CSTL1 (NM_138283) Human Tagged ORF Clone
CAT#: RC211515
CSTL1 (Myc-DDK-tagged)-Human cystatin-like 1 (CSTL1)
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_138283" in other vectors (4)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | CSTL1 |
Synonyms | CTES1; dJ322G13.4; RCET11 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC211515 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGATCGGATGCTGGAGAAACCCCCTGCTGCTGCTGATTGCCCTGGTCCTGTCAGCCAAGCTGGGTC ACTTCCAAAGGTGGGAGGGCTTCCAGCAGAAGCTCATGAGCAAGAAGAACATGAATTCAACACTCAACTT CTTCATTCAATCCTACAACAATGCCAGCAACGACACCTACTTATATCGAGTCCAGAGGCTAATTCGAAGT CAGATGCAGCTGACGACGGGAGTGGAGTATATAGTCACTGTGAAGATTGGCCGGACCAAATGCAAGAGGA ATGACACGAGCAATTCTTCCTGCCCCCTGCAAAGCAAGAAGCTGAGAAAGAGTTTAATTTGCGAGTCTTT GATATACACCATGCCCTGGATAAACTATTTCCAGCTCTGGAACAATTCCTGTCTGGAGGCCGAGCATGTG GGCAGAAACCTCAGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC211515 protein sequence
Red=Cloning site Green=Tags(s) MGIGCWRNPLLLLIALVLSAKLGHFQRWEGFQQKLMSKKNMNSTLNFFIQSYNNASNDTYLYRVQRLIRS QMQLTTGVEYIVTVKIGRTKCKRNDTSNSSCPLQSKKLRKSLICESLIYTMPWINYFQLWNNSCLEAEHV GRNLR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_138283 |
ORF Size | 435 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_138283.1, NP_612140.1 |
RefSeq Size | 736 bp |
RefSeq ORF | 438 bp |
Locus ID | 128817 |
UniProt ID | Q9H114 |
Cytogenetics | 20p11.21 |
Protein Families | Secreted Protein |
MW | 17 kDa |
Gene Summary | The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions. The cystatin locus on chromosome 20 contains the majority of the type 2 cystatin genes and pseudogenes. This gene is located at the telomeric end of the cystatin locus and encodes a type 2 cystatin-like protein. The specific function of this protein has not been determined. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC211515L3 | Lenti ORF clone of Human cystatin-like 1 (CSTL1), Myc-DDK-tagged |
USD 450.00 |
|
RC211515L4 | Lenti ORF clone of Human cystatin-like 1 (CSTL1), mGFP tagged |
USD 450.00 |
|
RG211515 | CSTL1 (tGFP-tagged) - Human cystatin-like 1 (CSTL1) |
USD 350.00 |
|
SC305998 | CSTL1 (untagged)-Human cystatin-like 1 (CSTL1) |
USD 165.00 |
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