VPS4A (NM_013245) Human Tagged ORF Clone

CAT#: RC208099

VPS4A (Myc-DDK-tagged)-Human vacuolar protein sorting 4 homolog A (S. cerevisiae) (VPS4A)

Product Images

Circular map for RC208099

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  Western blot validation of overexpression lysate (Cat# LY415707) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC208099 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified VPS4A protein (Cat# TP308099). The protein was produced from HEK293T cells transfected with VPS4A cDNA clone (Cat# RC208099) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: VPS4A
• Synonyms: CIMDAG; SKD1; SKD1A; SKD2; VPS4; VPS4-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208099 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACAACGTCAACCCTCCAGAAAGCCATTGATCTGGTGACGAAAGCCACAGAGGAGGACAAAGCCAAGA
  ACTACGAGGAGGCGCTGCGGCTGTACCAGCATGCGGTGGAGTACTTCCTCCACGCTATCAAGTATGAGGC
  CCACAGCGACAAGGCCAAGGAGAGCATTCGAGCCAAGTGCGTGCAGTACCTAGACCGGGCCGAGAAGCTG
  AAGGATTATTTACGAAGCAAAGAGAAACACGGCAAGAAGCCAGTCAAAGAGAACCAGAGTGAGGGCAAGG
  GCAGTGACAGTGACAGTGAAGGGGATAATCCGGAGAAAAAGAAACTGCAAGAACAGCTGATGGGTGCCGT
  CGTGATGGAGAAGCCCAACATACGGTGGAACGACGTGGCCGGGCTGGAGGGGGCCAAGGAGGCCCTCAAA
  GAAGCTGTCATTTTGCCAATCAAATTCCCACACTTGTTCACAGGCAAGCGCACCCCCTGGCGGGGGATTC
  TGCTGTTCGGACCCCCTGGCACAGGGAAATCCTACCTGGCCAAAGCCGTGGCAACAGAGGCCAACAACTC
  CACCTTCTTCTCTGTGTCCTCCTCAGATCTGATGTCCAAGTGGCTGGGGGAGAGTGAAAAGCTGGTCAAG
  AACCTGTTTGAGCTGGCCAGGCAGCACAAGCCCTCCATCATCTTCATCGATGAGGTGGATTCCCTCTGCG
  GGTCCCGAAATGAAAATGAGAGTGAGGCCGCCCGGAGGATCAAAACGGAGTTCTTGGTCCAGATGCAGGG
  GGTGGGGAATAACAATGATGGGACTCTGGTTCTTGGAGCCACAAACATCCCATGGGTGTTGGATTCGGCC
  ATCAGGAGGAGGTTTGAAAAACGAATTTATATCCCCTTGCCGGAGGAAGCTGCCCGCGCCCAGATGTTCC
  GGTTGCATCTCGGGAGCACTCCCCACAACCTCACGGATGCAAACATCCACGAGCTGGCCCGGAAGACGGA
  AGGCTACTCGGGCGCGGACATCAGCATCATCGTGCGGGACTCTCTCATGCAGCCCGTGAGGAAGGTGCAG
  TCGGCCACACACTTCAAAAAGGTCTGTGGCCCCTCTCGCACCAACCCCAGCATGATGATTGATGACCTCC
  TGACTCCATGCTCACCAGGGGACCCAGGAGCCATGGAGATGACTTGGATGGATGTCCCTGGGGACAAACT
  CTTAGAGCCTGTGGTTTGCATGTCGGACATGCTGCGGTCTCTGGCCACCACCCGGCCCACGGTGAATGCA
  GACGACCTCCTGAAAGTGAAGAAATTCTCAGAGGACTTTGGGCAAGAGAGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208099 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MTTSTLQKAIDLVTKATEEDKAKNYEEALRLYQHAVEYFLHAIKYEAHSDKAKESIRAKCVQYLDRAEKL
  KDYLRSKEKHGKKPVKENQSEGKGSDSDSEGDNPEKKKLQEQLMGAVVMEKPNIRWNDVAGLEGAKEALK
  EAVILPIKFPHLFTGKRTPWRGILLFGPPGTGKSYLAKAVATEANNSTFFSVSSSDLMSKWLGESEKLVK
  NLFELARQHKPSIIFIDEVDSLCGSRNENESEAARRIKTEFLVQMQGVGNNNDGTLVLGATNIPWVLDSA
  IRRRFEKRIYIPLPEEAARAQMFRLHLGSTPHNLTDANIHELARKTEGYSGADISIIVRDSLMQPVRKVQ
  SATHFKKVCGPSRTNPSMMIDDLLTPCSPGDPGAMEMTWMDVPGDKLLEPVVCMSDMLRSLATTRPTVNA
  DDLLKVKKFSEDFGQES
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013245
• ORF Size: 1311 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013245.3
• RefSeq Size: 2211 bp
• RefSeq ORF: 1314 bp
• Locus ID: 27183
• UniProt ID: Q9UN37
• Cytogenetics: 16q22.1
• Domains: AAA, AAA, MIT
• Protein Pathways: Endocytosis
• MW: 48.9 kDa
• Gene Summary: The protein encoded by this gene is a member of the AAA protein family (ATPases associated with diverse cellular activities), and is the homolog of the yeast Vps4 protein. In humans, two paralogs of the yeast protein have been identified. The former share a high degree of aa sequence similarity with each other, and also with yeast Vps4 and mouse Skd1 proteins. The mouse Skd1 (suppressor of K+ transport defect 1) has been shown to be really an yeast Vps4 ortholog. Functional studies indicate that both human paralogs associate with the endosomal compartments, and are involved in intracellular protein trafficking, similar to Vps4 protein in yeast. The gene encoding this paralog has been mapped to chromosome 16; the gene for the other resides on chromosome 18. [provided by RefSeq, Jul 2008]