SERPINA6 (NM_001756) Human Tagged ORF Clone

CAT#: RC208873

SERPINA6 (Myc-DDK-tagged)-Human serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 6 (SERPINA6)

Product Images

Circular map for RC208873

• 

  Western blot validation of overexpression lysate (Cat# LY419763) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC208873 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SERPINA6
• Synonyms: CBG
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208873 representing NM_001756
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCACTCCTCCTGTACACCTGTCTTCTCTGGCTGCCCACCAGCGGCCTCTGGACCGTCCAGGCCATGG
  ATCCTAACGCTGCTTATGTGAACATGAGTAACCATCACCGGGGCCTGGCTTCAGCCAACGTTGACTTTGC
  CTTCAGCCTGTATAAGCACCTAGTGGCCTTGAGTCCCAAAAAGAACATTTTCATCTCCCCTGTGAGCATC
  TCCATGGCCTTAGCTATGCTGTCCCTGGGCACCTGTGGCCACACACGGGCCCAGCTTCTCCAGGGCCTGG
  GTTTCAACCTCACTGAGAGGTCTGAGACTGAGATCCACCAGGGTTTCCAGCACCTGCACCAACTCTTTGC
  AAAGTCAGACACCAGCTTAGAAATGACCATGGGCAATGCCTTGTTTCTTGATGGCAGCCTGGAGTTGCTG
  GAGTCATTCTCAGCAGACATCAAGCACTACTATGAGTCAGAGGTCTTGGCTATGAATTTCCAGGACTGGG
  CAACAGCCAGCAGACAGATCAACAGCTATGTCAAGAATAAGACACAGGGGAAAATTGTCGACTTGTTTTC
  AGGGCTGGATAGCCCAGCCATCCTCGTCCTGGTCAACTATATCTTCTTCAAAGGCACATGGACACAGCCC
  TTTGACCTGGCAAGCACCAGGGAGGAGAACTTCTATGTGGACGAGACAACTGTGGTGAAGGTGCCCATGA
  TGTTGCAGTCGAGCACCATCAGTTACCTTCATGACGCGGAGCTCCCCTGCCAGCTGGTGCAGATGAACTA
  CGTGGGCAATGGGACTGTCTTCTTCATCCTTCCGGACAAGGGGAAGATGAACACAGTCATCGCTGCACTG
  AGCCGGGACACGATTAACAGGTGGTCCGCAGGCCTGACCAGCAGCCAGGTGGACCTGTACATTCCAAAGG
  TCACCATCTCTGGAGTCTATGACCTCGGAGATGTGCTGGAGGAAATGGGCATTGCAGACTTGTTCACCAA
  CCAGGCAAATTTCTCACGCATCACCCAGGACGCCCAGCTGAAGTCATCAAAGGTGGTCCATAAAGCTGTG
  CTGCAACTCAATGAGGAGGGTGTGGACACAGCTGGCTCCACTGGGGTCACCCTAAACCTGACGTCCAAGC
  CTATCATCTTGCGTTTCAACCAGCCCTTCATCATCATGATCTTCGACCACTTCACCTGGAGCAGCCTTTT
  CCTGGCGAGGGTTATGAACCCAGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208873 representing NM_001756
  Red=Cloning site Green=Tags(s)
  
  MPLLLYTCLLWLPTSGLWTVQAMDPNAAYVNMSNHHRGLASANVDFAFSLYKHLVALSPKKNIFISPVSI
  SMALAMLSLGTCGHTRAQLLQGLGFNLTERSETEIHQGFQHLHQLFAKSDTSLEMTMGNALFLDGSLELL
  ESFSADIKHYYESEVLAMNFQDWATASRQINSYVKNKTQGKIVDLFSGLDSPAILVLVNYIFFKGTWTQP
  FDLASTREENFYVDETTVVKVPMMLQSSTISYLHDAELPCQLVQMNYVGNGTVFFILPDKGKMNTVIAAL
  SRDTINRWSAGLTSSQVDLYIPKVTISGVYDLGDVLEEMGIADLFTNQANFSRITQDAQLKSSKVVHKAV
  LQLNEEGVDTAGSTGVTLNLTSKPIILRFNQPFIIMIFDHFTWSSLFLARVMNPV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001756
• ORF Size: 1215 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001756.3, NP_001747.2
• RefSeq Size: 1510 bp
• RefSeq ORF: 1218 bp
• Locus ID: 866
• UniProt ID: P08185
• Cytogenetics: 14q32.13
• Domains: SERPIN
• Protein Families: Druggable Genome, Secreted Protein
• MW: 45.12 kDa
• Gene Summary: This gene encodes an alpha-globulin protein with corticosteroid-binding properties. This is the major transport protein for glucorticoids and progestins in the blood of most vertebrates. The gene localizes to a chromosomal region containing several closely related serine protease inhibitors which may have evolved by duplication events. [provided by RefSeq, Jul 2008]