDMKLWEKGSIKMPFINIPVLDIKKCQPEMWKAIACSLQIK
  PCHSKSRGSIICKSDCVEILKKCGDQNKFPEDHTAESICELLSPTDDLKNCIPLDTYLRPSTLGNIVEEV
  THPCNPNPCPANELCEVNRKGCPSGDPCLPYFCVQGCKLGEASDFIVRQGTLIQVPSSAGEVGCYKICSC
  GQSGLLENCMEMHCIDLQKSCIVGGKRKSHGTSFSIDCNVCSCFAGNLVCSTRLCLSEHSSEDDRRTFTG
  LPCNCADQFVPVCGQNGRTYPSACIARCVGLQDHQFEFGSCMSKDPCNPNPCQKNQRCIPKPQVCLTTFD
  KFGCSQYECVPRQLACDQVQDPVCDTDHMEHNNLCTLYQRGKSLSYKGPCQPFCRATEPVCGHNGETYSS
  VCAAYSDRVAVDYYGDCQAVGVLSEHSSVAECASVKCPSLLAAGCKPIIPPGACCPLCAGMLRVLFDKEK
  LDTIAKVTNKKPITVLEILQKIRMHVSVPQCDVFGYFSIESEIVILIIPVDHYPKALQIEACNKEAEKIE
  SLINSDSPTLASHVPLSALIISQVQVSSSVPSAGVRARPSCHSLLLPLSLGLALHLLWTYN
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021111
• ORF Size: 2913 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021111.3
• RefSeq Size: 4414 bp
• RefSeq ORF: 2916 bp
• Locus ID: 8434
• UniProt ID: O95980
• Cytogenetics: 9p13.3
• Domains: kazal
• Protein Families: Transmembrane
• MW: 103.9 kDa
• Gene Summary: The protein encoded by this gene is a cysteine-rich, extracellular protein with protease inhibitor-like domains whose expression is suppressed strongly in many tumors and cells transformed by various kinds of oncogenes. In normal cells, this membrane-anchored glycoprotein may serve as a negative regulator for matrix metalloproteinase-9, a key enzyme involved in tumor invasion and metastasis. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2015]