ENT2 (SLC29A2) (NM_001532) Human Tagged ORF Clone
CAT#: RC210776
SLC29A2 (Myc-DDK-tagged)-Human solute carrier family 29 (nucleoside transporters), member 2 (SLC29A2)
ORF Plasmid: tGFP
Lentiviral Particles: DDK DDK w/ Puro mGFP mGFP w/ Puro
AAV Particle: DDK
"NM_001532" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | ENT2 |
Synonyms | DER12; ENT2; HNP36 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC210776 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCGAGGAGACGCCCCGCGGGACAGCTACCACCTGGTCGGGATCAGCTTCTTCATCCTGGGGCTGG GCACCCTCCTTCCCTGGAACTTCTTCATCACCGCCATCCCGTACTTCCAGGCGCGACTGGCCGGGGCCGG CAACAGCACAGCCAGGATCCTGAGCACCAACCACACGGGTCCCGAGGATGCCTTCAACTTCAACAATTGG GTGACGCTGCTGTCCCAGCTGCCCCTGCTGCTCTTCACCCTCCTCAACTCCTTCCTGTACCAGTGCGTCC CGGAGACGGTGCGCATTCTGGGCAGCCTGCTGGCCATACTGCTGCTCTTTGCCCTGACAGCAGCGCTGGT CAAGGTGGACATGAGCCCCGGACCCTTCTTCTCCATCACCATGGCCTCCGTCTGCTTCATCAACTCCTTC AGTGCAGTCCTACAGGGCAGCCTCTTCGGGCAGCTGGGCACCATGCCCTCCACCTACAGCACCCTCTTCC TCAGCGGCCAGGGCCTGGCTGGGATCTTTGCTGCCCTTGCCATGCTCCTGTCCATGGCCAGTGGCGTGGA CGCCGAGACCTCTGCCCTGGGGTACTTTATCACGCCCTGTGTGGGCATCCTCATGTCCATCGTGTGTTAC CTGAGCCTGCCTCACCTGAAGTTTGCCCGCTACTACCTGGCCAATAAATCATCCCAGGCCCAAGCTCAGG AGCTGGAGACCAAAGCTGAGCTCCTCCAGTCTGATGAGAACGGGATTCCCAGTAGTCCCCAGAAAGTAGC TCTGACCCTGGATCTTGACCTGGAGAAGGAGCCGGAATCAGAGCCAGATGAGCCCCAGAAGCCAGGAAAA CCTTCAGTCTTCACTGTCTTCCAGAAGATCTGGCTGACAGCGCTGTGCCTTGTGTTGGTCTTCACAGTCA CCCTGTCCGTCTTCCCCGCCATCACAGCCATGGTGACCAGCTCCACCAGTCCTGGGAAGTGGAGTCAGTT CTTCAACCCCATCTGCTGCTTCCTCCTCTTCAACATCATGGACTGGCTGGGACGGAGCCTGACCTCTTAC TTCCTGTGGCCAGACGAGGACAGCCGGCTGCTGCCCCTGCTGGTCTGCCTGCGGTTCCTGTTCGTGCCCC TCTTCATGCTGTGCCACGTGCCCCAGAGGTCCCGGCTGCCCATCCTCTTCCCACAGGATGCCTACTTCAT CACCTTCATGCTGCTCTTTGCCGTTTCTAATGGCTACCTGGTGTCCCTCACCATGTGCCTGGCGCCCAGG CAGGTGCTGCCACACGAGAGGGAGGTGGCCGGCGCCCTCATGACCTTCTTCCTGGCCCTGGGACTTTCCT GTGGAGCCTCCCTCTCCTTCCTCTTCAAGGCGCTGCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC210776 protein sequence
Red=Cloning site Green=Tags(s) MARGDAPRDSYHLVGISFFILGLGTLLPWNFFITAIPYFQARLAGAGNSTARILSTNHTGPEDAFNFNNW VTLLSQLPLLLFTLLNSFLYQCVPETVRILGSLLAILLLFALTAALVKVDMSPGPFFSITMASVCFINSF SAVLQGSLFGQLGTMPSTYSTLFLSGQGLAGIFAALAMLLSMASGVDAETSALGYFITPCVGILMSIVCY LSLPHLKFARYYLANKSSQAQAQELETKAELLQSDENGIPSSPQKVALTLDLDLEKEPESEPDEPQKPGK PSVFTVFQKIWLTALCLVLVFTVTLSVFPAITAMVTSSTSPGKWSQFFNPICCFLLFNIMDWLGRSLTSY FLWPDEDSRLLPLLVCLRFLFVPLFMLCHVPQRSRLPILFPQDAYFITFMLLFAVSNGYLVSLTMCLAPR QVLPHEREVAGALMTFFLALGLSCGASLSFLFKALL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001532 |
ORF Size | 1368 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001532.3 |
RefSeq Size | 2529 bp |
RefSeq ORF | 1371 bp |
Locus ID | 3177 |
UniProt ID | Q14542 |
Cytogenetics | 11q13.2 |
Protein Families | Transmembrane |
MW | 50.1 kDa |
Gene Summary | The uptake of nucleosides by transporters, such as SLC29A2, is essential for nucleotide synthesis by salvage pathways in cells that lack de novo biosynthetic pathways. Nucleoside transport also plays a key role in the regulation of many physiologic processes through its effect on adenosine concentration at the cell surface (Griffiths et al., 1997 [PubMed 9396714]).[supplied by OMIM, Nov 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC210776L1 | Lenti ORF clone of Human solute carrier family 29 (nucleoside transporters), member 2 (SLC29A2), Myc-DDK-tagged |
USD 757.00 |
|
RC210776L2 | Lenti ORF clone of Human solute carrier family 29 (nucleoside transporters), member 2 (SLC29A2), mGFP tagged |
USD 757.00 |
|
RC210776L3 | Lenti ORF clone of Human solute carrier family 29 (nucleoside transporters), member 2 (SLC29A2), Myc-DDK-tagged |
USD 757.00 |
|
RC210776L4 | Lenti ORF clone of Human solute carrier family 29 (nucleoside transporters), member 2 (SLC29A2), mGFP tagged |
USD 757.00 |
|
RG210776 | SLC29A2 (tGFP-tagged) - Human solute carrier family 29 (nucleoside transporters), member 2 (SLC29A2) |
USD 657.00 |
|
SC122599 | SLC29A2 (untagged)-Human solute carrier family 29 (nucleoside transporters), member 2 (SLC29A2) |
USD 457.00 |
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