RNF185 (NM_152267) Human Tagged ORF Clone
CAT#: RC207827
RNF185 (Myc-DDK-tagged)-Human ring finger protein 185 (RNF185), transcript variant 1
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_152267" in other vectors (4)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | RNF185 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC207827 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAAGCAAGGGGCCCTCGGCCTCTGCATCTCCTGAGAACTCCAGTGCAGGGGGGCCCAGTGGGAGCA GCAATGGCGCTGGCGAGAGCGGAGGGCAGGACAGCACTTTCGAGTGCAACATCTGCTTGGACACAGCCAA GGATGCCGTCATCAGCCTGTGTGGCCACCTCTTCTGTTGGCCGTGTTTACATCAGTGGTTGGAGACCAGA CCTAACAGACAGGTGTGTCCTGTTTGCAAAGCTGGCATCAGCCGAGACAAGGTCATCCCCCTCTATGGAA GGGGCAGCACTGGGCAACAGGACCCCAGAGAGAAGACCCCTCCTCGTCCTCAAGGACAGAGGCCAGAGCC GGAGAATAGAGGGGGATTTCAAGGATTTGGATTTGGAGATGGTGGCTTCCAGATGTCTTTTGGAATTGGG GCATTTCCCTTTGGGATATTTGCCACAGCATTTAATATAAATGATGGGCGGCCTCCTCCAGCTGTCCCTG GGACACCCCAGTATGTGGACGAGCAGTTCCTGTCACGCCTCTTCCTATTTGTGGCCCTGGTGATCATGTT CTGGCTCCTGATTGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC207827 protein sequence
Red=Cloning site Green=Tags(s) MASKGPSASASPENSSAGGPSGSSNGAGESGGQDSTFECNICLDTAKDAVISLCGHLFCWPCLHQWLETR PNRQVCPVCKAGISRDKVIPLYGRGSTGQQDPREKTPPRPQGQRPEPENRGGFQGFGFGDGGFQMSFGIG AFPFGIFATAFNINDGRPPPAVPGTPQYVDEQFLSRLFLFVALVIMFWLLIA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_152267 |
ORF Size | 576 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_152267.4 |
RefSeq Size | 3218 bp |
RefSeq ORF | 579 bp |
Locus ID | 91445 |
UniProt ID | Q96GF1 |
Cytogenetics | 22q12.2 |
Domains | RING |
Protein Families | Druggable Genome, Transmembrane |
MW | 20.5 kDa |
Gene Summary | E3 ubiquitin-protein ligase that regulates selective mitochondrial autophagy by mediating 'Lys-63'-linked polyubiquitination of BNIP1 (PubMed:21931693). Acts in the endoplasmic reticulum (ER)-associated degradation (ERAD) pathway, which targets misfolded proteins that accumulate in the endoplasmic reticulum (ER) for ubiquitination and subsequent proteasome-mediated degradation (PubMed:27485036). Protects cells from ER stress-induced apoptosis (PubMed:27485036). Responsible for the cotranslational ubiquitination and degradation of CFTR in the ERAD pathway (PubMed:24019521). Preferentially associates with the E2 enzymes UBE2J1 and UBE2J2 (PubMed:24019521).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC207827L3 | Lenti ORF clone of Human ring finger protein 185 (RNF185), transcript variant 1, Myc-DDK-tagged |
USD 600.00 |
|
RC207827L4 | Lenti ORF clone of Human ring finger protein 185 (RNF185), transcript variant 1, mGFP tagged |
USD 600.00 |
|
RG207827 | RNF185 (tGFP-tagged) - Human ring finger protein 185 (RNF185), transcript variant 1 |
USD 500.00 |
|
SC100933 | RNF185 (untagged)-Human ring finger protein 185 (RNF185), transcript variant 1 |
USD 300.00 |
{0} Product Review(s)
Be the first one to submit a review